The effect of repeated endotoxin injection on gonadotropin secretion in ewes

• Autorzy: Herman A.P. , Kopycinska K. , Krawczynska A. , Romanowicz K. , Tomaszewska-Zaremba D.
• Języki publikacji: EN

Abstrakty

EN

Although it is well known that acute inflammation induced by a single injection of the bacterial endotoxin, lipopolysaccharide (LPS), induces a stress response and suppresses luteinizing hormone (LH) secretion, the effects of repeated endotoxin administration on gonadotropin secretion in sheep have not been studied yet. In this work, the influence of inflammation induced by six days of intravenous LPS injection (400 ng· kg–1 per day) on the release of LH and follicle-stimulating (FSH) hormones was evaluated. Anoestrous ewes were bled twice a day 1 h before and 3 h after LPS injection. Endotoxin injection decreased (P < 0.05) the release of LH from the first day of the experiment. Moreover, on day 5, the suppression of LH secretion was sustained and could also be detected in the samples collected before the next LPS injection. In contrast, elevation (P < 0.05) of FSH release was detected starting from the second dose of the endotoxin. The increased FSH concentration persisted until the end of the experiment. Each LPS injection stimulated (P < 0.05) cortisol release, but from days 3 to 6, this elevation was about three times lower than the level determined during the first two days. The obtained results show that ewes exposed to repeated administration of endotoxin lost their ability to restore proper LH release relatively quickly. This disturbance in gonadotropin secretion could, in part, be associated with the stress induced by LPS, as well as with endotoxin-induced inflammatory challenges.

• Wydawca: -
• Czasopismo: Journal of Animal and Feed Sciences
• Rocznik: 2014
• Tom: 23
• Numer: 3
• Opis fizyczny: p.217-221,fig.,ref.

Twórcy

autor

Herman A.P.

• The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jablonna, Poland

autor

Kopycinska K.

• The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jablonna, Poland

autor

Krawczynska A.

• The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jablonna, Poland

autor

Romanowicz K.

• The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jablonna, Poland

autor

Tomaszewska-Zaremba D.

• The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jablonna, Poland

Bibliografia

• Battaglia D.F., Brown M.E., Krasa H.B., Thrun L.A., Viguie´ C., Karsch F.J., 1998. Systemic challenge with endotoxin stimulates corticotropin-releasing hormone and arginine vasopressin secretion into hypophyseal portal blood: coincidence with gonadotropin-releasing hormone suppression. Endocrinology 139, 4175–4181
• Battaglia D.F., Krasa H.B., Padmanabhan V., Viguié C., Karsch F.J., 2000. Endocrine alterations that underlie endotoxin-induced disruption of the follicular phase in ewes. Biol. Reprod. 62, 45–53
• Coleman E.S., Elsasser T.H., Kemppainen R.J., Coleman D.A., Sartin J.L., 1993. Effect of endotoxin on pituitary hormone secretion in sheep. Neuroendocrinology 58, 111–122
• Dadoun F., Guillaume V., Sauze N., Farisse J., Velut J.G., Orsoni J.C., Gaillard R., Oliver C., 1998. Effect of endotoxin on the hypothalamic-pituitary-adrenal axis in sheep. Eur. J. Endocrinol. 138, 193–197
• Daniel J.A., Abrams M.S., de Souza L., Wagner C.G., Whitlock B.K., Sartin J.L., 2003. Endotoxin inhibition of luteinizing hormone in sheep. Domest. Anim. Endocrinol. 25, 13–19
• Debus N., Breen K.M., Barrell G.K., Billings H.J., Brown M., Young E.A., Karsch F.J., 2002. Does cortisol mediate endotoxin-induced inhibition of pulsatile luteinizing hormone and gonadotropinreleasing hormone secretion? Endocrinology 143, 3748–3758
• Dobson H., Ghuman S., Prabhakar S., Smith R., 2003. A conceptual model of the influence of stress on female reproduction. Reproduction 125, 151–163
• Erickson M.A., Banks W.A., 2011. Cytokine and chemokine responses in serum and brain after single and repeated injections of lipopolysaccharide: Multiplex quantification with path analysis. Brain Behav. Immun. 25, 1637–1648
• Herman A.P., Misztal T., Romanowicz K., Tomaszewska-Zaremba D., 2012. Central injection of exogenous IL-1β in the control activities of hypothalamic-pituitary-gonadal axis in anestrous awes. Reprod. Domest. Anim. 47, 44–52
• Herman A.P., Romanowicz K., Tomaszewska-Zaremba D., 2010. Effect of LPS on reproductive system at the level of the pituitary of anestrous ewes. Reprod. Domest. Anim. 45, e351–e359
• Herman A.P., Tomaszewska-Zaremba D., 2010. Effect of endotoxin on the expression of GnRH and GnRHR genes in the hypothalamus and anterior pituitary gland of anestrous ewes. Anim. Reprod. Sci. 120, 105–111
• Karsch F.J., Battaglia D.F., Breen K.M., Debus N., Harris T.G., 2002. Mechanisms for ovarian cycle disruption by immune/ inflammatory stress. Stress 5, 101–112
• Kokot F., Stupnicki R., 1985. Radioimmunoassay and Competitive Radioimmunoassay Methods Used in the Clinic (in Polish). 2nd Edition. PZWL, Warsaw
• L’Hermite M., Niswender G.D., Reichert L.E., Midgley A.R., 1972. Serum follicle stimulating hormone in sheep as measured by radioimmunoassay. Biol. Reprod. 6, 325–332
• Maeda K., Tsukamura H., 2006. The impact of stress on reproduction: are glucocorticoids inhibitory or protective to gonadotropin secretion? Endocrinology 147, 1085–1086
• Melmed S., 2001. The immuno-neuroendocrine interface. J. Clin. Invest. 108, 1563–1566
• Oakley A.E., Breen K.M., Clarke I.J., Karsch F.J., Wagenmaker E.R., Tilbrook A.J., 2009. Cortisol reduces gonadotropin-releasing hormone pulse frequency in follicular phase ewes: influence of ovarian steroids. Endocrinology 150, 341–349
• Peter A.T., Bosu W.T.K., De Decher R.J., 1989. Suppression of preovulatory luteinizing hormone surges in heifers after intrauterine infusions of Escherichia coli endotoxin. Amer. J. Vet. Res. 50, 368–373
• Stupnicki R., Madej A., 1976. Radioimmunoassay of LH in blood plasma of farm animals. Endokrynologie 68, 6–13
• Tilbrook A.J., Turner A.I., Clarke I.J., 2000. Effects of stress on reproduction in non-rodent mammals: the role of glucocorticoids and sex differences. Rev. Reprod. 5, 105–113
• Tomaszewska-Zaremba D., Herman A.P., Misztal T., 2013. Does central IL-1β affect GnRH secretion in the hypothalamus of anoestrous ewes via different regulatory pathways? J. Anim. Feed Sci. 22, 5–12
• Yoo M.J., Nishihara M., Takahashi M., 1997. Involvement of prostaglandins in suppression of gonadotropin-releasing hormone pulse generator activity by tumor necrosis factor-α. J. Reprod. Dev. 43, 181–187

Uwagi

Rekord w opracowaniu