EN
In Poland, typical morphological deformations of winter oilseed rape (Brassica napus), indicating phytoplasma infection, have been sporadically observed since 2000. In 2009 and 2010, in Wielkopolska Region of Poland, research field surveys were conducted to identify phytoplasmas affecting winter oilseed rape. The diseased plants exhibited symptoms including phyllody of flowers, proliferation of axillary buds, reduction of leaf size and siliques malformations. The phytoplasma presence was confirmed by polymerase chain reaction (PCR) with phytoplasmaspecific primers, sequencing and nucleotide sequence analysis. Phytoplasma infecting oilseed winter rape in Poland was identified as a ‘Candidatus Phytoplasma asteris’ (Aster yellow group, 16Sr I-B phytoplasma subgroup). Phytoplasmatic DNA was detected in plants with both types of symptoms.