Wpływ kwasów huminowych w dawkach dla owiec na aktywność enzymatyczną żwacza

• Autorzy: Majewska M.P. , Miltko R. , Skomial J. , Kowalik B.

Warianty tytułu

EN

Influence of humic acid supplemented to sheep diets on rumen enzymatic activity

• Języki publikacji: PL

Abstrakty

EN

The objective of the present study was to investigate how different levels of natural humic acid in the diet affect the hydrolases activity in the rumen of sheep. The experiment was carried out on three rumen-fistulated adult sheep. The control animals were fed a diet (K) containing 59.7% meadow hay, 28.4% crushed barley, 9.8% soybean oil meal and 2.1% vitamin mineral mixture. The experimental diets were composed of the control ration supplemented with humic acid (HUMAC natur AFM® containing 65% humic acid in dry matter) and supplied at doses of 10 g · d⁻¹ (diet H10) or 20 g · d⁻¹ (diet H20). Samples of rumen digesta were collected just before the morning feeding, as well as 2, 4, and 8 h afterwards. In the present study, the cellulolytic activity in the rumen digesta was higher (P < 0.01) in sheep fed the K diet than it was in animals fed the H20 ration 2 h after feeding. However, the effect of humic acid on the cellulose degradation rate depended on the content of humic acid in the diet. The cellulolytic activity was increased 2 h after feeding in sheep fed the H10 diet as compared with animals fed the H20 ration (P < 0.05). Sampling time had significant influence on the hydrolases activity in the rumen of sheep. In sheep fed the H10 ration, the cellulolytic activity was higher 8 h after feeding than it was before feeding and 4 h after feeding (P < 0.05). In the H10 and H20 groups, the xylnalolytic activity was higher before feeding, but lower than it was in the control group K. The degradation rates of xylan were decreased 2 and 4 h after feeding in sheep fed the H10 and H20 rations and then increased 8 h after feeding (P < 0.05). In sheep fed the H20 diet, the amylolitic activity 8 h after feeding was higher than it was 2 h after feeding (P < 0.05). The effect of humic acid on the hydrolases activity in the rumen of sheep is not uniform and depends on the quantity of humic acid in the diet and on sampling time. Humic acid present in commercial HUMAC natur AFM® product enhances the initial rate of cellulose degradation in the rumen at a dose of 10 g · d⁻¹. Thus, it may suggest that humic acid stimulates the growth of fibrolytic microorganisms in the rumen. On the other hand, the present results suggest that humic acid may reduce the rate of carbohydrate degradation in the rumen, since a higher hydrolases activity was observed before morning feeding and 8 h afterwards.

Słowa kluczowe

PL

owce; zywienie zwierzat; dodatki paszowe; kwasy huminowe; zwacz; tresc zwacza; enzymy hydrolityczne; aktywnosc enzymatyczna

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2017
• Tom: 73
• Numer: 12
• Opis fizyczny: s.770-773,tab.,bibliogr.

Twórcy

autor

Majewska M.P.

• Instytut Fizjologii i Żywienia Zwierząt im. Jana Kielanowskiego, Polska Akademia Nauk, ul. Instytucka 3, 05-110 Jabłonna

autor

Miltko R.

• Instytut Fizjologii i Żywienia Zwierząt im. Jana Kielanowskiego, Polska Akademia Nauk, ul. Instytucka 3, 05-110 Jabłonna

autor

Skomial J.

• Instytut Fizjologii i Żywienia Zwierząt im. Jana Kielanowskiego, Polska Akademia Nauk, ul. Instytucka 3, 05-110 Jabłonna

autor

Kowalik B.

• Instytut Fizjologii i Żywienia Zwierząt im. Jana Kielanowskiego, Polska Akademia Nauk, ul. Instytucka 3, 05-110 Jabłonna

Bibliografia

• AOAC. Association of Official Analytical Chemists, Official Methods of Analysis. Arlington, VA 2011.
• Faulkenbery J. M.: Effect of humic acid on forage digestibility in ruminants. Master’s thesis, Texas A&M University 1995.
• Galip N., Polat U., Bircik H.: Effect of supplemental humic acid on ruminal fermentation and blood variables in rams. Ital. J. Anim. Sci. 2016, 74, 390-393.
• Huhtanen P. P., Khalili H.: The effect of sucrose supplement on the particle-associated carboxymethylcellulase (EC 3.2.1.4) and xylanase (EC 3.2.1.8) activities in cattle given grass-sillage-based diet. Brit. J. Nutr. 1992, 67, 245-255.
• Kowalik B., Skomiał J., Pająk J. J., Taciak M., Majewska M., Bełżecki G.: Population of ciliates, rumen fermentation, indicators and biochemical parameters of blood serum in heifers fed diets supplemented with yeast (Saccharomyces cerevisiae) preparation. Anim. Sci. Pap. Rep. 2012, 30, 329-338.
• Islam K. M. S., Schuhmacher A., Groop J. M.: Humic acid substances in animal agriculture. Pak. J. Nutr. 2005, 4, 126-134.
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• Miltko R., Bełżecki G., Kowalik B., Skomiał J.: Presence of carbohydrate-digesting enzymes throughout the digestive tract of sheep. Turk. Vet. Anim. Sci. 2016, 40, 271-277.
• StatSoft, Inc. STATISTICA. Data analysis software system, ver. 10. www. statsoft.com, 2011.
• Thomassen B. P. H., Faust R. H.: The use of a processed humic acid product as a feed supplement in dairy production in the Netherlands. The World Grow Organic International Scientific Conference, August, Basle 2000, s. 339.
• Váradyová Z., Kišidayová S., Jalč D.: Effect of humic acid on fermentation and ciliate protozoan population in rumen fluid of sheep in vitro. J. Sci. Food Agric. 2009, 89, 1936-1941.
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• Yörük M. A., Gül M., Hayirli A., Macit M.: The effects of supplementation of humate and probiotic on egg production and quality parameters during the late laying period in hens. Poult. Sci. Assoc. 2004, 83, 84-88.

Identyfikatory

DOI

10.21521/mw.5822