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2011 | 41 | 4 |
Tytuł artykułu

Screening assessment of cyanobacterial embryotoxicity to Japanese medaka, Oryzias latipes (Actinopterygii: Beloniformes: Adrianichthyidae)

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Warianty tytułu
Języki publikacji
EN
Abstrakty
Background. Fish embryos and larvae are frequently subject to chronic- and damaging exposure of cyanotoxins released by live and decomposing cyanobacteria Whereas the majority of former studies dealt with the embryotoxicological effects of pure toxins and extracts, we focused on the evaluation of toxic effects of crude cyanobacterial biomass in our study. Materials and methods. Samples of crude cyanobacterial biomass—intended for embryotoxicological tests with eggs of Japanese medaka, Oryzias latipes (strain Q2d-rR.YHNI)—were collected from natural bodies of water in the Czech Republic. Those samples consisted predominantly of: Microcystis aeruginosa, M. flos-aquae, Woronichinia naegeliana, Anabaena sigmoidea, and Aphanizomenon flos-aquae. In the preliminary optimization experiments, various numbers of fertilized medaka eggs (from 1 to 6 per one 10-mL well on a six well polypropylene plate) were hatched under standard conditions. Because of the highest hatching rates, 4 eggs per 10 mL well were selected as an optimal treatment. The embryotoxicological tests were performed according to the OECD 212 guideline in two concentrations (extracts of crude cyanobacterial biomass: 40 and 200 mg · L–1dry weight). Fertilized eggs in stage 6 to 8 (some 2–3 h after fertilization) were introduced into test wells, and hatching performance, duration of embryonic development as well as lethal and sublethal effects were monitored. Results. The hatching rates (treatment with 4 eggs per 10 mL well), achieved in the majority of experimental treatments with cyanobacterial biomass, were significantly different from the control group. Also hatching onset was considerably delayed due the presence of cyanobacterial biomass. The embryotoxicological impacts were more pronounced in higher concentrations of cyanobacterial biomass (200 mg · L–1) compared to lower concentrations (40 mg · L–1). Whilst deformities were not recorded in the control, their highest incidence amounted to 11.8% and 40.9% in 40 and 200 mg · L–1 of cyanobacterial biomass, respectively. Conclusion. Cyanotoxicity significantly affected examined parameters (hatching rates, duration of embryonic development, and morphological deformities occurrence) in medaka embryos. The study proved significant embryotoxic effects upon Japanese medaka at environmentally relevant levels of cyanobacteria commonly occurring in surface waters in Europe.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
41
Numer
4
Opis fizyczny
p.293–299,ref.
Twórcy
autor
  • Faculty of Fisheries and Protection of Waters, South Bohemian Research Center for Aquaculture and Biodiversity of Hydrocenoses and Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic
autor
  • Faculty of Fisheries and Protection of Waters, South Bohemian Research Center for Aquaculture and Biodiversity of Hydrocenoses and Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic
autor
  • Faculty of Science, Research Centre for Toxic Compounds in the Environment (RECETOX), Masaryk University, Kamenice 3, 62500 Brno, Czech Republic
autor
  • Department of Experimental Phycology and Ecotoxicology, Institute of Botany, Czech Academy of Sciences, Lidicka 25/27, 657 00 Brno, Czech Republic
Bibliografia
  • Anonymous 1998. OECD Guideline for testing of chemicals—fish, short-term toxicity test on embryo and sac-fry stages. OECD 212.
  • Anonymous 1999a. ČSN EN ISO 7346-1 (757761). Jakost vod – Stanovení akutní letální toxicity látek pro sladkovodní ryby [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] – Část 1: Statická metoda. {Water quality—Determination of the acute lethal toxicity of substances to freshwater fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)]. Part 1: Static method.} CNI, Prague, Czech Republic. [In Czech.]
  • Anonymous 1999b. ČSN EN ISO 7346-2 (757761). Jakost vod – Stanovení akutní letální toxicity látek pro sladkovodní ryby [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] – Část 2: Obnovovací metoda. {Water quality-Determination of the acute lethal toxicity of substances to freshwater fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)]. Part 2: Renewal method.} CNI, Prague, Czech Republic. [In Czech.]
  • Babica P., Kohoutek J., Bláha L., Adamovský O., Maršálek B. 2006. Evaluation of extraction approaches linked to ELISA and HPLC for analyses of microcystin-LR, -RR and -YR in freshwater sediments with different organic material content. Analytical and Bioanalytical Chemistry 385 (8): 1545–1551. DOI: 10.1007/s00216-006-0545-8
  • Berry J.P., Gantar M., Gibbs P.D.L., Schmale M.C. 2007. The zebrafish (Danio rerio) embryo as a model system for identification and characterization of developmental toxins from marine and freshwater microalgae. Comparative Biochemistry and Physiology C 145 (1): 61–72. DOI: 10.1016/j.cbpc.2006.07.011
  • Berry J.P., Gibbs P.D.L., Schmale M.C., Saker M.L. 2009. Toxicity of cylindrospermopsin, and other apparent metabolites from Cylindrospermopsis raciborskii and Aphanizomenon ovalisporum, to the zebrafish (Danio rerio) embryo. Toxicon 53 (2): 289–299. DOI: 10.1016/j.toxicon.2008.11.016
  • Bischoff K. 2001. The toxicology of microcystin-LR: Occurrence, toxicokinetics, toxicodynamics, diagnosis and treatment. Veterinary and Human Toxicology 43 (5): 294–297.
  • Burýšková B., Hilscherová K., Babica P., Vršková D., Maršálek B., Bláha L. 2006. Toxicity of complex cyanobacterial samples and their fractions in Xenopus laevis embryos and the role of microcystins. Aquatic Toxicology 80 (4): 346–354. DOI: 10.1016/j.aquatox.2006.10.001
  • Djediat C., Malécot M., de Luze A., Bernard C., Puiseux-Dao S., Edery M. 2010. Localization of microcystin-LR in medaka fish tissues after cyanotoxin gavage. Toxicon 55 (2–3): 531–535. DOI: 10.1016/j.toxicon.2009.10.005
  • Gregor J., Maršálek B. 2004. Freshwater phytoplankton quantification by chlorophyll a: a comparative study of in vitro, in vivo and in situ methods. Water Research 38 (3): 517–522. DOI: 10.1016/j.watres.2003.10.033
  • Helmstetter M.F., Maccubin A.E., Alden R.W.III. 1996. The medaka embryo-larval assay: an in vivo assay for toxicity, teratogenicity and carcinogenicity. Pp. 93–122. In: Ostrander G.K. (ed.) Techniques in aquatic toxicology. Lewis Publishers Boca Raton, New York, London, Tokyo.
  • Huynh-Delerme C., Edery M., Huet H., Puiseux-Dao S., Bernard C., Fontaine J.-J., Crespeau F., de Luze A. 2005. Microcystin-LR and embryo–larval development of medaka fish, Oryzias latipes. I. Effects on the digestive tract and associated systems. Toxicon 46 (1): 16–23. DOI: 10.1016/j.toxicon.2005.03.009
  • Jacoby J.M., Collier D.C., Welch E.B., Hardy F.J., Crayton M. 2000. Environmental factors associated with a toxic bloom of Microcystis aeruginosa. Canadian Journal of Fisheries and Aquatic Sciences 57 (1): 231–240. DOI: 10.1139/cjfas-57-1-231
  • Jacquet C., Thermes V., de Luze A., Puiseux-Dao S., Bernard C., Joly J.-S., Bourrat F., Edery M. 2004. Effects of microcystin-LR on development of medaka fish embryos (Oryzias latipes). Toxicon 43 (2): 141–147. DOI: 10.1016/j.toxicon.2003.11.010
  • Kinoshita M., Murata K., Naruse K., Tanaka M. 2009. Medaka. Biology, management, and experimental protocols. Wiley-Blackwell, Ames.
  • Lecoz N., Malécot M., Quiblier C., Puiseux-Dao S., Bernard C., Crespeau F., Edery M. 2008. Effects of cyanobacterial crude extracts from Planktothrix agardhii on embryo–larval development of medaka fish, Oryzias latipes. Toxicon 51 (2): 262–269. DOI: 10.1016/j.toxicon.2007.09.011
  • Liu Y., Song L., Li X., Liu T. 2002. Toxic effects of microcystin-LR on embryo larval and juvenile development of loach, Misguruns mizolepis Gunthe. Toxicon 40 (4): 395–399. DOI: 10.1016/S0041-0101(01)00173-8
  • Llewellyn G.C., Stephenson G.A., Hofman J.W. 1977. Aflatoxin B1 induced toxicity and teratogenicity in Japanese medaka eggs (Oryzias latipes). Toxicon 15 (6): 582–587. DOI: 10.1016/0041-0101(77)90111-8
  • Maršálek B., Bláha L. 2000. Microbiotests for cyanobacterial toxins screening. Pp. 519–526. In: Persoone G., Janssen C., De Coen W. (eds.) New microbiotests for routine toxicity screening and biomonitoring. Kluwer Academic/Plenum Publishers, New York, NY, USA.
  • Maršálek B., Bláha L., Hindák F. 2000. Review of toxicity of cyanobacteria in Slovakia. Biologia Bratislava 55 (6): 645–652.
  • Maršálek B., Bláha L., Turánek J. 2001. Microcystin LR and total microcystins in Czech reservoirs during 1993–1998. Pp. 55–62. In: Chorus I. (ed.) Cyanobacterial toxins. Springer Verlag, Berlin.
  • Oberemm A., Fastner J., Steinberg C.E.W. 1997. Effects of microcystin-LR and cyanobacterial crude extracts on embryo-larval development of zebrafish (Danio rerio). Water Research 31 (11): 2918–2921. DOI: 10.1016/S0043-1354(97)00120-6
  • Oberemm A., Becker J., Codd G.A., Steinberg C. 1999. Effects of cyanobacterial toxins and aqueous crude extracts of cyanobacteria on the development of fish and amphibians. Environmental Toxicology 14 (1): 77–87. DOI: 10.1002/(SICI)1522-7278(199902)14:1<77::AIDTOX11>3.0.CO;2-F
  • Palíková M., Navrátil S., Maršálek B., Bláha L. 2003. Toxicity of crude extract of cyanobacteria for embryos and larvae of carp (Cyprinus carpio L.). Acta Veterinaria Brno 72 (3): 437–443. DOI: 10.2754/avb200170040443
  • Palíková M., Krejčí R., Hilscherová K., Burýšková B., Babica P., Navrátil S., Kopp R., Bláha L. 2007a. Effects of different oxygen saturation on activity of complex biomass and aqueous crude extract of cyanobacteria during embryonal development in carp (Cyprinus carpio L.). Acta Veterinaria Brno 76 (2): 291–299. DOI: 10.2754/avb200776020291
  • Palíková M., Krejčí R., Hilscherová K., Babica P., Navrátil S., Kopp R., Bláha L. 2007b. Effect of different cyanobacterial biomasses and their fractions with variable microcystin content on embryonal development of carp (Cyprinus carpio L.). Aquatic Toxicology 81 (3): 312–318. DOI: 10.1016/j.aquatox.2007.01.001
  • Rakotobe L., Berkal M., Huet H., Djediat C., Jeannoda V., Bodo B., Mambu L., Crespeau F., Edery M. 2010. Effects of Madagascar yam extracts, Dioscorea antaly, on embryolarval development of medaka fish, Oryzias latipes. Toxicon 55 (1): 87–91. DOI: 10.1016/j.toxicon.2009.07.002
  • Scholz S., Fischer S., Gündel U., Küster E., Luckenbach T., Voelker D. 2007. The zebrafish embryo model in environmental risk assessment—applications beyond acute toxicity testing. Environmental Science and Pollution Research 15 (5): 394–404. DOI: 10.1007/s11356-008-0018-z
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