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2007 | 29 | 2 |
Tytuł artykułu

Leaf explant response in in vitro culture of St. John's wort (Hypericum perforatum L.)

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
For centuries Hypericum perforatum has been used in natural medicine. In the last decades, it has also attracted the attention of pharmaceutical industry due to its promising anti-depressant properties. The important factor in pharmaceutical application of plant material is its stable content of active compounds. Such stability requires standardized conditions of growth, e.g. an in vitro culture. Our aim was to establish a medium allowing for an effective regeneration of shoots from the standardized leaf explants in in vitro conditions. Cultures of the leaf explants carried out in darkness, on Murashige and Skoog agar medium, supplemented with auxins (2,4-dichlorophenoxyacetic acid, 2-metoxy-3,6-dichlorobenzoic acid, α-naphtaleneacetic acid, indole-3-acetic acid) and cytokinins (kinetin, N⁶-(benzyl)adenine, thidiazuron) resulted in callus formation. The callus produced roots on media containing indole-3-acetic acid or α-naphtaleneacetic acid alone. On media supplemented with auxins and cytokinins, indirect shoot organogenesis was also observed. The most efficient shoot formation was observed with 2.85 μM of indole-3-acetic acid and 4.44 μM of benzyladenine. Regenerated shoots were rooted on Murashige and Skoog without plant growth regulators medium or on a medium supplemented with indole-3-acetic acid. From a single leaf explant (one fifth of the leaf) after a month of the culture, 35 regenerated shoots were obtained (allowing for the formation of about 180 vegetative shoots per leaf). Successful multiplication of shoots from a standardized explant makes it possible to obtain a great quantity of uniform plant material for biotechnological purposes.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
29
Numer
2
Opis fizyczny
p.151-156,fig.,ref.
Twórcy
autor
  • Botanical Garden-Centre for Diversity Conservation, Polish Academy of Science, Prawdziwka 2, 02-973 Warsaw, Poland
  • Institute of Plant Experimental Biology, Warsaw University, Miecznikowa 1, 02-096 Warsaw, Poland
  • Institute of Plant Experimental Biology, Warsaw University, Miecznikowa 1, 02-096 Warsaw, Poland
Bibliografia
  • Baruah A, Sarma D, Saud J, Singh RS (2001) In vitro regeneration of Hypericum patulum Thunb.—a medicinal plant. Indian J Exp Biol 39(9):947–949
  • Bilia AR, Gallori S, Vincieri FF (2002) St John’s Wort and depression. Efficacy, safety and tolerability—an update. Life Sci 70:3077–3096
  • Briskin DP, Gawienowski MC (2001) Differential effects of light and nitrogen on production of hypericins and leaf glands in Hypericum preforatum. Plant Physiol Biochem 39: 1075–1081
  • Čellárová E, Kimákova K, Brutóvska R (1992) Multiple shoot formation in Hypericum perforatum L. and variability of R₀. Biol Plant 34(Suppl):536
  • Chatterjee SS, Bhattacharya SK, Wonnemann M, Singer A, Muller WE (1998) Hyperforin as a possible antidepressant component of Hypericum extracts. Life Sci 63:499–510
  • DeSmet P, Nomen WA (1996) ST. John’s wort as an antidepressant. Br J Med 313:241–242
  • Gamborg OL, Miller RA, Ojira K (1968) Nutrient requirement of suspension cultures of soybean root cells. Exp. Cell Res 50:151–158
  • Kirakosyan A, Hayashi H, Inoue K, Charchoglyan A, Vardapetyan H (2000) Stimulation of the production of hypericins by mannan in Hypericum perforatum shoot culture. Phytochemistry 53:345–348
  • Kopleman SH, Augsburger LL, NguyenPho A, Zito WS, Muller FX (2001) Selected Physical and Chemical Properties of Commercial Hypericum perforatum Extracts Relevant for Formulated Product Quality and Performance. AAPS PharmSci 3 (4) article 26
  • Mederos MS (1991) In vitro growth and multiplication of Hypericum canariense L. Acta Hortic 289:133–135
  • Moura M (1998) Conservation of Hypericum foliosum aiton, an endemic azorean species, by miocropropagation. In Vitro Cell Dev Biol Plant 34:244–248
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497
  • Murch SJ, Choffe KL, Victor JMR, Slimmon TY, KrishnaRaj S, Saxena PK (2000a) Thidiazuron-induced regeneration from hypocotyl cultures of St. John’s wort (Hypericum perforatum. cv ‘Anthos’). Plant Cell Rep 19:576–581
  • Murch SJ, KrishnaRaj S, Saxena PK (2000b) Phytopharmaceuticals: problems, limitations and solutions. Sci Rev Altern Med 4:33–38
  • Pasqua G, Avato P, Monacelli B, Santamaria AR, Argentieri MP (2003) Metabolites in cell suspension cultures, calli, and in vitro regenerated organs of Hypericum prrforatum cv. Topas. Plant Sci 165:977–982
  • Pretto FR, Santarém ER (2000) Callus formation and plant regeneration from Hypericum perforatum leaves. Plant Cell Tissue Organ Cult 62:107–113
  • Santarém ER, Astarita LV (2003) Multiple shoot formation in Hypericum perforatum L. and hypericin production. Braz J Plant Physiol 15(1):43–47
  • Schempp CM, Krikin V, Simon-Haarhaus G, Kersten A, Kiss J, Termeer CC, Gilb B, Kaufmann T, Borner C, Sleeman JP, Simon JC (2002) Inhibition of tumor cell growth by hyperforin, a novel anticancer drug from St. John’s wort that acts by induction of apoptosis. Oncogene 21:1242–1250
  • Southwell LA, Bourke CA (2001) Seasonal variation in hypericin content of Hypericum perforatum L. (St. John’s wort). Phytochemistry 56:437–441
  • Thiede HM, Walper A (1994) Inhibition of MAO and COMPT by Hypericum extracts and hypericin. J Geriatr Psychiatry Neurol 1:54–56
  • Walker TS, Bais HP, Vivanco JM (2002) Jasmonic acid-induced hypericin production in cell suspension cultures of Hypericum perforatum L. (St. John’s wort). Phytochemistry 60:289–293
  • Yazaki K, Okuda T (1990) Procyaninins in callus and multiple shoots of Hypericum erectum. Planta Med 56:490–491
  • Zobayed SMA, Saxena PK (2003) In vitro-grown roots: a superior explant for prolific shoot regeneration of St. John’s wort (Hypericum perforatum L. cv ‘New Stem’) in temporary immersion bioreactor. Plant Sci 165:463–470
Typ dokumentu
Bibliografia
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Identyfikator YADDA
bwmeta1.element.agro-a8490a33-12a7-4b41-bc71-50dca236daf1
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