Screening of medium components and process parameters for sugar beet molasses vinasse decolorization by Lactobacillus plantarum using plackett-burman experimental design

• Autorzy: Seruga P. , Krzywonos M.
• Języki publikacji: EN

Abstrakty

EN

Sugar beet molasses vinasse is characterized by a high COD and dark brown color produced by three groups of colorants: melanoidins, caramels, and invert degradation products of alkaline hydrolysis. These environmental pollutants have to be effectively removed via biodegradation processes before discharge into a watercourse. The aim of this study was to assess how the type of the bacterial culture used (static or agitated), and the addition of nutrients (wheat stillage, MgSO₄·7H₂O, (NH₄)₂SO₄, KH₂PO₄, yeast extract, glucose, fructose, sucrose, and peptone) contribute to the effectiveness of sugar beet molasses vinasse decolorization with Lactobacillus plantarum. The Plackett-Burman experimental design was used to reduce the number of experiments. Decolorization of nutrient-enriched vinasse (25% v/v) was performed at an initial pH of 6.25 and 37ºC for 7 days. Color was measured spectrophotometrically at 475 nm. Concentrations of sugars, organic acids, and colorants were determined by HPLC. The nutrient was screened when the probability value exceeded 95% of the confidence level. 67% efficiency of decolorization of sugar beet molasses vinasse by L. plantarum was obtained. The type of bacterial culture and the addition of wheat stillage, yeast extract, glucose, peptone, and (NH₄)₂SO₄ were significant factors for the decolorization process.

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2015
• Tom: 24
• Numer: 2
• Opis fizyczny: p.683-688,fig.,ref.

Twórcy

autor

Seruga P.

• Department of Bioprocess Engineering, Wroclaw University of Economics, Komandorska 118/120, 53-345 Wroclaw, Poland

autor

Krzywonos M.

• Department of Bioprocess Engineering, Wroclaw University of Economics, Komandorska 118/120, 53-345 Wroclaw, Poland

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Identyfikatory

DOI

10.15244/pjoes/24931