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2013 | 16 | 4 |
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Immunohistochemical characteristics and distribution of neurons in the intramural ganglia supplying the urinary bladder in the male pig Z. Pidsudko

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EN
This study investigated the distribution and chemical coding of neurons in intramural ganglia of the urinary bladder trigone (UBT-IG) and cervix (UBC-IG) in the male pig using combined retrograde tracing and double-labelling immunohistochemistry. Additionally, immunoblotting was used to confirm the presence of marker enzymes for main populations of autonomic neurons. Retrograde fluorescent tracer Fast Blue (FB) was injected into the wall of both the left and right side of the bladder trigone, cervix and apex during laparotomy performed under thiopental anaesthesia. Twelve μm-thick cryostat sections were processed for double-labelling immunofluorescence with antibodies against tyrosine hydroxylase (TH), dopamine β-hydroxylase (DBH), neuropeptide Y (NPY), somatostatin (SOM), galanin (GAL), vasoactive intestinal polypeptide (VIP), nitric oxide synthase (NOS), calcitonin gene-related peptide (CGRP), substance P (SP) and vesicular acetylcholine transporter (VAChT). UBT-IG and UBC-IG neurons in both parts of the organ formed characteristic clusters (from few to tens of neuronal cells) found under visceral peritoneum or in the outer muscular layer. Immunohistochemistry revealed several subpopulations in UBT-IG and UBC-IG neurons, namely noradrenergic (ca. 76% and 76%), cholinergic (ca. 22% and 20%), non-adrenergic/non-cholinergic nerve cells (ca. 1.5% and 3.8%), NPY- (ca. 66% and 58%), SOM- (ca. 39% and 39 %), VIP- (ca. 5% and 0%) and NOS- immunoreactive (IR) (ca. 1.5% and 3.8%), respectively. Immunoblotting using antibodies to TH and VAChT showed the presence of studied proteins as revealed by the presence of protein bands of the correct molecular weight. This study has revealed a relatively large population of differently coded UBT- and UBC- IG neurons, which constitute an important element of the complex neuroendocrine system involved in the regulation of the male urogenital organs function.
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-
Rocznik
Tom
16
Numer
4
Opis fizyczny
p.629-638,fig.,ref.
Twórcy
autor
  • Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn, Poland
Bibliografia
  • Andersson KE, Arner A (2004) Urinary bladder contraction and relaxation: physiology and pathophysiology. Physiol Rev 84: 935-986.
  • Arrighi S, Bosi G, Cremonesi F, Domeneghini C (2008) Immunohistochemical study of the pre- and postnatal innervation of the dog lower urinary tract: morphological aspects at the basis of the consolidation of the micturition reflex. Vet Res Commun 32: 291-304.
  • Birder L, Drake M, De Groat WC, Fowler C, Mayer E, Morrison J, Paton J, Griffiths D, Mills IW, Thor K (2009) Neural Control. In: Abrams P, Cardozo L, Khoury S, Wein A (eds) Incotinence, 4th edn. Incontinence. Health Publications Ltd, France, pp 167-254.
  • Callahan SM, Creed KE (1986) Non-cholinergic neurotransmission and the effects of peptides on the urinary bladder of guinea-pigs and rabbits. J Physiol 374: 103-115.
  • Crowe R, Burnstock G (1989) A histochemical and immunohistochemical study of the autonomic innervation of the lower urinary tract of the female pig. Is the pig a good model for the human bladder and urethra? J Urol 141: 414-422.
  • Crowe R, Burnstock G, Light JK (1988) Intramural ganglia in the human urethra. J Urol 140: 183-187.
  • Crowe R, Haven AJ, Burnstock G (1986) Intramural neurons of the guinea-pig urinary bladder: histochemical localization of putative neurotransmitters in cultures and newborn animals. J Auton Nerv Syst 15: 319-339.
  • Dalmose AL, Hvistendahl JJ, Olsen LH, Eskild-Jensen A, Djurhuus JC, Swindle MM (2000) Surgically induced urologic models in swine. J Invest Surg 13: 133-145.
  • De Groat WC, Booth AM (1984) Autonomic system to the urinary bladder and sexual organs. In: Dyck PJ, Thomas PK, Lambert EH, Bunge R, Saunders WB (eds) Peripheral Neuropathy, Philadelphia, pp 285-299.
  • De Groat WC, Booth AM (1993) Synaptic transmission in pelvic ganglia. In: Maggi CA (ed) Nervous control of the urogenital system. Harwood Academic Publ., Chur, Switzerland, pp. 291-347.
  • Dixon JS, Gilpin SA, Gilpin CJ, Gosling JA (1983) Intramural ganglia of the human urinary bladder. Br J Urol 55: 195-198.
  • Dixon JS, Jen PY, Gosling JA (1997) A double-label immunohistochemical study of intramural ganglia from the human male urinary bladder neck. J Anat 190: 125-134.
  • Dixon JS, Jen PY, Gosling JA (1998) Immunohistochemical characteristics of human paraganglion cells and sensory corpuscles associated with the urinary bladder. A developmental study in the male fetus, neonate and infant. J Anat 192: 407-415.
  • Dixon JS, Jen PY, Gosling JA (1999) Tyrosine hydroxylase and vesicular acetylcholine transporter are coexpressed in a high proportion of intramural neurons of the human neonatal and child urinary bladder. Neurosci Lett 277: 157-160.
  • Downie JW (1981) The autonomic pharmacology of the urinary bladder and urethra: a neglected area. Trends Pharmacol Sci 2: 163-165.
  • Edyvane KA, Smet PJ, Trussell DC, Jonavicius J, Marshall VR (1994) Patterns of neuronal colocalisation of tyrosine hydroxylase, neuropeptide Y, vasoactive intestinal polypeptide, calcitonin gene-related peptide and substance P in human ureter. J Auton Nerv Syst 48: 241-255.
  • Feher E, Csanyi K, Vajda J (1979) Ultrastructure of the nerve cells and fibres in the urinary bladder wall of the cat. Acta Anat (Basel) 103: 109-118.
  • Feher E, Csanyi K, Vajda J (1980) Intrinsic innervation of the urinary bladder. Acta Anat (Basel) 106: 335-344.
  • Feher E, Vajda J (1981) Sympathetic innervation of the urinary bladder. Acta Morphol Acad Sci Hung 29: 27-35.
  • Fowler CJ, Griffiths D, De Groat WC (2008) The neural control of micturition. Nat Rev Neurosci 9: 453-466.
  • Gabella G (1990) Intramural neurons in the urinary bladder of the guinea-pig. Cell Tissue Res 261: 231-237.
  • Gabella G, Uvelius B (1990) Urinary bladder of rat: fine structure of normal and hypertrophic musculature. Cell Tissue Res 262: 67-79.
  • Gibson SJ, Polak JM, Anand P, Blank MA, Yiangou Y, Su HC, Terenghi G, Katagiri T, Morrison JF, Lumb BM, et al. (1986) A VIP/PHI-containing pathway links urinary bladder and sacral spinal cord. Peptides 7 (Suppl 1): 205-219.
  • Gilpin CJ, Dixon JS, Gilpin SA, Gosling JA (1983) The fine structure of autonomic neurons in the wall of the human urinary bladder. J Anat 137: 705-713.
  • Gosling JA (1986) The distribution of noradrenergic nerves in the human lower urinary tract. Clin Sci 70 (Suppl 14): 3-6.
  • Grozdanovic Z, Baumgarten HG, Bruning G (1992) Histochemistry of NADPH-diaphorase, a marker for neuronal nitric oxide synthase, in the peripheral autonomic nervous system of the mouse. Neuroscience 48: 225-235.
  • Gu J, Blank MA, Huang WM, Islam KN, McGregor GP, Christofides N, Allen JM, Bloom SR, Polak JM (1984) Peptide-containing nerves in human urinary bladder. Urology 24: 353-357.
  • Hoyle CH (1994) Non-adrenergic, non-cholinergic control of the urinary bladder. World J Urol 12: 233-244.
  • James S, Burnstock G (1988) Neuropeptide Y-like immunoreactivity in intramural ganglia of the newborn guinea pig urinary bladder. Regul Pept 23: 237-245.
  • Jen PY, Dixon JS, Gosling JA (1995) Immunohistochemical localization of neuromarkers and neuropeptides in human fetal and neonatal urinary bladder. Br J Urol 75: 230-235.
  • Klarskov P, Gerstenberg T, Hald T (1984) Vasoactive intestinal polypeptide influence on lower urinary tract smooth muscle from human and pig. J Urol 131: 1000-1004.
  • Kondo H, Kuramoto H, Wainer BH, Yanaihara N (1985) Evidence for the coexistence of acetylcholine and enkephalin in the sympathetic preganglionic neurons of rats. Brain Res 335: 309-314.
  • Krane RJ, Olsson CA (1973) Phenoxybenzamine in neurogenic bladder dysfunction. I. A theory of micturition. J Urol 110: 650-652.
  • Lakomy M, Wasowicz K, Kaleczyc J, Chmielewski S (1990) AChE-positive innervation of the ureters, urinary bladder, and urethra in pigs. Z Mikrosk Anat Forsch 104: 316-326.
  • Landis SC, Fredieu JR (1986) Coexistence of calcitonin gene-related peptide and vasoactive intestinal peptide in cholinergic sympathetic innervation of rat sweat glands. Brain Res 377: 177-181.
  • Lincoln J, Burnstock G (1993) Autonomic innervation of the urinary bladder and urethra. In: Maggi CA (ed) Nervous control of the urogenital system. Harwood Academic Publishers, pp 33-68.
  • Lundberg JM, Hokfelt T (1986) Multiple co-existence of peptides and classical transmitters in peripheral autonomic and sensory neurons-functional and pharmacological implications. Prog Brain Res 68: 241-262.
  • Persson K, Andersson KE (1992) Nitric oxide and relaxation of pig lower urinary tract. Br J Pharmacol 106: 416-422.
  • Pidsudko Z. (2000) Distribution and chemical coding of neurons in the porcine inferior mesenteric ganglion projecting to the urinary bladder trigone. 56. ASGBI//AG/NAV Tripartite Meeting St John’s College Cambridge. Ref Type: Conference Proceeding.
  • Pidsudko Z (2004) Distribution and chemical coding of neurons in intramural ganglia of the porcine urinary bladder trigone. Folia Histochem Cytobiol 42: 3-11.
  • Pidsudko Z, Kaleczyc J, Majewski M, Lakomy M, Scheuermann DW, Timmermans JP (2001) Differences in the distribution and chemical coding between neurons in the inferior mesenteric ganglion supplying the colon and rectum in the pig. Cell Tissue Res 303: 147-158.
  • Prieto D, Benedito S, Rodrigo J, Martinez R, Garcia A (1989) Distribution and density of neuropeptide Y-immunoreactive nerve fibres and cells in the horse urinary bladder. J Auton Nerv Syst 27: 173-180.
  • Rand MJ (1992) Nitrergic transmission: nitric oxide as a mediator of non-adrenergic, non-cholinergic neuro-effector transmission. Clin Exp Pharmacol Physiol 19: 147-169.
  • Schulman CC, Duarte-Escalante O, Boyarsky S (1972) The ureterovesical innervation. A new concept based on a histochemical study. Br J Urol 44: 698-712.
  • Sjogren C, Andersson KE, Husted S (1982) Contractile effects of some polypeptides on the isolated urinary bladder of guinea-pig, rabbit, and rat. Acta Pharmacol Toxicol (Copenh) 50: 175-184.
  • Swindle MM, Moody DC, Phillips LD (1992) Swine as a models in biomedical research. Ames: Iowa State Univ Press, Ames.
  • Thornbury KD, Hollywood MA, McHale NG (1992) Mediation by nitric oxide of neurogenic relaxation of the urinary bladder neck muscle in sheep. J Physiol 451: 133-144.
  • Wasowicz K (2003) Effect of total or partial uterus extirpation on uterus-projecting neurons in porcine inferior mesenteric ganglion. A. Changes in expression of transmitter-synthesizing enzymes-tyrosine hydroxylase, dopamine beta-hydroxylase and choline acetyltransferase. Pol J Vet Sci 6: 131-145.
  • Zhou Y, Ling EA (1998) Colocalization of nitric oxide synthase and some neurotransmitters in the intramural ganglia of the guinea pig urinary bladder. J Comp Neurol 394: 496-505.
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Bibliografia
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