The first molecular characterisation of Steinernema silvaticum recorded in Poland and its differentiation from Steinernema kraussei using ribosomal DNA (rDNA) sequences

• Autorzy: Dziegielewska M. , Berdzik M. , Myskow B.

Warianty tytułu

PL

Pierwsza charakterystyka molekularna Steinernema silvaticum wykrytego w Polsce i jego odróżnienie od Steinernema kraussei na podstawie sekwencji DNA rybosomalnego (rDNA)

• Języki publikacji: EN

Abstrakty

EN

During faunistic studies conducted in north-western Poland on the entomopathogenic nematodes (EPN) of the families Steinernematidae and Heterorhabditidae, species Steinernema silvaticum was identified for the first time in Poland. Nematode species identification, using morphometric methods, is laborious, even for an experienced researcher. S. silvaticum and S. kraussei are defined as “sister taxa” and it is difficult to distinguish these two species. The study is an attempt to differentiate S. silvaticum from S. kraussei using sequencing results of the rDNA – ITS1 and LSU regions. Sequenced regions of ITS1 and LSU had lengths of 490bp and 918bp, respectively. A very high similarity between the S. silvaticum isolate and the isolate of S. kraussei was detected in the case of both sequences (98–99%). There were only two nucleotide differences in ITS1 and two in LSU region, which discriminate the two analysed species.

PL

Podczas badań nad występowaniem nicieni owadobójczych (EPN) z rodzin Steinernematidae oraz Heterorhabditidae, prowadzonych w północno-zachodniej Polsce, po raz pierwszy w Polsce zidentyfikowano gatunek Steinernema silvaticum. Identyfikacja gatunkowa nicieni, zwłaszcza odróżnienie od siebie tych najpodobniejszych, określanych mianem gatunków „siostrzanych”, z wykorzystaniem metod morfometrycznych, jest pracochłonna i często problematyczna nawet dla doświadczonego badacza. Prezentowane badania są próbą odróżnienia S. silvaticum od S. kraussei przy zastosowaniu wyników sekwencjonowania rejonów rybosomalnego DNA (rDNA) – regionów ITS1 i LSU. Sekwencjonowane obszary miały długości odpowiednio 490 bp i 918 bp. Podobieństwo między izolatami S. silvaticum i S. kraussei w przypadku obydwu sekwencji było bardzo wysokie (98–99%). Dwa polimorficzne nukleotydy (SNP) w ITS1 i dwa w LSU pozwoliły na odróżnienie obu analizowanych gatunków.

• Wydawca: -
• Czasopismo: Folia Pomeranae Universitatis Technologiae Stetinensis. Agricultura, Alimentaria, Piscaria et Zootechnica
• Rocznik: 2015
• Tom: 33
• Opis fizyczny: p.41-47,ref.

Twórcy

autor

Dziegielewska M.

• Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology, Szczecin, Poland

autor

Berdzik M.

• Department of Genetics, Plant Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin, Poland

autor

Myskow B.

• Department of Genetics, Plant Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin, Juliusza Slowackiego 17, 71-434 Szczecin, Poland

Bibliografia

• Adams B.J., Burnell A.M., Powers T.O. 1998. A phylogenetic analysis of Heterorhabditis (Nemata: Rhabditidae) based on internal transcribed spacer 1 DNA sequence data. J Nematol 30(1), 22–39
• Ehlers R.U. 2001. Mass production of entomopathogenic nematodes for plant protection. Appl. Microbiol. Biot. 56, 623–633.
• Hall T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids. Symp. Ser. 41, 95–98.
• Hominick W.M. 2002. Biogeography. In: Gaugler R. Entomopathogenic Nematology, CABI Publishing, Wallingford, UK, ISBN 0851995675, 115–143.
• Hominick W.M., Reid A.P., Bohan D.A., Briscoe B.R. 1996. Entomopathogenic nematodes – biodiversity, geographical distribution and the convention on biological diversity. Biocontrol. Sci. Techn. 6, 317–331.
• Hominick W.M., Reid A.P., Briscoe B.R. 1995. Prevalence and habitat specificity of steinernematid and heterorhabditid nematodes isolated during soil surveys of the UK and the Netherlands. J. Helminthol. 69, 27–32.
• Karbowska-Dzięgielewska M. 2013. Ekologiczne uwarunkowania występowania nicieni owadobójczych (Steinernematidae, Heterorhabditidae) w wybranych ekosystemach północno-zachodniej Polski [Environmental determinants of the occurrence of entomopathogenic nematodes (Steinernematidae, Heterorhabditidae) in selected ecosystems of the north-western Poland]. Rozprawa habilitacyjna. Szczecin, Wydaw. Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego ISBN 978- -83-7663-142-4. [in Polish].
• Kaya H.K., Aguillera M.M., Alumai A., Choo H.Y., de la Torre M., Fodor A., Ganguly S., Hazâr S., Lakatos T., Pye A., Wilson M., Yamanaka S., Yang H., Ehlers R.U. 2006. Status of entomopathogenic nematodes and their symbiotic bacteria from selected countries or regions of the world. Biol. Control 38, 134–155.
• Kuwata R., Shigematsu M., Yoshiga T., Yoshida M., Kondo E. 2006. Phylogenetic analyses of Japanese steinernematid nematodes and their associating Xenorhabdus bacteria. Nihon Senchu Gakkai Shi 36, 75–85.
• Lee M.M., Stock S.P. 2010. A multilocus approach to assessing co-evolutionary relationships between Steinernema spp. (Nematoda: Steinernematidae) and their bacterial symbionts Xenorhabdus spp. (gamma-Proteobacteria: Enterobacteriaceae). Syst. Parasitol. 77(1), 1–12.
• Lewis E.E., Campbell J.F., Griffin C., Kaya H.K., Peters A. 2006. Behavioral ecology of entomopathogenic nematodes. Biol. Control 38, 66–79.
• Litvaitis M.K., Bates J.W., Hope W.D., Moens T. 2000. Inferring a classification of the Adenophorea (Nematoda) from nucleotide sequences of the D3 expansion segment (26/28S rDNA). Can. J. Zool. 78, 911–922. doi:10.1139/cjz-78-6-911.
• Mráček Z., Bečvář S., Kindlmann P. 1999. Survey of entomopathogenic nematodes from the families (Nematoda: Rhabditida) in the Czech Republic. Folia Parasit 46, 145–148.
• Mráček Z., Bečvář S. 2000. Insect aggregations and entomopathogenic nematode occurrence. Nematology 2(3), 297–301.
• Mráček Z., Sturhan D., Reid A.P. 2003. Steinernema weiseri n.sp. (Rhabditida, Steinernematidae), a new entomopathogenic nematode from Europe. Syst. Parasitol. 56, 37–47.
• Mráček Z., Bečvář S., Kindlmann P., Jersáková J. 2005. Habitat preference for entomopathogenic nematodes, their insect hosts and new faunistic records for the Czech Republic. Biol. Control 34, 27–37.
• Nadler S.A., Bolotin E., Stock P. 2006. Phylogenetic relationships of Steinernema Travassos, 1927 (Nematoda: Cephalobina: Steinernematidae) based on nuclear, mitochondrial and morphological data. Syst. Parasitol. 63, 161–181.
• Nasmith C.G., Speranzini D., Jeng R., Hubbes M. 1996. RFLP analysis of PCR amplified ITS and 26S ribosomal RNA genes of selected entomopathogenic nematodes (Steinernematidae, Heterorhabditidae) J. Nematol 28(1), 15–25.
• Nguyen K.B. 2007. Methodology, morphology and identification. In: Nguyen KB, Hunt DJ (Eds). Entomopathogenic nematode: Systematics, Phylogeny and Bacterial symbionts. Nematology Monographs and Perspectives. Vol. 5. Brill Leiden-Boston, 59–119.
• Půža V., Mráček Z. 2005. Seasonal dynamics of entomopathogenic nematodes of the genera Steinernema and Heterorhabditis as a response to abiotic factors and abundance of insect hosts. J. Invertebr. Pathol. 89, 116–122.
• Shapiro-Ilan D.I., Gouge D.H., Piggott S.J., Patterson Fife J. 2006. Application technology and environmental considerations for the use of entomopathogenic nematodes in biological control. Biol. Control 38, 124–133.
• Spiridonov S.E., Reid A.P., Podrucka K., Subbotin S.A., Moens M. 2004. Phylogenetic relationships within the genus Steinernema (Nematoda: Rhabditida) as inferred from analyses of sequences of ITS1-5.8S-ITS2 region of the rDNA and morphological features. Nematology 6(4), 547–566.
• Stock S.P., Campbell J.F., Nadler S.A. 2001. Phylogeny of Steinernema Travassos, 1927 (Cephalobina: Steinernematidae) inferred from ribosomal DNA sequences and morphological characters. J. Parasitol. 87(4), 877–889.
• Sturhan D. 1996. Studies on the natural occurrence and distribution of entomopathogenic nematodes. Russ J. Nematol 4, 98.
• Sturhan D. 1999. Prevalence and habitat specificity of entomopathogenic nematodes in Germany. Proceeding of a workshop held at Todi, Perugia, Italy, 16 to 29 May, 1995. Luxembourg: European Commission, COST 819, 123–132.
• Sturhan D., Lišková M. 1998. Studies on the natural occurrence of entomopathogenic nematodes in the Slovak Republic. Nematologica 44(5), 586.
• Sturhan D., Mráček Z. 2001. Records of the Steinernema glaseri groups (Nematoda: Rhabditida) in Europe, with notes on the morphological characteristics of this species group. Abstract, SIP 34th Annual Meeting, Noordwijkerhout, The Netherlands, August 25–30, 2001, 52–53.
• Sturhan D., Spiridonov S.E., Mráček Z. 2005. Steinernema silvaticum sp. N. (Rhabditida: Steinernematidae), a new entomopathocenic nematode from Europe. Nematology 7(2), 227–241.
• Subbotin S.A., Sturhan D., Vovlas N., Castillo P., Tanyi Tambee J., Moens M., Baldwin J.G. 2007. Application of the secondary structure model of rRNA for phylogeny: D2–D3 expansion segments of the LSU gene of plant-parasitic nematodes from the family Hoplolaimidae Filipjev, 1934. Mol.Phylogenet. Evol. 443, 881–890.