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2015 | 71 | 08 |
Tytuł artykułu

First molecular identification of Spironucleus salmonis (Diplomonadida) from diseased rainbow trout Oncorchynchus mykiss in Poland

Autorzy
Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The aim of this study was the isolation of diplomonads from the digestive tract of infected rainbow trout (Oncorhynchus mykiss) and the molecular identification of Spironucleus species from samples. Samples were collected from a total of 40 fish (0.8 g ± 0.1) from a commercial farm in Pomorskie Voivodeship, Poland. Polymerase chain reaction (PCR) and a partial sequence analysis of the 18S ribosomal gene were used to identify Spironucleus species. The PCR of the 18S rDNA yielded a 705 bp DNA band on agarose gel, and a sequence analysis of the DNA confirmed the isolate as Spironucleus salmonis. This is the first molecular identification of an isolate of S. salmonis in Poland. Further studies are needed to determine the prevalence of the parasite in this species of fish in other locations and to investigate the impact of the parasite on the total fish population.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
71
Numer
08
Opis fizyczny
p.497-499,fig.,ref.
Twórcy
autor
  • Institute of Biological Bases of Animal Diseases, Sub-department of Fish Diseases and Biology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland
autor
  • Institute of Biological Bases of Animal Diseases, Sub-department of Fish Diseases and Biology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland
Bibliografia
  • 1. Bassleer G.: Disease prevention and control. Spironucleus/Hexamita infection, hole-in-the-head disease. Freshw. Mar. Aquar. 1983, 6, 38-60.
  • 2. Davis H. S.: Octomitus salmonis, a parasitic flagellate of trout. Bull. US Bur. Fish. 1926, 42, 9-26.
  • 3. Einszporn-Orecka T.: Flagellates Spironucleus anguillae sp. n. parasites of eel (Anguilla anguilla L.). Acta Parasitol. 1979, 18, 237-242.
  • 4. Fard M. R. S., Jørgensen A., Sterud E., Bleiss W., Poynton S. L.: Ultrastructure and molecular diagnosis of Spironucleus salmonis (Diplomonadida) from rainbow trout Oncorhynchus mykiss in Germany. Dis. Aquat. Org. 2007, 75, 37-50.
  • 5. Guo F. C., Woo P. T.: Detection and quantification of Spironucleus barkhanus in experimentally infected Atlantic salmon, Salmo salar. Dis. Aquat. Org. 2004, 61, 175-178.
  • 6. Guo F. C., Woo P. T.: Experimental infections of Atlantic salmon Salmo salar with Spironucleus barkhanus. Dis. Aquat. Org. 2004, 61, 59-66.
  • 7. Jorgensen A., Sterud D.: The marine pathogenic genotype of Spironucleus barkhanus from farmed salmonids redescribed as Spironucleus salmonicida n. sp. J. Eukaryot. Microbiol. 2006, 53, 531-541.
  • 8. Kent M. L., Ellis J., Fournie J. W., Dawe S. C., Bagshaw J. W., Whitaker D. J.: Systemic hexamitid (Protozoa: Diplomonadida) infection in seawater penreared Chinook salmon Oncorhynchus tshawytscha. Dis. Aquat. Org. 1992, 14, 81-89.
  • 9. Kulda J., Lom J.: Remarks on the diplomastigine flagellates from the intestine of fishes. Parasitology 1964, 54, 753-762.
  • 10. L82/14 CRE (1998) Veterinary drug residues: regulatory issues.
  • 11. Mo T. A., Poppe T. T., Iversen L.: Systemic hexamitosis in salt-water reared Atlantic salmon (Salmo salar L.). Bull. Eur. Assoc. Fish Pathol. 1990, 10, 69-70.
  • 12. Moore E.: Octomitus salmonis, a new species of intestinal parasite in trout. Trans. Am. Fish. Soc. 1922, 52, 74-97.
  • 13. Poppe T. T., Mo T. A.: Systemic, graulomatous hexamitosis of farmed Atlantic salmon: interaction with wild fish. Fish. Res. 1993, 17, 147-152.
  • 14. Poppe T. T., Mo T. A., Iversen L.: Disseminated hexamitosis in sea-caged Atlantic salmon Salmo salar. Dis. Aquat. Org. 1992, 14, 91-97.
  • 15. Poynton S. L., Fard R. S., Jenkins J., Ferguson H. W.: Ultrastructure of Spironucleus salmonis n. comb. (formerly Octomitus salmonis sensu Moore 1922, Davis 1926, and Hexamita salmonis sensu Ferguson 1979), with a guide to Spironucleus species. Dis. Aquat. Org. 2004, 60, 49-64.
  • 16. Poynton S. L., Sterud E.: Guidelines for species descriptions of diplomonad flagellates from fish. J. Fish Dis. 2002, 25, 15-31.
  • 17. Sangmaneedet S., Smith S. A.: Efficacy of various chemotherapeutic agents on the growth of Spironucleus vortens, an intestinal parasite of the freshwater angelfish. Dis. Aquat. Org. 1999, 38, 47-52.
  • 18. Sterud E., Mo T. A., Poppe T. T.: Systemic spironucleosis in sea-farmed Atlantic salmon Salmo salar, caused by Spironucleus barkhanus transmitted from feral Arctic char Salvelinus alpinus? Dis. Aquat. Org. 1998, 33, 63-66.
  • 19. Tamura K., Dudley J., Nei M., Kumar S.: MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 2007, 24, 1596-1599.
  • 20. Thompson J. D., Higgins D. G., Gibson T. J.: Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl. Acids Res. 1994, 22, 4673-4680.
  • 21. Tojo J. L., Santamarina M. T.: Oral pharmacological treatments for parasitic diseases of rainbow trout Oncorhynchus mykiss. I: Hexamita salmonis. Dis. Aquat. Org. 1998, 33, 51-56.
  • 22. Uldal A.: Life cycle observations on Hexamita salmonis from rainbow trout intestine. In vitro studies. Bull. Eur. Ass. Fish Pathol. 1996, 16, 112-114.
  • 23. Wierzbicka J., Orecka-Grabda T.: Protozoans parasitic on Anguilla anguilla (L.) from the Szczecin Lagoon and River Odra mouth. Acta Ichthyol. Piscat. 1994, 24, 3-11.
  • 24. Wierzbicka J., Sobecka E.: Parasitic fauna of burbot, Lota lota (L.) in the lake Dębie. Acta Ichthyol. Piscat. 1985, 15, 191-202.
  • 25. Williams C. F., Lloyd D., Poynton S. L., Jorgensen A., Millet C. O. M., Cable J.: Spironucleus species: Economically-important fish pathogens and enigmatic single-celled eukaryotes. J. Aquac. Res. Development 2011, S2:002. doi:10.4172/2155- 9546.S2-002.
  • 26. Williams C. F., Vacca A. R., Lloyd D., Schelkle B., Cable J.: Non-invasive investigation of Spironucleus vortens transmission in freshwater angelfish Pterophyllum scalare. Dis. Aquat. Org. 2013, 105, 211-223.
Typ dokumentu
Bibliografia
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Identyfikator YADDA
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