Effect of increased copper ion content in the medium on the regeneration of androgenetic embryos of carrot (Daucus carota L.)

• Autorzy: Kowalska U. , Szafranska K. , Krzyzanowska D. , Kiszczak W. , Gorecki R. , Janas K. , Gorecka K.

Warianty tytułu

PL

Wpływ podwyższonej zawartości jonów miedzi w pożywce na regenerację zarodków androgenetycznych marchwi (Daucus carota L.)

• Języki publikacji: EN

Abstrakty

EN

The study was conducted to determine the effect of elevated concentrations of copper in the medium on the regeneration of androgenetic embryos of the carrot cultivar ‘Kazan F1’ obtained in anther cultures and to determine the level of soluble phenols produced in the regenerates under copper stress. Green embryos were laid out on 4 regeneration media based on B5 medium (G a m b o r g et al. 1968) without hormones, containing 0.1 – control, 1, 10, and 100 μM CuSO4×5H2O. The plant material was passaged 3 times, after 4, 9 and 15 weeks. During these passages the emerging structures were examined; they were classified in terms of growth and development in vitro, weighed and counted. The levels of soluble phenols in the freeze-dried regenerates were determined. The elevated concentrations of copper in the regeneration media affected positively the formation of complete plants (rooted rosettes) and secondary embryos during the first 4 weeks of culture. After a longer regeneration time (9, 15 weeks), the elevated concentrations of copper caused negative effects: deformation of rosettes. After 15 weeks, the number of rooted rosettes decreased. The 9-week culture subjected to copper stress brought about an increase in the amounts of soluble phenols. The highest values were recorded in the rosettes treated with 10 μM CuSO4. Prolonged exposure to media containing elevated concentrations of CuSO4 caused a reduction in the accumulation of phenolic compounds in the rosettes.

PL

Badania przeprowadzono w celu określenia wpływu wyższych stężeń miedzi w pożywce na proces regeneracji zarodków androgenetycznych marchwi odmiany ‘Kazan F1’ uzyskanych w kulturach pylnikowych oraz określenia poziomu rozpuszczalnych fenoli powstałych w regenerantach pod wpływem stresu miedzi. Zazielenione zarodki wykładano na 4 pożywki regeneracyjne B5 (G a m b o r g i in. 1968) bez hormonów zawierających 0,1 – kontrola; 1; 10; i 100 μM CuSO4×5H2O. Materiał roślinny przepasażowano 3-krotnie po 4, 9 i 15 tygodniach. Podczas tych pasaży obserwowano powstające struktury, które odpowiednio sklasyfikowano pod względem wzrostu i rozwoju w kulturze in vitro. Analizowano liczbę i masę otrzymanych struktur. W zliofilizowanych regenerantach określono poziom rozpuszczalnych fenoli. Podwyższone stężenia miedzi w pożywkach regeneracyjnych wpływały korzystnie na powstawanie kompletnych roślin (ukorzenionych rozet) i wtórnych zarodków w trakcie pierwszych 4 tygodni kultury. Po dłuższym czasie regeneracji (9,15 tygodni) wyższe stężenia miedzi wywoływały negatywne efekty: deformacje rozet. Po 15 tygodniach spadała liczba ukorzenionych rozet. 9-tygodniowa kultura poddana stresowi miedzi wywołała wzrost zawartości fenoli rozpuszczalnych. Najwyższe wartości odnotowano w rozetach traktowanych 10μM CuSO4. Długotrwała ekspozycja na pożywkach zawierających podwyższone stężenia CuSO4 powodowała obniżenie akumulacji związków fenolowych w rozetach.

• Wydawca: -
• Czasopismo: Acta Agrobotanica
• Rocznik: 2012
• Tom: 65
• Numer: 2
• Opis fizyczny: p.73-81,fig.,ref.

Twórcy

autor

Kowalska U.

• Department of Vegetable Crops, Institute of Horticulture, Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland

autor

Szafranska K.

autor

Krzyzanowska D.

autor

Kiszczak W.

autor

Gorecki R.

autor

Janas K.

autor

Gorecka K.

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