Evaluation of desmin expression by immunohistochemistry in myocardial cells of dogs with dilated cardiomyopath

• Autorzy: Noszczyk-Nowak A. , Nowak M. , Nicpon J. , Madej J. A.
• Języki publikacji: EN

Abstrakty

EN

The experimental material consisted of 20 dogs: 15 Doberman pinschers with diagnosed dilated cardiomyopathy (DCM), and five healthy dogs (three mixed breeds, one Doberman pinscher, one Boxer). DCM was confirmed by electrocardiography and echocardiography before euthanasia. After euthanasia, an autopsy was performed and myocardial samples were collected from the right and left ventricles, intraventricle septum, and right and left atrium. The samples for the histopathological and immunohistochemical analysis were fixed in with 7% formalin in PBS buffer. The presence of desmin was semiquantitatively evaluated under a light microscope. The normal levels of desmin expression in immunohistochemical staining were observed only in the control dogs. The desmin expression was elevated in 15 dogs with severe DCM The immunochemical method allowed for the evaluation of desmin activity in cardiomyocytes, and showed three patterns of its expression (increase expression, decrease expression, heterogeneous expression). The desmin expression was abnormal in the myocardial samples in dogs with DCM. We observed an increase expression of desmin in 10 dogs with moderate and a decrease expression of desmin in five dogs with advanced DCM. The relationship between the desmin expression and the progression of heart failure secondary to DCM requires further studies.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2009
• Tom: 53
• Numer: 3
• Opis fizyczny: p.509-513,fig.,ref.

Twórcy

autor

Noszczyk-Nowak A.

• Department of Internal and Parasitic Diseases with Clinic for Horses, Dog and Cats, University of Environmental and Life Sciences, 50-366 Wroclaw, Poland

autor

Nowak M.

• Department of Pathological Anatomy, Faculty Veterinary Medicine, University of Environmental and Life Sciences, 50-366 Wroclaw, Poland

autor

Nicpon J.

• Department of Internal and Parasitic Diseases with Clinic for Horses, Dog and Cats, University of Environmental and Life Sciences, 50-366 Wroclaw, Poland

autor

Madej J. A.

• Department of Pathological Anatomy, Faculty Veterinary Medicine, University of Environmental and Life Sciences, 50-366 Wroclaw, Poland

Bibliografia

• 1. Abraham S.C., DeNofrio D., Loh E.: Desmin myopathy involving cardiac, skeletal and vascular smooth muscle: report of case with immunoelectron microscopy. Hum Pathol 1998, 29, 876-882.
• 2. Alroy J., Rush J.E., Freeman L., Amarendhra Kumar M.S., Karuri A., Chase K., Sarkar S.: Inherited infantile dilated cardiomyopathy in dogs: genetic, clinical, biochemical, and morphologic findings. Am J Med Genet 2000, 95, 57-66.
• 3. Ariza A., Coll J., Fernandez-Figueras M.T.: Desmin myopathy: multisystem disorder involving skeletal, cardiac, and smooth muscle. Hum Pathol 1995, 26, 1032-1037.
• 4. Disatian S., Ehrhart E.J., Zimmerman S., Orton E.C.: Interstitial cells from dogs with naturally occurring myxomatous mitral valve disease undergo phenotype transformation. J Heart Valve Dis 2008, 17, 402-411.
• 5. Gao J., Li Z., Paulin D.: A novel site, Mt, in the human desmin enhancer is necessary for maximal expression in skeletal muscle. J Biol Chem 1998, 273, 6402-6409.
• 6. Granger B.L., Lazarides E.: Synemin: a new high molecular weight protein associated with desmin and vimentin filaments in muscle. Cell 1980, 22, 727-738.
• 7. Hein S., Kostin S., Heling A., Maeno Y., Schaper J.: The role of the cytoskeleton in heart failure. Cardiovasc Res 2000, 45, 273-278.
• 8. Heinke M.Y., Wheeler C.H., Chang D., Einstein R., Drake-Holland A., Dunn M.J., dos Remedios C.G.: Protein changes observed in pacing-induced heart failure using two-dimensional electrophoresis. Electrophoresis 1998, 19, 2021-2030.
• 9. Lazarides E., Hubbard B.D.: Immunological characterization of the subunit of the 100 filaments from muscle cells. Proc Natl Acad Sci USC 1976, 73, 4344-4348.
• 10. Kostera-Pruszczyk A., Pruszczyk P., Kamińska A., Lee H.S., Goldfarb L.G.: Diversity of cardiomyopathy phenotypes caused by mutations in desmin. Int J Cardiol. 2007 Dec 19 (Epub ahead in print).
• 11. Oxford E.M., Everitt M., Coombs W., Fox P.R., Kraus M., Gelzer A.R., Saffitz J., Taffet S.M., Moïse N.S., Delmar M.: Molecular composition of the intercalated disc in a spontaneous canine animal model of arrhythmogenic right ventricular dysplasia/ cardiomyopathy. Heart Rhythm 2007, 4, 1196-1205.
• 12. Pawlak A., Gil R.J.: Desmin-an important structural protein of cardiac myocyte. Kardiol Pol 2007, 65, 303-309.
• 13. Pawlak A., Gil R.J., Walczak E., Fidziańska A.: Evaluation of desmin activity using immunohistochemical and immunofluorescent staining of miocardial biopsies in patient with chronic heart failure. Comparison of two methods. Kardiol Pol 2007, 65, 229-235.
• 14. Remmele W., Stegner H.E.: Vorschlag zur einheitlichen Definition eines immunoreaktiven Score (IRS) für den immunohistochemichen Ostrogerezeptor-Nachweis (ERICA) im Mammakarzinomgewebe. Pathologie 1987, 8, 138-140.
• 15. Sharov V.G., Kostin S., Todor A., Schaper J., Sabbah H.N.: Expression of cytoskeletal, linkage and extracellular proteins in failing dog myocardium. Heart Fail Rev 2005, 10, 297-303.
• 16. Stabej P., Imholz S., Versteeg S.A., Zijlstra C., Stokhof A.A., Domanjko-Petric A., Leegwater P.A., van Oost B.A.: Characterization of the canine desmin (DES) gene and evaluation as a candidate gene for dilated cardiomyopathy in the Dobermann. Gene 2004, 340, 241-249.
• 17. Thornell L.E., Eriksson A., Johansson B.: Intermediate filament and associated proteins in heart Purkinje fibers: a membrane-myofibril anchored cytoskeletal system. Ann N Y Acad Sci 1985, 455, 213-240.
• 18. Wiersma A.C., Leegwater P.A., van Oost B.A., Ollier W.E., Dukes-McEwan J.: Canine candidate genes for dilated cardiomyopathy: annotation of and polymorphic markers for 14 genes. BMC Vet Res 2007, 19, 28.
• 19. Wiersma A.C., Stabej P., Leegwater P.A., Van Oost B.A., Ollier W.E., Dukes-McEwan J.: Evaluation of 15 candidate genes for dilated cardiomyopathy in the Newfoundland dog. J Hered 2008, 99, 73-80.