Decrease of RGCs number in glaucoma model vs. optic nerve transaction in rats

• Autorzy: Pietrucha-Dutczak M. , Smedowski A. , Wylegala E. , Lewin-Kowalik J.
• Języki publikacji: EN

Abstrakty

EN

Introduction: Transection of the optic nerve and glaucoma causes both structural and functional damage to retinal ganglion cells with subsequent vision defect or loss. This study was undertaken to compare the lost of RGCs after optic nerve transection and glaucoma model. Materials and methods: Wistar rats were divided into two groups. The first group underwent bilateral stereotactic injection of fluorescent tracer – Fluorogold (FG) into the superior colliculus to label RGCs. After one week the right optic nerve was transected. Left eye without optic nerve axotomy was established as control. In the second group intraocular pressure (right eye) was elevated by injection of polystyrene microbeads into anterior chamber (Bead model) and measured by Icare TonoLab. RGCs were labeled by FG before euthanasia. Fourteenth days following optic nerve transection and intraocular pressure elevation the total number of FG-positive RGCs was counted in seven radial sections through the optic disk. Results and conclusions: After axotomy the number of surviving cells was reduced to 20.2 % (from 2249.5 ± 127.2 – in control group to 454.7 ± 96.5 – in group after axotomy), in glaucoma model to 79.9% (from 2249.5 ± 127.2 – in control group to 1798.3 ± 118.96 – in glaucoma model).

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2013
• Tom: 73
• Numer: Suppl.1
• Opis fizyczny: p.56-57

Twórcy

autor

Pietrucha-Dutczak M.

• Department of Physiology, Medical University of Silesia, Katowice, Poland

autor

Smedowski A.

• Department of Physiology, Medical University of Silesia, Katowice, Poland
• Ophthalmology Clinic, Medical University of Silesia, Railway Hospital in Katowice, Katowice, Poland

autor

Wylegala E.

• Ophthalmology Clinic, Medical University of Silesia, Railway Hospital in Katowice, Katowice, Poland

autor

Lewin-Kowalik J.

• Department of Physiology, Medical University of Silesia, Katowice, Poland