Lack of migration and neurological benefits after infusion of umbilical cord blood cells in ischemic brain injury

• Autorzy: Zawadzka M. , Lukasiuk K. , Machaj E. K. , Pojda Z. , Kaminska B.
• Języki publikacji: EN

Abstrakty

EN

The finding of stem/progenitor cells in postnatal bone marrow and umbilical cord blood, opens up a possibility of using stem cells to treat neurologic diseases. There is a controversy, whether intravenously administered human umbilical cord blood cells (HUCBC) migrate to the brain, differentiate and improve recovery after ischemia. In this study, 13 ×106 cells from non-cultured (non-committed) mononuclear HUCBC fraction were intravenously infused 1, 2, 3 or 7 days after a transient middle cerebral artery occlusion (MCAo) in adult rats. We found few human cells only in the ischemic area, localized mostly around blood vessels with few positive cells in the brain parenchyma. Timing of HUCBC delivery after ischemia or injection of Cyclosporin A at the time of delivery, had no effect on the number of human cells detected in the ischemic brain. Infusion of HUCBC did not reduce infarct volume and did not improve neurologic deficits after MCAo, suggesting that HUCBC failed to migrate/survive in the ischemic brain and did not provide significant neurological benefits.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 1
• Opis fizyczny: p.46-51,fig.,ref.

Twórcy

autor

Zawadzka M.

• Laboratory of Transcription Regulation, The Nencki Institute of Experimental Biology, Warsaw, Poland

autor

Lukasiuk K.

• Laboratory of Transcription Regulation, The Nencki Institute of Experimental Biology, Warsaw, Poland

autor

Machaj E. K.

• Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland
• Institute of Hygiene and Epidemiology, Warsaw, Poland

autor

Pojda Z.

• Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland
• Institute of Hygiene and Epidemiology, Warsaw, Poland

autor

Kaminska B.

• Laboratory of Transcription Regulation, The Nencki Institute of Experimental Biology, Warsaw, Poland

Bibliografia

• Borlongan CV, Hadman M, Sanberg CD, Sanberg PR (2004) Central nervous system entry of peripherally injected umbilical cord blood cells is not required for neuroprotec­tion in stroke. Stroke 35: 2385-2389.
• Bużańska L, Machaj EK, Zabłocka B, Pojda Z, Domańska- Janik K (2002) Human cord blood-derived cells attain neuronal and glial features in vitro. J Cell Sci 115: 2131­2138.
• Chen J, Li Y, Wang L, Zhang ZG, Lu D, Lu M, Chopp M (2001a) Therapeutic benefit of intravenous administration of bone marrow stromal cells after cerebral ischemia in rats. Stroke 32: 1005-1011.
• Chen J, Sanberg PR, Li Y, Wang L, Lu M, Willing AE, Sanchez-Ramos J, Chopp M (2001b) Intravenous adminis­tration of human umbilical cord blood reduces behavioral deficits after stroke in rats. Stroke 32: 2682-2688
• Kozłowska H, Jabłonka J, Janowski M, Jura M, Kossut M, Domańska-Janik K (2007) Transplantation of a novel human cord blood-derived neural-like stem cell line in a rat model of cortical infarct. Stem Cells Dev. 16: 481­488.
• Li Y, Chopp M, Chen J, Wang L, Gautam SC, Xu Y, Zhang ZG (2000) Intrastriatal transplantation of bone marrow stromal cells (MSCs) improves functional recovery after stroke in adult mice. J Cereb Blood Flow Metab 20: 1311-1319.
• Lu D, Sanberg PR, Mahmood A, Li Y, Wang L, Sanchez- Ramos J, Chopp M (2002) Intravenous administration of human umbilical cord blood reduces neurological deficit in the rat after traumatic brain injury. Cell Transplant 11: 275-281.
• Makinen S, Kekarainen T, Nystedt J, Liimatainen T, Huhtala T, Narvanen A, Laine J, Jolkkonen J (2006) Human umbil­ical cord blood cells do not improve sensorimotor or cogni­tive outcome following transient middle cerebral artery occlusion in rats. Brain Res 1123: 207-215.
• Massengale M, Wagers AJ, Vogel H, Weissman IL (2005) Hematopoietic cells maintain hematopoietic fates upon entering the brain. J Exp Med 201: 1579-1589.
• McGuckin CP, Forraz N, Allouard Q, Pettengell R (2004) Umbilical cord blood stem cells can expand hematopoietic and neuroglial progenitors in vitro. Exp Cell Res 295: 350-359.
• Newman MB, Willing AE, Manresa JJ, Davis-Sanberg C, Sanberg PR (2005) Stroke-induced migration of human umbilical cord blood cells: time course and cytokines. Stem Cells Dev 14: 576-586.
• Nystedt J, Makinen S, Laine J, Jolkkonen J (2006) Human cord blood CD34+ cells and behavioral recovery following focal cerebral ischemia in rats. Acta Neurobiol. Exp (Wars) 66: 293-300.
• Pera J, Zawadzka M, Kaminska B, Szczudlik A (2004) Influence of chemical and ischemic preconditioning on cytokine expression after focal brain ischemia. J Neurosci Res 78: 132-140.
• Pera J, Zawadzka M, Kaminska B, Szczudlik A (2005) Neurotrophic Factor Expression after Focal Brain Ischemia Preceded by Different Preconditioning Strategies. Cerebrovasc Dis 19: 247-252.
• Sanchez-Ramos J, Song S, Cardozo-Pelaez F, Hazzi C, Stedeford T, Willing A, Freeman TB, Saporta S, Janssen W, Patel N, Cooper DR, Sanberg PR. (2000) Adult bone mar­row stromal cells differentiate into neural cells in vitro. Exp Neurol 164: 247-256.
• Swanson RA, Morton MT, Tsao-Wu G, Savalos RA, Davidson C, Sharp FR (1990) A semiautomated method for measur­ing brain infarct volume. J Cereb Blood Flow Metab 10: 290-293.
• Vendrame M, Cassady J, Newcomb J, Butler T, Pennypacker KR, Zigova T, Sanberg CD, Sanberg PR, Willing AE (2004) Infusion of human umbilical cord blood cells in a rat model of stroke dose-dependently rescues behavioral defi­cits and reduces infarct volume. Stroke 35: 2390-2395.
• Vendrame M, Gemma C, de Mesquita D, Collier L, Bickford PC, Sanberg CD, Sanberg PR, Pennypacker KR, Willing AE (2005) Anti-inflammatory effects of human cord blood cells in a rat model of stroke. Stem Cells Dev 14: 595­604.
• Xiao J, Nan Z, Motooka Y, Low WC (2005) Transplantation of a novel cell line population of umbilical cord blood stem cells ameliorates neurological deficits associated with isch- emic brain injury. Stem cells Dev 14: 722-733.
• Zawadzka M, Kaminska B (2005) A novel mechanism of FK506-mediated neuroprotection: downregulation of cytokine expression in glial cells. Glia 49: 36-51.
• Zigova T, Song S, Willing AE, Hudson JE, Newman MB, Saporta S, Sanchez-Ramos J, Sanberg PR (2002) Human umbilical cord blood cells express neural antigens after transplantation into the developing rat brain. Cell Transplant 11: 265-274.

Uwagi

PL

Rekord w opracowaniu