Screening, isolation, and molecular characterization of putative fragrance-related transcripts from Vanda Mimi Palmer

• Autorzy: Mohd-Hairul A.R. , Namasivayam P. , Abdullah J. O. , Cheng Lian G. E.
• Języki publikacji: EN

Abstrakty

EN

The aims of this study were to isolate and characterize putative fragrance-related cDNAs from the floral cDNA library of Vanda Mimi Palmer, an orchid hybrid that has won several international awards for its sweet fragrance. A total of 1,000,000 pfu were screened by hybridizing cDNA library plaques with fully open flower cDNA probe of Vanda Mimi Palmer representing all mRNAs expressed during daytime. The clones that gave positive signals were in vivo excised and PCR-amplified inserts were subjected to reverse-Northern analysis by hybridizing with cDNA probes of fully open flower of Vanda Mimi Palmer (fragrant orchid) and its bud or fully open flower of Vanda Tan Chay Yan (non-fragrant orchid) separately. The clones up-regulated in fully open flower stage of Vanda Mimi Palmer compared to its bud stage or fully open flower of Vanda Tan Chay Yan were sequenced. Sequence analyses showed the presence of eight putative fragrance-related cDNAs of which two were putative Vanda Mimi Palmer 4-(cytidine 5'-diphospho)-2-Cmethyl-D-erythritol kinase (VMPCMEK) and Vanda Mimi Palmer cytochrome P450 protein (VMPCyP450). These two transcripts were selected for full-length cDNA isolation and expression analysis by real-time RT-PCR. The VMPCMEK transcript encodes a polypeptide of 400 amino acid residues, while the VMPCyP450 encodes 538 amino acid residues. Relative expression analysis of VMPCMEK and VMPCyP450 transcripts by real-time RT-PCR showed up-regulated expressions in floral tissues compared to vegetative tissues, and both were found to be developmentally regulated.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 5
• Opis fizyczny: p.1651-1660,fig.,ref.

Twórcy

autor

Mohd-Hairul A.R.

• Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

autor

Namasivayam P.

• Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

autor

Abdullah J. O.

• Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

autor

Cheng Lian G. E.

• Department of Chemistry Faculty of Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

Bibliografia

• Boatright J, Negre F, Chen X, Kish CM, Wood B, Peel G, Orlova I, Gang D, Rhodes D, Dudareva N (2004) Understanding in vivo benzenoid metabolism in petunia petal tissue. Plant Physiol 135:1993–2011
• Bremly PM (1997) Isoprenoid metabolism. In: Dey PM, Harborne JB (eds) Plant biochemistry. Academic Press Ltd, London, pp 417–437
• Chan WS, Abdullah JO, Namasivayam P, Mahmood M (2009) Molecular characterization of a new 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) transcript from Vanda Mimi Palmer. Sci Hortic 121:378–382
• Dudareva N, Pichersky E (2000) Biochemical and molecular genetic aspects of floral scents. Plant Physiol 122:627–633
• Dudareva N, Pichersky E (2006) Floral scent metabolic pathways: their regulation and evolution. In: Dudareva N, Pichersky E (eds) Biology of floral scent. CRC Press, Florida, pp 55–78
• Dudareva N, Martin D, Kish CM, Kolosova N, Gorestein N, Faldt J, Miller B, Bohlman J (2003)(E)-ß-ocimene and myrcene synthase genes of floral scent biosynthesis in snapdragon: functional and expression of three terpene synthase genes of a new terpene synthase subfamily. Plant Cell 15:1227–1241
• Dudareva N, Pichersky E, Gershenzon J (2004) Biochemistry of plant volatiles. Plant Physiol 135:1893–1902
• Ehlting J, Hamberger B, Million-Rousseau R, Werck-Reichhart D (2006) Cytochrome P450 in phenolic metabolism. Phytochem Rev 5:239–270
• Farhi M, Lavie O, Masci T, Hendel-Rahmanim K, Weiss D, Abeliovich H, Vainstein A (2010) Identification of rose phenylacetaldehyde synthase by functional complementation in yeast. Plant Mol Biol 72:235–245
• Flores-Sánchez IJ, Ortega-López J, Montes-Horcasitas MC, Ramos-Valdivia AC (2002) Biosynthesis of sterols and triterpenes in cell suspension cultures of Uncaria tomentosa. Plant Cell Physiol 43:1502–1509
• Frowine SA (2005) Fragrant orchids. Timber Press Inc, Oregon
• Hamdan O (2008) Variasi Dunia Orkid: Panduan Penanaman dan Penjagaan. Alaf 21 Sdn. Bhd, Shah Alam
• Hsiao YY, Tsai WC, Huang TH, Wang HC, Wu TS, Leu YL, Chen WH, Chen HH (2006) Comparison of transcripts in Phalaenopsis bellina and Phalaenopsis equestris (Orchidaceae) flowers to deduce monoterpene biosynthesis pathway. BMC Plant Biol 6:14
• Hsiao YY, Jeng MF, Tsai WC, Chuang YC, Li CY, Kuoh CS, Chen WH, Chen HH (2008) A novel homodimeric geranyl diphosphate synthase from the orchid Phalaenopsis bellina lacking a DD (X)2–4D motif. Plant J 55:719–733
• Joyard J, Ferro M, Masselon C, Seigneurin-Bernya D, Salvia D, Garin J, Rolland N (2009) Chloroplast proteomics and the compartmentation of plastidial isoprenoid biosynthetic pathways. Mol Plant 2:1154–1180
• Kaiser R (1993) The scent of orchids: olfactory and chemical investigations. Elsevier Science Publishers BV, Amsterdam
• Kaminaga Y, Schnepp J, Peel G, Kish CM, Gili BN, Weiss D, Orlova I, Lavie O, Rhodes D, Wood K, Porterfield DM, Cooper AJL, Schloss JV, Pichersky E, Veinstein A, Dudareva N (2006) Plant phenylacetaldehyde synthase is a bifunctional homotetradimeric enzyme that catalyzes phenylalanine decarboxylation and oxidation. J Biol Chem 281:23357–23366
• Kim SM, Kim YB, Kuzuyama T, Kim SU (2008) Two copies of 4-(cystidine 5'-diphospho-2-C-methyl-D-erythritol kinase (CMK) gene in Ginkgo biloba: molecular cloning and functional characterization. Planta 228:941–950
• Lee S, Lee B, Jang I, Kim S, Bhak J (2006) Localizome: a server for identifying transmembrane topologies and TM of eukaryotic proteins utilizing domain information. Nucleic Acids Res 34:99–103
• Lichtenthaler HK (1999) The 1-deoxy-D-xylulose-5-phosphate pathway of isoprenoid biosynthesis in plants. Annu Rev Plant Physiol Plant Mol Biol 50:47–66
• Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using realtime quantitative PCR and the 2DDCt method. Methods 25:402–408
• McGarvey DJ, Croteau R (1995) Terpenoid metabolism. Plant Cell 7:1015–1026
• Mohd-Hairul AR, Namasivayam P, Abdullah JO, Lian GEC (2010) Terpenoid, benzenoid and phenylpropanoid compounds in the floral scent of Vanda Mimi Palmer. J Plant Biol 53: 358–366
• Nagegowda DA, Gutensohn M, Wilkerson CG, Dudareva N (2008) Two nearly identical terpene synthases catalyze the formation of nerolidol and linalool in snapdragon flowers. Plant J 55:224–239
• Ogura K, Koyama T (1998) Enzymatic aspects of isoprenoid chain elongation. Chem Rev 98:1263–1276
• Poulter CD, Rilling HC (1981) Prenyl transferase and isomerase. In: Porter JW, Spurgeon SL (eds) Biosynthesis of isoprenoid compounds vol 1. John Willey and Sons Inc, New Jersey, pp 162–209
• Rodrı'guez-Concepcio'n M, Querol J, Lois LM, Imperial S, Boronat A (2003) Bioinformatic and molecular analysis of hydroxymethylbutenyl diphosphate synthase (GCPE) gene expression during carotenoid accumulation in ripening tomato fruit. Planta 217:476–482
• Rohmer M (1999) The discovery of a mevalonate-independent pathway for isoprenoid biosynthesis in bacteria, algae and higher plants. Nat Prod Rep 16:565–574
• Rothwell GW, Holt B (1997) Fossils and phenology in the evolution of Ginkgo biloba. In: Hori T, Ridge RW, Tulecke W, Tredici PD, Trémouillaux-Guiller J, Tobe H (eds) Ginkgo biloba–a global treasure. Springer-Verlag, Tokyo, pp 223–230
• Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York
• Schaller H (2004) New aspects of sterol biosynthesis in growth and development of higher plants. Plant Physiol Biochem 42:465–476
• Seidenfaden G, Wood JJ (1992) The orchids of peninsular Malaysia and Singapore: a revision of R.E. Holttum: orchids of Malaya. Olsen & Olsen, Fredensborg
• Shalit M, Guterman I, Volpin H, Bar E, Tamari T, Menda N, Zach A, Zamir D, Vainstein A, Weiss D, Pichersky E, Lewinsohn E (2003) Volatile ester formation in roses. Identification of an acetyl-coenzyme A. Geraniol/citronellol acetyltransferase in developing rose petals. Plant Physiol 131:1868–1876
• Su V, Shu BD (2003) Cloning and expression of a putative cytochrome P450 gene that influences the colour of Phalaenopsis flowers. Biotechnol Lett 25:1933–1939
• Suzuki M, Muranaka T (2007) Molecular genetics of plant sterol backbone synthesis. Lipids 42:47–54
• Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599
• Verdonk JC, de Vos CHR, Verhoeven HA, Haring MA, van Tunen AJ, Schuurink RC (2003) Regulation of floral scent production in petunia revealed by targeted metabolomics. Phytochem 62:997–1008

Uwagi

PL

Rekord w opracowaniu