Study on the chemical composition and antioxidant activity of extracts from shoot culture and regenerated plants of Scutellaria altissima L.

• Autorzy: Grzegorczyk-Karolak I. , Kuzma L. , Wysokinska K.
• Języki publikacji: EN

Abstrakty

EN

The flavonoid (baicalin, wogonoside, luteolin, luteolin-7-glucoside) and verbascoside contents of Scutellaria altissima in both shoot cultures, and the shoots and roots of micropropagated plants grown in the greenhouse for 12 weeks or in the field for 2 years were determined. The level of secondary metabolites was found to be strongly affected by the age and type of plant organ. A comparative analysis of S. altissima plants propagated in vitro and from seeds revealed no differences in the level of secondary metabolites when plants of the same age were studied. The antioxidant potential of methanolic extracts from shoot cultures, and the shoots and roots of S. altissima plants propagated in vitro, were evaluated using ABTS radical scavenging, FRAP metal reduction power and the lipid peroxidation test, in relation to the content of baicalin, wogonoside, verbascoside, total phenolic and total flavonoid compounds. Extracts from the roots of field-grown regenerated plants at the flowering stage were found to possess the strongest antioxidant activity. Correlation analysis revealed that the antioxidant activity of extracts correlated most closely with their total phenolic content estimated by the Folin-Ciocalteu method.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2015
• Tom: 37
• Numer: 01
• Opis fizyczny: Article: 1736 [9 p.], fig.,ref.

Twórcy

autor

Grzegorczyk-Karolak I.

• Department of Biology and Pharmaceutical Botany, Medical University of Lodz, ul.Muszynskiego 1, 90-151 Lodz, Poland

autor

Kuzma L.

• Department of Biology and Pharmaceutical Botany, Medical University of Lodz, ul.Muszynskiego 1, 90-151 Lodz, Poland

autor

Wysokinska K.

• Department of Biology and Pharmaceutical Botany, Medical University of Lodz, ul.Muszynskiego 1, 90-151 Lodz, Poland

Bibliografia

• Al-Qudah TS, Shibli RA, Alali FQ (2011) In vitro propagation and secondary metabolites production in wild germander (Teucrium polium L.). In Vitro Cell Dev Biol Plant 47:496–505. doi:10.1007/s11627-011-9352-9
• Beshko NP, Gella EV, Litvinenko VI, Kovalev IP, Gordienko VG (1975) Flavonoids of the roots of Scutellaria altissima. Chem Nat Compd 11:533–534. doi:10.1007/BF00566807
• Buxiang S, Fukuhara M (1997) Effects of coadministration of butylated hydroxytoluene, butylated hydroxyanisole and flavonoid on the activation of mutagens and drug-metabolizing enzymes in mice. Toxicology 122:61–72. doi:10.1016/S0300-483X(97)00078-4
• Chang WH, Chem CH, Lu FJ (2002) Different effects of baicalein, baicalin and wogonin on mitochondrial function, glutathione content and cell cycle progression in human hepatoma cell lines. Planta Med 68:128–132. doi:10.1055/s-2002-20246
• Chen Y, Lu N, Ling Y, Gao Y, Wang L, Sun Y, Qi Q, Feng F, Liu W, You Q, Gao Q (2009) Wogonoside inhibits lipopolysaccharideinduced angiogenesis in vitro and in vivo via toll-like receptor 4 signal transduction. Toxicology 259:10–17. doi:10.1016/j.tox.2009.01.010
• Choi CW, Kim SC, Hwang SS, Choi BK, Ahn HJ, Lee MY, Park SH, Kim SK (2002) Antioxidant activity and free radical scavenging capacity between Korean medicinal plants and flavonoids by assay—guided comparison. Plant Sci 163:1161–1168. doi:10. 1016/S0168-9452(02)00332-1
• Cole IB, Cao J, Alan AR, Saxena PK, Murch SJ (2008) Comparisons of Scutellaria baicalensis, Scutellaria lateriflora and Scutellaria racemosa: genome size, antioxidant potential and phytochemistry. Planta Med 74:474–481. doi:10.1055/s-2008-1034358
• Es-Safi NE, Kerhoas L, Einhorn J, Ducrot PH (2005) Application of ESI/MS, CID/MS and tandem MS/MS to the fragmentation study of eriodictyol 7-O-glucosyl-(1?2)-glucoside and luteolin 7-O-glucosyl-(1?2)-glucoside. Int J Mass Spectrom 247:93–100. doi:10.1016/j.ijms.2005.10.002
• Fabre N, Rustan I, de Hoffmann E, Quetin-Leclercq J (2001) Determination of flavone, flavonol, and flavanone aglycones by negative ion liquid chromatography electrospray ion trap mass spectrometry. J Am Soc Mass Spectrom 12:707–715. doi:10. 1016/S1044-0305(01)00226-4
• Gao Z, Huang K, Yang X, Xu H (1999) Free radical scavenging and antioxidant activities of flavonoids extracted from the radix of Scutellaria baicalensis Georgi. Biochim Biophys Acta 1472:643–650. doi:10.1016/S0304-4165(99)00152-X
• Grzegorczyk-Karolak I, Kuźma Ł, Wysokińska H (2013) The use of long-term Scutellaria altissima callus cultures for shoot regeneration, production of bioactive metabolites and micropropagation. J Med Plants Res 7:3003–3313. doi:10.5897/JMPR2013. 5226
• Halliwell B (2008) Are polyphenols antioxidants or pro-oxidants? What do we learn from cell culture and in vivo studies? Arch Biochem Biophys 476:107–112. doi:10.1016/j.abb.2008.01.028
• Henriquez C, Lopez-Alarcon C, Gomez M, Lutz M, Speisky H (2011) Time-dependence of ferric reducing antioxidant power (FRAP) index in Chilean apples and berries. Arch Latinoam Nutr 61:323–332
• Islam MN, Downey F, Ng CKY (2011) Comparative analysis of bioactive phytochemicals from Scutellaria baicalensis, Scutellaria lateriflora, Scutellaria racemosa, Scutellaria tomentosa and Scutellaria wrightii by LC-DAD-MS. Metabolomics 7:446–453. doi:10.1007/s11306-010-0269-9
• Kitamura K, Honda M, Yoshizaki H, Yamamoto S, Nakane H, Fukushima M, Ono K, Tokunaga T (1998) Baicalin, an inhibitor of HIV-1 production in vitro. Antivir Res 37:131–140. doi:10. 1016/S0166-3542(97)00069-7
• Lamaison JL, Carnat A (1990) Content of principal flavonoids of the flowers and leaves of Crataegus monogyna Jacq. and Crataegus laevigata (Poiret) DC. (Rosaceae). Pharm Acta Helv 65:315–320
• Lim BO (2003) Effects of wogonin, wogonoside, and 3,5,7,20,60- pentahydroxyflavone on chemical mediator production in peritoneal exduate cells and immunoglobulin E of rat mesenteric lymph node lymphocytes. J Ethnopharmacol 84:23–29. doi:10.1016/S0378-8741(02)00257-X
• Matkowski A (2006) Plant phenolic metabolites as antioxidants and antimutagenesis inhibitors. In: Blume Y, Smertenko P, Durzan DJ (eds) NATO life science monographs, vol 376. IOS Press, Amsterdam, pp 129–148
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:472–497
• Pan J, Yuan CS, Lin CJ, Jia ZJ, Zheng RL (2003) Pharmacological activities and mechanisms of natural phenylpropanoid glycosides. Pharmazie 58:767–775. doi:10.1002/chin.200405273
• Petrussa E, Braidot E, Zancani M, Peresson C, Bertolini A, Patui S, Vianelli A (2013) Plant flavonoid—biosynthesis, transport and involvement in stress responses. Int J Mol Sci 14:14950–14973. doi:10.3390/ijms140714950
• Pulido R, Bravo L, Saura-Calixto F (2000) Antioxidant activities of dietary phenols are determined by a modified ferric reducing/antioxidant power assay. J Agric Food Chem 48:3396–3402. doi:10.1021/jf9913458
• Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radicalcation decolorizing assay. Free Radic Biol Med 26:1231–1237. doi:10.1016/S0891-5849(98)00315-3
• Rice-Evans CA, Miller NJ, Paganga G (1996) Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med 20:933–956. doi:10.1016/0891-5849(95)02227-9
• Shang XF, He XR, He XY, Li MX, Zhang RX, Fan PC, Zhang QL, Jia ZP (2010) The genus Scutellaria an ethnopharmacological and phytochemical review. J Ethnopharmacol 128:279–313. doi:10.1016/j.jep.2010.01.006
• Si CL, Liu SC, Hu HY, Jiang JZ, Yu GJ, Ren XD, Xu GH (2013) Activity-guided screening of the antioxidants from Paulownia tomentosa var. tomentosa bark. BioResources 8:628–637
• Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16:144–153
• Tundis R, Nadjafi F, Menichini F (2013) Angiotensin-converting enzyme inhibitory activity and antioxidant properties of Nepeta crassifolia Boiss & Buhse and Nepeta binaludensis Jamzad. Phytother Res 27:572–580. doi:10.1002/ptr.4757
• Wiczańska-Barska A, Królicka A, Głód D, Majdan M, Kawiak A, Krauze-Baranowska M (2012) Enhanced accumulation of secondary metabolites in hairy root cultures of Scutellaria lateriflora following elicitation. Biotechnol Lett 34:1757–1763. doi:10. 1007/s10529-012-0963-y
• Zgórka G (2006) Retention behavior of silica-bonded and novel polymeric reversed-phase sorbents in studies on flavones as chemotaxonomic markers of Scutellaria L. genus. J Chromatogr A 1120:230–236. doi:10.1016/j.chroma.2005.11.109

Identyfikatory

DOI

10.1007/s11738-014-1736-0