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2015 | 18 | 4 |
Tytuł artykułu

Expression of goose parvovirus whole VP3 protein and its epitopes in Escherichia coli cells

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The aim of this study was the expression of goose parvovirus capsid protein (VP3) and its epitopes in Escherichia coli cells. Expression of the whole VP3 protein provided an insufficient amount of protein. In contrast, the expression of two VP3 epitopes (VP3ep4, VP3ep6) in E. coli, resulted in very high expression levels. This may suggest that smaller parts of the GPV antigenic determinants are more efficiently expressed than the complete VP3 gene.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
18
Numer
4
Opis fizyczny
p.879-880,ref.
Twórcy
autor
  • Department of Poultry Viral Diseases, National Veterinary Research Institute, Partyzantów 57, 24-100 Pulawy, Poland
  • Department of Poultry Viral Diseases, National Veterinary Research Institute, Partyzantów 57, 24-100 Pulawy, Poland
  • Department of Microbiology, Gdansk University of Technology, Gabriela Narutowicza 11/12, 80-233 Gdansk, Poland
Bibliografia
  • Derzsy D (1967) A viral disease of goslings. I. Epidemiological, clinical, pathological and aetiological studies. Acta Vet Acad Sci Hung 17: 443-448.
  • Fournier D, Gaudry D (1992) Recent discoveries on waterfowl pathology: a new parvovirus of Muscovy ducks in France-Field vaccination trials. In: McNulty MS, McFerran JB (eds.) Proceedings of the Commission of the European Communities Meeting on Virus Diseases of Poultry-New and Evolving Pathogens. Brussels, Belgium, pp 183-194.
  • Ju H, Wei N, Wang Q, Wang C, Jing Z, Guo L, Liu D, Gao M, Ma B, Wang J (2011) Goose parvovirus structural proteins expressed by recombinant baculoviruses self-assemble into virus-like particles with strong immunogenicity in goose. Biochem Biophys Res Commun 409: 131-136.
  • Kisary J, Derzsy D, Meszaros J (1978) Attenuation of the goose parvovirus strain B. Laboratory and field trials of the attenuated mutant for vaccination against Derzsy’s disease. Avian Pathol 7: 397-406.
  • Le Gall-Recule G, Jestin V, Chagnaud P, Blanchard P, Jestin A (1996) Expression of muscovy duck parvovirus capsid proteins (VP2 and VP3) in a baculovirus expression system and demonstration of immunity induced by the recombinant proteins. J Gen Virol 77: 2159-2163.
  • Tarasiuk K, Woźniakowski G, Samorek-Salamonowicz E (2013) Loop-mediated isothermal amplification as a simple molecular method for the detection of Derzsy’s disease virus. Bull Vet Inst Pulawy 57: 19-23.
  • Yu T-F, Ma B, Gao M-C, Wang J-W (2012) Localization of linear B-cell epitopes on goose parvovirus structural protein. Vet Immunol Immunopathol 145: 522-526.
  • Zhang Y, Li Y, Liu M, Zhang D, Guo D, Liu C, Zhi H, Wang X, Li G, Li N, Liu S, Xiang W, Tong G (2010) Development and evaluation of a VP3-ELISA for the detection of goose and Muscovy duck parvovirus antibodies. J Virol Methods 163: 405-409.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
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