Porcine liver vascular bed in Biodur E20 corrosion casts

• Autorzy: Eberlova L. , Liska V. , Mirka H. , Gregor T. , Tonar Z. , Palek R. , Skala M. , Bruha J. , Vycital O. , Kalusova K. , Haviar S. , Kralickova M. , Lametschwandtner A.
• Języki publikacji: EN

Abstrakty

EN

Background: Pigs are frequently used as animal models in experimental medicine. To identify processes of vascular development or regression, vascular elements must be recognised and quantified in a three-dimensional (3D) arrangement. Vascular corrosion casts enable the creation of 3D replicas of vascular trees. The aim of our study was to identify suitable casting media and optimise the protocol for porcine liver vascular corrosion casting. Materials and methods: Mercox II® (Ladd Research, Williston, Vermont, USA) and Biodur E20® Plus (Biodur Products, Heidelberg, Germany) were tested in 4 porcine livers. The resins (volume approximately 700 mL) were injected via the portal vein. Corrosion casts were examined by macro-computed tomography, micro-computed tomography and scanning electron microscopy. Results: For hepatectomies, the operating protocol was optimised to avoid gas or blood clot embolisation. We present a protocol for porcine liver vascular bed casting based on corrosion specimens prepared using Biodur E20® epoxy resin. Conclusions: Only Biodur E20® Plus appeared to be suitable for high-volume vascular corrosion casting due to its optimal permeability, sufficient processing time and minimum fragility. Biodur E20® Plus is slightly elastic, radio-opaque and alcohol-resistant. These properties make this acrylic resin suitable for not only vascular research but also teaching purposes. (Folia Morphol 2016; 75, 2: 154–161)

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2016
• Tom: 75
• Numer: 2
• Opis fizyczny: p.154-161,fig.,ref.

Twórcy

autor

Eberlova L.

• Department of Anatomy, Faculty of Medicine in Pilsen, Charles University in Prague, Karlovarska 48, CZ–301 66 Pilsen, Czech Republic
• Biomedical Centre, Faculty of Medicine in Pilsen, Charles University in Prague, Pilsen, Czech Republic

autor

Liska V.

• Biomedical Centre, Faculty of Medicine in Pilsen, Charles University in Prague, Pilsen, Czech Republic
• Department of Surgery, Charles University Prague, University Hospital in Pilsen, Pilsen, Czech Republic

autor

Mirka H.

• Biomedical Centre, Faculty of Medicine in Pilsen, Charles University in Prague, Pilsen, Czech Republic
• Department of Imaging Methods, Faculty of Medicine in Pilsen, Faculty Hospital in Pilsen, Charles University in Prague, Pilsen, Czech Republic

autor

Gregor T.

• New Technologies - Research Centre, University of West Bohemia, Pilsen, Czech Republic

autor

Tonar Z.

• European Centre of Excellence NTIS, University of West Bohemia, Plzen, Czech Republic

autor

Palek R.

• Biomedical Centre, Faculty of Medicine in Pilsen, Charles University in Prague, Pilsen, Czech Republic
• Department of Surgery, Charles University Prague, University Hospital in Pilsen, Pilsen, Czech Republic

autor

Skala M.

• Biomedical Centre, Faculty of Medicine in Pilsen, Charles University in Prague, Pilsen, Czech Republic
• Department of Surgery, Charles University Prague, University Hospital in Pilsen, Pilsen, Czech Republic

autor

Bruha J.

• Biomedical Centre, Faculty of Medicine in Pilsen, Charles University in Prague, Pilsen, Czech Republic
• Department of Surgery, Charles University Prague, University Hospital in Pilsen, Pilsen, Czech Republic

autor

Vycital O.

• Biomedical Centre, Faculty of Medicine in Pilsen, Charles University in Prague, Pilsen, Czech Republic
• Department of Surgery, Charles University Prague, University Hospital in Pilsen, Pilsen, Czech Republic

autor

Kalusova K.

• Department of Anatomy, Faculty of Medicine in Pilsen, Charles University in Prague, Pilsen, Czech Republic

autor

Haviar S.

• Department of Physics and NTIS - European Centre of Excellence, University of West Bohemia, Plzen, Czech Republic

autor

Kralickova M.

• Biomedical Centre, Faculty of Medicine in Pilsen, Charles University in Prague, Pilsen, Czech Republic
• Department of Physics and NTIS - European Centre of Excellence, University of West Bohemia, Plzen, Czech Republic

autor

Lametschwandtner A.

• Department of Cell Biology, University Salzburg, Salzburg, Austria

Bibliografia

• 1. Aharinejad SH, Lametschwandtner A (1992) Microvascular Corrosion Casting in Scanning Electrone Microscopy. Springer, Berlin-Heidelberg-Wien.
• 2. Bereza T, Tomaszewski KA, Lis GJ, Mizia E, Pasternak A, Mazur M, Mituś J (2014) ‘Venous lakes’ — a corrosion cast scanning electron microscopy study of regular and myomatous human uterine blood vessels. Folia Morphol, 73: 164–168. doi: 10.5603/FM.2014.0024.
• 3. Camprodon R, Solsona J, Guerrero JA, Mendoza CG, Segura J, Fabregat JM (1977) Intrahepatic vascular division in the pig: basis for partial hepatectomies. Arch Surg, 112: 38–40.
• 4. De Cecchis L, Hribernik M, Ravnik D, Gadzijev EM (2000) Anatomical variations in the pattern of the right hepatic veins: possibilities for type classification. J Anat, 197 (Part 3): 487–493.
• 5. Debbaut C, Monbaliu D, Casteleyn C, Cornillie P, Van Loo D, Masschaele B, Pirenne J, Simoens P, Van Hoorebeke L, Segers P (2011) From vascular corrosion cast to electrical analog model for the study of human liver hemodynamics and perfusion. IEEE Trans Biomed Eng, 58: 25–35.
• 6. Debbaut C, Segers P, Cornillie P, Casteleyn C, Dierick M, Laleman W, Monbaliu D (2014) Analyzing the human liver vascular architecture by combining vascular corrosion casting and micro-CT scanning: a feasibility study. J Anat, 224: 509–517. doi: 10.1111/joa.12156.
• 7. Debbaut C, Vierendeels J, Casteleyn C, Cornillie P, Van Loo D, Simoens P, Van Hoorebeke L, Monbaliu D, Segers P (2012) Perfusion characteristics of the human hepatic microcirculation based on three-dimensional reconstructions and computational fluid dynamic analysis. J Biomech Eng, 134: 011003. doi: 10.1115/1.4005545.
• 8. Elias H, Popper H (1955) Venous distribution in livers; comparison in man and experimental animals and application to the morphogenesis of cirrhosis. AMA Arch Pathol, 59: 332–340.
• 9. Fondevila C, Hessheimer AJ, Taurá P, Sánchez O, Calatayud D, de Riva N, Muñoz J, Fuster J, Rimola A, García-Valdecasas JC (2010) Portal hyperperfusion: mechanism of injury and stimulus for regeneration in porcine small-for-size transplantation. Liver Transpl, 16: 364–374.
• 10. Gaudio E, Onori P, Pannarale L, Alvaro D (1996) Hepatic microcirculation and peribiliary plexus in experimental biliary cirrhosis: a morphological study. Gastroenterology, 111: 1118–1124.
• 11. Haenssgen K, Makanya AN, Djonov V (2014) Casting materials and their application in research and teaching. Microsc Microanal, 20: 493–513. doi: 10.1017/S1431927613014050.
• 12. Hodžić A, Zuko A, Avdić R, Alić A, Omeragić J, Jažić A (2013) Influence of fasciola hepatica on serum biochemical parameters and vascular and biliary system of sheep liver. Iran J Parasitol, 8: 92–98.
• 13. Kachlik D, Baca V, Stingl J (2010) The spatial arrangement of the human large intestinal wall blood circulation. J Anat, 216: 335–343. doi: 10.1111/j.1469-7580.2009.01199.x.
• 14. Kahn D, Hickman R, Terblanche J, von Sommoggy S (1988) Partial hepatectomy and liver regeneration in pigs--the response to different resection sizes. J Surg Res, 45: 176–180.
• 15. Kessel GK, Kardon RH (1979) Tissue and organs in SEM. WH Freeman and Company, USA.
• 16. Kondo S, Suzuki R, Yamazaki K, Aihara K (1993) Application of corrosion cast method for scanning electron microscopic observation of mouse embryo vasculature. J Electron Microsc (Tokyo), 42: 14–23.
• 17. Konerding MA, Miodonski AJ, Lametschwandtner A (1995) Microvascular corrosion casting in the study of tumor vascularity: a review. Scanning Microsc, 9: 1233–1243.
• 18. Kralickova A, Eberlova L, Kalusova K, Gregor T, Kochova P, Liska V, Kralickova M, Tonar Z (2014) Quantification of liver microcirculation using X-ray microtomography of vascular corrosion casts. Key Engineering Materials, 592–593: 505–508.
• 19. Kubikova T, Witter K, Liska V, Tonar Z (2014) Morphometry and reconstruction of hepatic lobules in pig based on serial histological sections. In: Fuis V ed. University of Technology, Engineering Mechanics, Brno, pp. 340–343.
• 20. Lametschwandtner A, Lametschwandtner U, Weiger T (1990) Scanning electron microscopy of vascular corrosion casts: technique and applications: updated review. Scanning Microsc, 4: 889–940.
• 21. Lametschwandtner A, Miodonski A, Simonsberger P (1980) On the prevention of specimen charging in scanning electron microscopy of vascular corrosion casts by attaching conductive bridges. Mikroskopie, 36: 270–273.
• 22. Le Couteur DG, Warren A, Cogger VC, Smedsrød B, Sørensen KK, De Cabo R, Fraser R, McCuskey RS (2008) Old age and the hepatic sinusoid. Anat Rec (Hoboken), 291: 661–671. doi: 10.1002/ar.20663.
• 23. Lehmann KS, Ritz JP, Valdeig S, Schenk A, Holmer C, Peitgen HO, Buhr HJ, Frericks BB (2008) Portal vein segmentation of a 3D-planning system for liver surgery: in vivo evaluation in a porcine model. Ann Surg Oncol, 15: 1899–1907. doi: 10.1245/s10434-008-9934-x.
• 24. Masset A, Staszyk C, Gasse H (2006) The blood vessel system in the periodontal ligament of the equine cheek teeth.Part I: The spatial arrangement in layers. Ann Anat, 188: 529–533.
• 25. McCuskey RS (2000) Morphological mechanisms for regulating blood flow through hepatic sinusoids. Liver, 20: 3–7.
• 26. Mogicato G, Vautravers G, Meynaud-Collard P, Deviers A, Sautet J (2015) Blood flows in tributaries of the portal vein: anatomical and angiographic studies in normal beagle dogs. Anat Histol Embryol, 44: 460–467. doi: 10.1111/ahe.12161.
• 27. Motta PM, Murakami T, Fujita H (1992) Scanning electon micrscopy of vascular casts: methods and appilcations. Kluwer Academic Publishers, Boston.
• 28. Ohtani O, Murakami T, Jones AL (1982) Microcirculation of the liver, with spacial reference to the peribiliary portal system. In: Motta PM et DiDio LJA ed. Basic and clinical hepatology. Martinus Nijhoff Publishers, The Hague, Boston, London, pp. 85–96.
• 29. Schwen LO, Preusser T (2012) Analysis and algorithmic generation of hepatic vascular systems. Int J Hepatol, 2012: 357687. doi: 10.1155/2012/357687.
• 30. Shirai W, Sato T, Shibuya H, Naito K, Tsukise A (2005) Three-dimensional vasculature of the bovine liver. Anat Histol Embryol, 34: 354–363.
• 31. Tonar Z, Eberlova L, Polivka J, Daum O, Witter K, Kralickova A, Gregor T, Nedorost L, Kochova P, Rohan E, Kalusova K, Palek R, Skala M, Glanc D, Kralickova M, Liska V (2012) Stereological methods for quantitative assessment of hepatic microcirculation. In: Méndez-Villas A. Current Microscopy Contributions to Advences in Science and Technology. Formatex Research Centre, Badajoz, Spain, pp. 737–748.
• 32. Uršič M, Vrecl M, Fazarinc G (2014) Corrosion cast study of the canine hepatic veins. Folia Morphol, 73: 475–481. doi: 10.5603/FM.2014.0071.
• 33. Zhu J, Chen C, Yang B, Guo Y, Ai H, Ren J, Peng Z, Tu Z, Yang X, Meng Q, Friend S, Huang L (2015) A systems genetics study of swine illustrates mechanisms underlying human phenotypic traits. BMC Genomics,16: 88. doi: 10.1186/s12864-015-1240-y

Identyfikatory

DOI

10.5603/FM.a2015.0094