Genetic variability of Salmo trutta L. species from the catchment areas of the Drawa and Rega rivers evaluated using RAPD and SSR markers

• Autorzy: Skuza L. , Achrem M. , Pilecka-Rapacz M. , Czerniawski R. , Domagala J. , Kirczuk L.

Warianty tytułu

PL

Ocena zmienności genetycznej gatunków Salmo trutta L. z cieków zlewni Drawy i Regi za pomocą markerów RAPD i SSR

• Języki publikacji: EN

Abstrakty

EN

The knowledge of the genetic variability and structure of Salmo trutta population is needed for effective protection of the species and rational management of the resources. A number of marker systems have been introduced to evaluate the genetic variability of trout populations. Among them, the most often used are the RAPD and SSR markers. Both marker systems are classified as type II markers (O’BRIEN 1991, LERCETEAU-KÖHLER and WEISS 2006). In this study, the genetic variability of the Salmo trutta m. fario and Salmo trutta m. trutta populations from the Rega river, and the three watercourses Sitna, Słopica and Bagnica of the Drawa river catchment area, were analysed. One stream, the Chojnówka (located outside the catchments of the above streams), was used as an extra study area. Based on two marker systems, different results were obtained. In the case of RAPD analysis, all loci were polymorphic in all populations. The use of these marker systems permitted the construction of UPGMA similarity trees. The trees revealed a division of the analysed populations into two groups: one group from the Słopica river and the other group from the remaining watercourses. In the second similarity group, two subgroups can be distinguished: one comprising the population of the sea trout from the Rega river and that of the brown trout from the Sitna river (60.7%), and the other consisting of the parr trout populations from the Chojnówka, Bagnica and Sitna (50.3–79.4%). Between the analysed populations, 100% polymorphism was found. The results indicate a high genetic variability of the studied populations. In the case of SSR analysis, 9 microsatellite loci isolated from five trout populations were described. The number of alleles at these loci ranged from 1 to 5 with an average of 2.8 alleles per locus. The expected heterozygosity ranged from 0.07 to 0.66, with an average of 0.35. The results indicate high genetic variation of the populations studied.

PL

W pracy badano zmienność między populacjami Salmo trutta m. fario i Salmo trutta m. trutta pochodzącymi z Regi oraz trzech cieków zlewni Drawy: Sitnej, Słopicy i Bagnicy na podstawie analizy RAPD i SSR. Do analizy dodano jedną populację – Chojnówkę zlokalizowaną poza zlewnią Drawy. Na podstawie dwóch systemów markerowych otrzymano zróżnicowane wyniki. W przypadku analizy RAPD wszystkie loci były polimorficzne we wszystkich populacjach. Drzewo UPGMA przedstawia podział analizowanych populacji na dwie grupy: do pierwszej należy jedynie populacja Słopicy, a do drugiej wszystkie pozostałe. W pracy stwierdzono także występowanie 100% polimorfizmu między analizowanymi populacjami. W przypadku analizy SSR badano 9 loci mikrosatelitarnych w 5 populacjach. Drzewo UPGMA na podstawie tego systemu markerowego przedstawia podział badanych populacji na dwie grupy podobieństwa: jedną złożoną z populacji z Regi i drugą, do której należą pozostałe populacje. Populacja dorosłych oraz troci w stadium parr z Sitnej tworzą odrębną podgrupę podobieństwa. Otrzymane wyniki wskazują na bardzo duże zróżnicowanie genetyczne badanych ryb.

Słowa kluczowe

EN

genetic variability; genetic polymorphism; microsatellite sequence; brown trout; sea trout; Salmo trutta; catchment area; Drawa River; Rega River; random amplified polymorphic DNA; SSR marker

• Wydawca: -
• Czasopismo: Polish Journal of Natural Sciences
• Rocznik: 2014
• Tom: 29
• Numer: 2
• Opis fizyczny: p.161-175,fig.,ref.

Twórcy

autor

Skuza L.

• Department of Cell Biology, University of Szczecin, Waska 13, 70-415 Szczecin, Poland
• Molecular Biology and Biotechnology Center, University of Szczecin, Szczecin, Poland

autor

Achrem M.

• Department of Cell Biology, University of Szczecin, Waska 13, 70-415 Szczecin, Poland
• Molecular Biology and Biotechnology Center, University of Szczecin, Szczecin, Poland

autor

Pilecka-Rapacz M.

• Department of General Zoology, University of Szczecin, Szczecin, Poland
• Molecular Biology and Biotechnology Center, University of Szczecin, Szczecin, Poland

autor

Czerniawski R.

• Department of General Zoology, University of Szczecin, Szczecin, Poland
• Molecular Biology and Biotechnology Center, University of Szczecin, Szczecin, Poland

autor

Domagala J.

• Department of General Zoology, University of Szczecin, Szczecin, Poland
• Molecular Biology and Biotechnology Center, University of Szczecin, Szczecin, Poland

autor

Kirczuk L.

• Department of General Zoology, University of Szczecin, Szczecin, Poland
• Molecular Biology and Biotechnology Center, University of Szczecin, Szczecin, Poland

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