Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2012 | 34 | 5 |
Tytuł artykułu

Seasonal low temperature plays an important role in increasing metabolic content of secondary metabolites in Withania somnifera (L.) Dunal and affects the time of harvesting

Treść / Zawartość
Warianty tytułu
Języki publikacji
Withania somnifera (L.) Dunal is a subtropical crop which is widely used in traditional medicine owing to the presence of root bioactives called withanolides. In the present study, phenological variation in withanolide content in roots and leaves was determined at various days after plantation (DAP). Results showed that during the full vegetative state, the amount of marker secondary metabolites viz., withanolide A (Wd-A) and withanone (Wn), increased significantly (p ≤ 0.05) more than early vegetative state (8.16 and 3.2 times, respectively) and maturity stage (54 and 1.33 times, respectively). To qualify the role of temperature per se in enhancing secondary metabolite content, plants during full vegetative stage were exposed to temperature similar to lowest minimum temperature found during the growing period (8°C). During recovery, the metabolic content of secondary metabolites again fell to the level of plants growing at 25°C. This indicated that seasonal temperature played a key role in increasing secondary metabolites rather than the phenological stage of the plant. The physiological importance of this increase in harvesting and its balance with biomass yield has further been discussed.
Słowa kluczowe
Opis fizyczny
  • Biodiversity and Applied Botany Division, Indian Institute of Integrative Medicine, Canal Road, 180001 Jammu, India
  • Biodiversity and Applied Botany Division, Indian Institute of Integrative Medicine, Canal Road, 180001 Jammu, India
  • Biodiversity and Applied Botany Division, Indian Institute of Integrative Medicine, Canal Road, 180001 Jammu, India
  • Biodiversity and Applied Botany Division, Indian Institute of Integrative Medicine, Canal Road, 180001 Jammu, India
  • Abraham A, Kirson I, Glotter E, Lavie D (1968) A chemotaxonomic study of Withania somnifera (L.) Dun. Phytochemistry 7:957–962
  • Abrol E, Vyas D, Sushma K (2012) Metabolic shift from secondary metabolite production to induction of anti-oxidative enzymes during NaCl stress in Swertia chirata Buch-Ham. Acta Physiologiae Plantarum 34(2):541–546
  • Acamovic T, Brooker JD (2005) Biochemistry of plant secondary metabolites and their effect in animals. Proc Nutr Soc 64:403–412
  • Baldi A, Singh D, Dixit VK (2008) Dual elicitation for improved production of withaferin A by cell suspension cultures of Withania somnifera. App Biochem Biotechnol 151:556–564
  • Brown PD, Tokuhisa JG, Reichelt M, Gershenzon J (2003) Variation of glucosinolate accumulation among different organs and developmental stages of Arabidopsis thaliana. Phytochemistry 62:471–481
  • Budhiraja RD, Krishan P, Sudhir S (2000) Biological activity of withanolides. J Sci Indust Res 59:904–911
  • Cirak C, Radušienė J, Ivanauskas L, Janulis V (2007) Variation of bioactive secondary metabolites in Hypericum origanifolium during its phenological cycle. Acta Physiol Plant 29:197–203
  • Gupta GL, Rana AC (2007) Withania somnifera (ashwagandha): a review. Pharmacogn Rev 1:129–136
  • Janská A, Marška P, Zelenková S, Ovesnǎ J (2010) Cold stress and acclimation—what is important for metabolic adjustment? Plant Biol 12:395–405
  • Khajuria RK, Suri KA, Gupta RK, Satti NK, Amina M, Suri OP, Qazi GN (2004) Separation, identification and quantification of selected withanolides in plant extracts of Withania somnifera by HPLC-UV (DAD)-positive ion electroscopy ionization-mass spectrometry. J Sep Sci 27:541–546
  • Kothari SK, Singh CP, Kumar YV, Singh K (2003) Morphology, yield and quality of ashwagandha (Withania somnifera L. Dunal) roots and its cultivation economics as influenced by tillage depth and plant population density. J Hort Sci Biotechnol 78:422–425
  • Kour K, Pandey A, Suri KA, Satti NA, Gupta KK, Bani S (2009) Restoration of stress induced altered T cell function and corresponding cytokines patterns by withanolide A. Int Immunopharmacol 9:1137–1144
  • Kumar A, Kaul BL, Verma HK (2001) Phenological observations on root yield and chemical composition in different morphotypes of Withania somnifera. J Med Aro Plant Sci 23:21–23
  • Kumar A, Kaul MK, Bhan MK, Khanna PK, Suri KA (2007) Morphological and chemical variation in 25 collections of the Indian medicinal plant, Withania somnifera (L.) Dunal (Solanaceae). Genet Resour Crop Evol 54:655–660
  • Kumar A, Kaul MK, Khanna PK, Koul S, Suri KA (2010) Comparative assessment of growth productivity and chemical profiling of wild and cultivated populations of Withania somnifera (L.) Dunal. In: Gupta VK, Verma AK, Kaul S (eds) Utilisation and management of medicinal plants. Daya Publishing House, New Delhi, pp 183–201
  • Kumar A, Mir BA, Sehgal D, Dar TH, Koul S, Kaul MK, Raina SN, Qazi GN (2011) Utility of multidisciplinary approach for genome diagnostics of cultivated and wild germplasm resources of medicinal Withania somnifera, and the status of new species, W. ashwagandha, in the cultivated taxon. Plant Syst Evol 291:141–151
  • Liu Z, Carpenter SB, Bourgeois WJ, Yu Y, Constantin RJ, Falcon MJ, Adams JC (1998) Variations in the secondary metabolite camptothecin in relation to tissue age and season in Camptotheca acuminata. Tree Physiol 18:265–270
  • Malik F, Singh J, Khajuria A, Suri KA, Satti NK, Singh S, Kaul MK, Kumar A, Bhatia A, Qazi GN (2007) A standardized root extract of Withania somnifera and its major constituent withanolide-A elicit humoral and cell mediated immune response by up regulation of Th1-dominant polarization in BALB/c mice. Life Sci 80:1525–1538
  • Mohan R, Hammers HJ, Mohan PB, Zhan XH, Herbstritt CJ, Ruiz A, Zhang L, Conner BP, Rougas J, Pribluda VS (2004) Withaferin A is a potent inhibitor of angiogenesis. Angiogenesis 7:115–122
  • Namdeo AG, Sharma A, Yadav KN, Gawande R, Mahadik KR, Lopez-Gresa MP, Kim HK, Hae Choi Y, Verpoorte R (2011) Metabolic characterization of Withania somnifera from different regions of India using NMR spectroscopy. Planta Med 77(17): 1958–1964
  • Owais M, Sharad KS, Shehbaz A, Saleemuddin M (2005) Antibacterial activity of Withania somnifera (ashwagandha) an indigenous medicinal plant against experimental murine salmonellosis. Phytomedicine 12:229–235
  • Pandey AK, Chawdhry PK (2006) Propogation techniques and harvesting time on productivity and root quality of Withania somnifera. J Trop Med Plants 7:79–81
  • Shohat B, Glitter C, Abraham A, Lavie D (1967) Antitumor activity of withaferin A. Cancer Chemother Rep 51:271–276
  • Snedecor GW, Cochran WG (1989) Statistical methods, 8th edn. Iowa State University Press, Ames
  • Tripathi AK, Sukla YN, Kumar S (1996) Ashwagandha Withania somnifera Dunal (Solanaceae): a status report. J Med Aro Plant Sci 23:21–23
  • Umadevi P, Sharada AC, Soloman FE (1995) In vivo growth inhibitory and radiosensitizing effects of Withaferin A on mouse Ehrlich ascites carcinoma. Cancer Lett 95:189–193
  • van Dam NM, Bhairo-Marhe SK (1992) Induced chemical defense in Cynoglossum officinale. In: Menken SBJ, Misser JH, Harrewign P (eds) Proceedings of the eighth international symposium on insect–plant relationships, Kluwer, Dordhedht, pp 79–82
  • Zhu J, Dong CH, Zhu JK (2007) Interplay between cold-responsive gene regulation, metabolism and RNA processing during plant cold acclimation. Curr Opinion Plant Biol 10(3):290–295
Rekord w opracowaniu
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.