Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2010 | 13 | 4 |
Tytuł artykułu

Effect of platelet activating factor (PAF) supplementation in semen extender on viability and ATP content of cryopreserved canine spermatozoa

Treść / Zawartość
Warianty tytułu
Języki publikacji
The aim of this study was to investigate the effect of platelet activating factor (PAF) on the quality characteristics of cryopreserved canine spermatozoa. Cryopreserved semen of 5 mixed-breed dogs was treated with different concentrations of exogenous PAF (1 × 10⁻³M, 1 × 10⁻⁴M, 1 × 10⁻⁵M and 1 × 10-6M) and examined at different time intervals (0, 30, 60 and 120 min). Cryopreserved semen treated without PAF was used as the control. Sperm quality was evaluated for motility (computer-assisted semen analysis, CASA), mitochondrial function (JC-1/PI assay) and plasma membrane integrity (SYBR-14/PI assay and Hoechst 33258). Also, ATP content of spermatozoa was determined using a bioluminescence assay. Treatment of cryopreserved semen with 1 × 10⁻³ M PAF at 120 min of incubation resulted in significantly higher total sperm motility compared with the control. It was observed that PAF-improved total sperm motility was concurrent with enhanced sperm motility patterns after treatment of cryopreserved semen. Treatment of cryopreserved semen with PAF did not improve either sperm mitochondrial function or plasma membrane integrity, as monitored by different fluorescent membrane markers. Furthermore, ATP content of cryopreserved spermatozoa was significantly higher when PAF was used at a concentration of 1 × 10⁻³ M compared with the control and other PAF treatments, regardless of the incubation time. The findings of this study indicated that treatment with 1 × 10⁻³ M PAF at 120 min of incubation rendered better quality of cryopreserved canine semen, which was associated with improved sperm motility parameters and ATP content. It can be suggested that exogenous PAF addition is beneficial as a supplement for canine semen extender used for cryopreservation.
Opis fizyczny
  • Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland
  • Bielański W (1979) Rozród Zwierząt, 3rd ed. PWRiL, Warszawa, pp 437-478.
  • Dziekońska A, Fraser L, Strzeżek J (2009) Effect of different storage temperatures on the metabolic activity of spermatozoa following liquid storage. J Anim Feed Sci 18: 638-649.
  • Ford WC (2006) Glycolysis and sperm motility: does a spoonful of sugar help the flagellum go around? Hum Reprod Update 12: 269-274.
  • Fraser L, Lecewicz M, Strzeżek J (2002) Fluorometric assessments of viability and mitochondrial status of boar spermatozoa following liquid storage. Pol J Vet Sci 5: 85-92.
  • Garner DL, Johnson LA (1995) Viability assessment of mammalian sperm using SYBR-14 and propidium iodide. Biol Reprod 53: 276-284.
  • Garner DL, Thomas CA (1999) Organelle-specific probe JC-1 identifies membrane potential differences in the mitochondrial function of bovine sperm. Mol Reprod Dev 53: 222-229.
  • Hellstrom WJ, Wang R, Sikka SC (1991) Platelet-activating factor stimulates motion parameters of cryopreserved human sperm. Fertil Steril 56: 768-770.
  • Hung PH, Miller MG, Meyers SA, VandeVoort CA (2008) Sperm mitochondrial integrity is not required for hyperactivated motility, zona binding, or acrosome reaction in the Rhesus Macaque. Biol Reprod 79: 367-375.
  • Kamp G, Busselmann G, Jones N, Wiesner B, Lauterwein J (2003) Energy metabolism and intracellular pH in boar spermatozoa. Reproduction 126: 517-525.
  • Kordan W, Lecewicz M, Tobolski J (2009) Effect of platelet-activating factor on motility, plasmalemma integrity, the process of capacitation and acrosome reaction of fresh and cryopreserved boar spermatozoa. Pol J Vet Sci 12: 175-181.
  • Kordan W, Strzeżek J (2002) Effect of platelet-activating factor (PAF) on motility parameters and plasmalemma integrity of boar spermatozoa. Anim Sci Pap Rep 20: 37-45.
  • Kordan W, Strzeżek J (2006) Synthetic platelet activating factor (PAF) as a boar semen extender component. Med Weter 62: 560-562.
  • Kordan W, Strzeżek J, Fraser L (2003) Functions of platelet-activating factor (PAF) in mammalian reproductive processes: a review. Pol J Vet Sci 6: 55-60.
  • Krausz C, Gervasi G, Forti G, Baldi E (1994) Effect of platelet activating factor on motility and acrosome reaction of human spermatozoa. Hum Reprod 9: 471-476.
  • Linde-Forsberg C (1991) Achieving canine pregnancy by using frozen or chilled extended semen. Vet Clin North Am Small Anim Pract 21: 467-485.
  • Linde-Forsberg C, Forsberg M (1989) Fertility in dogs in relation to semen quality and the time and site of insemination with fresh and frozen semen. J Reprod Fertil Suppl 39: 299-310.
  • O’Meara CM, Hanrahan JP, Prathalingam NS, Owen JS, Donovan A, Fair S, Ward F, Wade M, Evans AC, Lonergan P (2008) Relationship between in vitro sperm functional tests and in vivo fertility of rams following cervical artificial insemination of ewes with frozen-thawed semen. Theriogenology 69: 513-522.
  • Odeh AI, Dascanio JJ, Caceci T, Bowen J, Eng LA (2003) Effect of platelet activating factor (PAF) on stallion sperm motility, capacitation and the acrosome reaction. Reproduction 126: 605-613.
  • Parks JE, Hough SR (1990) Effects of platelet activating factor on the motility and acrosome reaction of bovine spermatozoa. Theriogenology 34: 903-912.
  • Peńa FJ, Nxńez-Marttnez I, Morhn JM (2006) Semen technologies in dog breeding: an update. Reprod Domest Anim 41: 21-29.
  • Ricker DD, Minhas BS, Kumar R, Robertson JL, Dodson MG (1989) The effects of platelet-activating factor on the motility of human spermatozoa. Fertil Steril 52: 655-658.
  • Rota A, Strom B, Linde-Forsberg C, Rodriguez-Martinez H (1997) Effects of equex STM paste on viability of frozen-thawed dog spermatozoa during in vitro incubation at 38℃. Theriogenology 47: 1093-1101.
  • Roudebush WE, Diehl JR (2000) Platelet-activating factor content in boar spermatozoa correlates with fertility. Theriogenology 55: 1633-1638.
  • Strzeżek J, Lecewicz M, Dziekońska A, Fraser L (2005) A simple method of extraction of lipoprotein fractions from avian egg yolk – protective effect on cooled boar semen. Theriogenology 63: 496-497.
  • Thomas CA, Garner DL, DeJarnette JM, Marshall CE (1998) Effect of cryopreservation on bovine sperm organelle function and viability as determined by flow cytometry. Biol Reprod 58: 786-793.
  • Tsutsui T, Tezuka T, Mikasa Y, Sugisawa H, Kirihara N, Hori T, Kawakami F (2003) Artificial insemination with canine semen stored at a low temperature. J Vet Med Sci 65: 307-312.
  • Woelders H (1988) A novel technique for differential live-dead staining using fluorescence ąmicroscopy and fluorimetry. Proceedings of 11th International Congress on Animal Reproduction and Artificial Insemination 4: 579.
  • World Health Organization (1999) Computer-aided sperm analysis (CASA). In: WHO Laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 4th ed. Cambridge University Press, Cambridge, pp 90-93.
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.