Wpływ 2-metylo-6-(fenyloetylo)-pirydyny - MPEP - selektywnego antagonisty receptora mGluR5 na zachowanie szczurów w teście otwartego pola w sytuacji oczekiwania na nieunikniony stres

• Autorzy: Nawrocka M. , Kowalczyk M.

Warianty tytułu

EN

Effects of 2-methyl-6-(phenylethyl)-pyridine (MPEP) – a selective mGluR5 receptor antagonist – on the behaviour of rats in an open field test in a situation of predicted inevitable stress

• Języki publikacji: PL

Abstrakty

EN

The aim of the work was to assess the effects of MPEP - a selective mGluR5 receptor antagonist with anti-anxiety properties - on the behaviour of rats in a situation of predicted and inevitable stress. Wistar rats kept under a reversed light-dark cycle (the light phase starting at 8 pm) were subjected to a stress procedure including immobilisation for two hours a day during five subsequent days. Prior to the first stress, tests were performed to evaluate general motor activity and activity in the central field (a fear response). On subsequent days the tests were repeated immediately before immobilisation at 24-hour intervals. After the last immobilisation, all animals were moved to their original cages. After a 48-hour break, the animals were divided into two groups: a control group, which received 0.9% NaCl (1 mL/kg i.p.), and a test group, which received MPEP (10 mg/1mL/kg i.p.) Sixty minutes after injections, examinations preceding the expected stress were repeated in the control and test groups. A four-day stress significantly reduced the total number of passages between fields and the activity in the central field compared with the situation prior to stress. A 48-hour break after the last stress situation resulted in a significant increase in general locomotor activity and activity in the central field in the control group. In the MPEP group, general locomotor activity was significantly reduced compared with the control group, and there was also an increased proportion of passages in the central field by comparison with the control group and with the baseline prior to a stress situation. The results of this study indicate that MPEP - an mGluR5 receptor antagonist with anti-anxiety properties - may prevent psychomotor excitation caused by a situation of predicted stress and may have anti-stress properties.

Słowa kluczowe

PL

szczury; zachowanie sie zwierzat; stresy; oczekiwanie na nieunikniony stres; antagonisci receptorow metabotropowych glutaminianergicznych; 2-metylo-6-(fenyloetylo)-pirydyna; wlasciwosci przeciwlekowe; reakcja na stres; aktywnosc motoryczna; test otwartego pola

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2012
• Tom: 68
• Numer: 06
• Opis fizyczny: s.376-379,rys.,bibliogr.

Twórcy

autor

Nawrocka M.

• Zakład Farmakologii i Toksykologii, Wojskowy Instytut Higieny i Epidemiologii, ul.Kozielska 4, 01-163 Warszawa

autor

Kowalczyk M.

Bibliografia

• 1.Brandão M. L., Zanoveli J. M., Ruiz-Martinez R. C., Oliveira L. C., Landeira-Fernandez J.: Different patterns of freezing behavior organized in the periaqueductal gray of rats: Association with different types of anxiety. Behav Brain Res. 2008, 188, 1-13.
• 2.Brodkin J., Busse C., Sukoff S. J., Varney M. A.: Anxiolytic-like activity of the mGluR5 antagonist MPEP. A comparison with diazepam and buspirone. Pharmacol. Biochem. Behav. 2002, 73, 359-366.
• 3.Busse C. S., Brodkin J., Tattersall D., Anderson J. J., Warren N., Tehrani L., Bristow L. J., Varney M. A., Cosford N. D. P.: The behavioral profile of the potent and selective mGlu5 receptor antagonist 3-[(2-methyl-1,3-thiazol-4-yl)ethynyl]pyridine (MTEP) in rodent models of anxiety. Neuropsychopharmacol. 2004, 29, 1971-1979.
• 4.Chaouloff F., Hémar A., Manzoni O.: Acute stress facilitates hippocampal CA1 metabotropic glutamate receptor-dependent long-term depression. J. Neurosci. 2007, 27, 7130-7135.
• 5.Cordero M. I., Kruyt N. D., Sandi C.: Modulation of contextual fear conditioning by chronic stress in rats is related to individual differences in behavioral reactivity to novelty. Brain Res. 2003, 970, 242-245.
• 6.Cortese B. M., Phan K. L.: The role of glutamate in anxiety and related disorders. CNS Spectrums. 2005, 10, 820-830.
• 7.Frohardt R. J., Guarraci F. A., Young S. L.: Intrahippocampal infusions of a metabotropic glutamate receptor antagonist block the memory of context-specific but not tone-specific conditioned fear. Behav. Neurosci. 1999, 113, 222-227.
• 8.Grønli J.: Chronic mild stress – an animal model of depression, from behavior to molecules. Praca dokt., Department of Biomedicine, Section of Physiology, University of Bergen, Norway 2006.
• 9.Kabbaj M., Devine D. P., Savage V. R., Akil H.: Neurobiological Correlates of Individual Differences in Novelty-Seeking Behavior in the Rat: Differential Expression of Stress-Related Molecules. J. Neurosci. 2000, 20, 6983-6988.
• 10.Mendl M., Burman O. H. P., Paul E. S.: An integrative and functional framework for the study of animal emotion and mood. Proc. R. Soc. B 2010, 277, 2895-2904.
• 11.O'Keefe J., Nadel L.: Aversively motivated behaviour in: The Hippocampus as a Cognitive Map. Oxford University Press 1978.
• 12.Pijlman F. T. A., van Ree J. M.: Physical but not emotional stress induces a delay in behavioural coping responses in rats. Behav Brain Res. 2002, 136, 365-373.
• 13.Ruden R. A.: A model for disrupting an encoded traumatic memory. Traumatology 2007, 13, 71-75.
• 14.Satow A., Maehara S., Ise S., Hikichi H., Fukushima M., Suzuki G., Kimura T., Tanaka T., Ito S., Kawamoto H., Ohta H.: Pharmacological effects of the metabotropic glutamate receptor 1 antagonist compared with those of the metabotropic glutamate receptor 5 antagonist and metabotropic glutamate receptor 2/3 agonist in rodents: detailed investigations with a selective allosteric metabotropic glutamate receptor 1 antagonist, FTIDC [4-[1-(2-fluoropyridine-3-yl)-5-methyl-1H-1,2,3-triazol-4-yl]-N-isopropyl-N-methyl-3,6-dihydropyridine-1(2H)-carboxamide]. J. Pharmacol. Exp. Ther. 2008, 326, 577-586.
• 15.Schwendt M., Jezova D.: Gene expression of NMDA receptor subunits in rat adrenals under basal and stress conditions. J. Physiol. Pharmacol. 2001, 52, 719-727.
• 16.Spooren W. P. J. M., Vassout A., Neijt H. C., Kuhn R., Gasparini F., Roux S., Porsolt R. D., Gentsch C.: Anxiolytic-like effects of the prototypical metabotropic glutamate receptor 5 antagonist 2-methyl-6-(phenylethynyl)pyridine in rodents. J. Pharmacol. Exp. Therap. 2000, 295, 1267-1275.
• 17.Sudakov K. V., Yumatov E. A., Tarakanov O. P.: The Integral Criterion of Emotional Stress: Int. J. Stress Manage 1996, 3, 37-46.
• 18.Tatarczyńska E., Kłodzińska A., Chojnacka-Wójcik E., Pałucha A., Gasparini F, Kuhn R., Pilc A.: Potential anxiolytic- and antidepressant-like effects of MPEP, a potent, selective and systemically active mGlu5 receptor antagonist. Brit. J. Pharmacol. 2001, 132, 1423-1430.
• 19.Yumatov E. A., Kuz'menko V. A., Badikov V. I., Glazachev O. S., Ivanova L. I.: Emotional Stress in Students during Examinations. Human Physiol. 2001, Vol. 27, 221-227.