Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2013 | 57 | 1 |
Tytuł artykułu

Serum concentration of free amino acids in dogs suffering from perianal tumours

Warianty tytułu
Języki publikacji
The aim of the study was to determine serum free amino acid concentration in male dogs suffering from benign and malignant perianal tumours. Serum concentrations of cysteic acid, glutamic acid, glutaminę, aspartic acid, glycine, γ-aminobutyric acid, tryptophan, methionine, arginine, taurine, threonine, tyrosine, alanine, valine, isoleucine, and leucine were significantly changed in dogs suffering from benign and/or malignant tumours when compared to the control group (P<0.05). Serum concentration of serine, phenylalanine, lysine and histidine was not influenced in dogs with neoplastic disease (P>0.05). The evaluation of serum free amino acid concentration, has shown that threonine, glutamic acid, alanine, valine, isoleucine, leucine, γ-aminobutyric acid, and glycine have the highest diagnostic and prognostic value in dogs suffering from benign and malignant perianal tumours. Highly diagnostic and prognostic value in relation to benign tumour growth was also found while evaluating branched chain and acidic groups of amino acids. Serine, phenylalanine, lysine, and histidine, as well as alkaline amino acids were proved not to have diagnostic and prognostic value in dogs with the tumours. Thus, the evaluation of free amino acid concentration may serve diagnostic purposes and help in tumour malignancy differentiation. The elaborated experimental model max be used in further studies on neoplastic disease development and monitoring of applied treatment efficiency.
Słowa kluczowe
Opis fizyczny
  • Department and Clinic of Animal Surgery, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland
  • Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland
  • Department of General Chemistry, Medical University in Lublin, 20-093 Lublin, Poland
  • Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences, 20-950 Lublin, Poland
  • 1. Adamu D., Pathomorphological evaluation and classification of idiopathic tumors in dogs. Med Weter 1992, 48, 200-201.
  • 2. Arnesen K., Gamlem H., Glattre E., Moe L., Nordstoga K.: Registration of canine cancer. Tidsskr Nor Laegeroren 1995, 28, 714-717.
  • 3. Berrocal A., Vos J.H., van den Ingh T.S., Molenbeek R.F., van Sluijs F.J.: Canine perineal tumours. Zentralbl Veterinarmed A 1989, 36, 739-749.
  • 4. Brodzki A., Pasternak K., Balicki I., Brodzki P., Szponder T., Tatara M.R.: Concentration of free amino acids in mammary gland tumors in dogs. Med Weter 2004, 60, 937-941.
  • 5. Brodzki A., Tatara M.R., Pasternak K., Różańska D., Szponder T.: Free amino acids in skin neoplastic tissues and serum in dogs. Bull Vet Inst Pulawy 2005, 49, 231- 235.
  • 6. Ching N., Grossi C., Jaham G.: Plasma amino acid and serum unesterified fatty acid deficits and the effect of nutritional support in chemotherapy treatment. Surgen 1984, 95, 730-738.
  • 7. Clarke E.F., Lewis A.M., Waterhouse C.: Peripheral amino acid levels in patient with cancer. Cancer 1978, 42, 2909-2913.
  • 8. Daemen B.J., Elsinga P.H., Mooibroek J., Paans A.M., Wieringa A.R., Konings A.W., Vaalburg W.: PET measurements of hyperthermia-induced suppression of protein synthesis in tumors in relation to effects on tumor growth. J Nucl Med 1991, 32, 1587-1592.
  • 9. Daemen B.J.G., Elsinga P.H., Paans A.M.J., Lemstra W., Konings A.W.T., Vaalburg W.: Suitability of rodent tumor models for experimental PET with L-[1-11C]- tyrosine and 2-[18F]fluoro-2-deoxy-D-glucose. Int J Rad Appl Instrum B 1991, 18, 503-511.
  • 10. Frese K., Durchfeld B., Eskens U.: Classification and biological properties of skin and mammary gland tumors in dogs and cats. Prakt Tierärtz 1989, 70, 69-84.
  • 11. Hayes H.M.Jr., Wilson G.P.: Hormone-dependent neoplasm of the canine perianal gland. Cancer Res 1977, 37, 2068-2071.
  • 12. Herzog R.E.: Cytophotometric determinations of DNA, histone, arginine, lysine, and their concentrations in eu- and heterohromatin of the cell nucleus of displasias, carcinoma in situ, and carcinoma of the human cervix uteri. Arch Gynecol 1982, 31, 91-98.
  • 13. Hirajama C., Suyama K., Horie Y., Tanimoto K., Kato S.: Plasma amino acid patterns in hepatocellular carcinoma. Biochem Med Metab Biol 1987, 38, 127-133.
  • 14. Jager P.L., Vaalburg W., Pruim J., de Vries E.G., Langen K.J., Piers D.A.: Radiolabeled amino acids, basic aspects and clinical applications in oncology. J Nucl Med 2001, 42, 432-445.
  • 15. Kokolis N., Ziegler I.: On the levels of phenylalanine, tyrosine and tetrahydrobioprterin in the blood of tumor- bearing organisms. Cancer Biochem Biophys 1997, 2, 79-85.
  • 16. Krämer S.D., Mu L., Müller A., Keller C., Kuznetsova O.F., Schweinsberg C., Franck D., Müller C., Ross T.L., Schibli R., Ametamey S.M.: 5-(2-18F-Fluoroethoxy,-L- tryptophan as a substrate of system L transport for tumor imaging by PET. J Nucl Med 2012, 53, 434-442.
  • 17. Kubota A., Meguid M.M., Hith D.C.: Amino acid profiles correlate diagnostically with organ site in three kinds of malignant tumors. Cancer 1992, 69, 2343-2348.
  • 18. Martins A.M.C.R.P.F., Vasques-Peyser A., Torres L.N., Matera J.M., Dagli M.L.Z., Guerra J.L.: Retrospective-systematic study and quantitative analysis of cellular proliferation and apoptosis in normal, hyperplastic and neoplastic perianal glands in dogs. Vet Comp Oncol 2008, 6,71-79.
  • 19. Nefyodov L.I., Uglyanica K.N., Smirnov V.Y., Doroshenko Y.M., Fomin K.A., Nowicky J.W., Brzosko W.J.: Amino acids and their derivatives in tumor tissue from patients with breast cancer treated with Ukrain. Part IV. Drugs Exp Clin Res 1996, 22, 159-161.
  • 20. Nishizaki T., Matsumata T., Taketomi A., Yamamoto K., Sugimachi K.: Levels of amino acids in human hepatocellular carcinoma and adjacent liver tissue. Nutr Cancer 1995, 23, 85-90.
  • 21. Norton J.A., Gorschboth C.M., Wesley R.A., Burt M.E., Brennan M.F.: Fasting plasma amino acid levels in cancer patients. Cancer 1985, 56, 1181 -1186.
  • 22. Okada A., Takehara H., Yoshioka K., Nishi M., Miyake H., Kita Y., Komi N.: Increased aspartate and glutamate levels in both gastric and colon cancer tissues. Tokushima J Exp Med 1993, 40, 19-25.
  • 23. Ollenschlager G., Karner J., Karner-Hanusch J., Jansen S., Schindler J., Roth E.: Plasma glutamate - a prognostic marker of cancer and of other immunodeficiency syndromes. Scand J Clin Lab Invest 1989, 49, 773-777.
  • 24. Jager P.L., Vaalburg W., Pruim J., de Vries E.G., Langen K.J., Piers D.A.: Association of rubidium and C- methionine uptake in brain tumors measured by positron emission tomography. J Neurooncol 1996, 27, 163-171.
  • 25. Ross J.T., Scavelli T.D., Matthiesen D.T., Patnaik A.K.: Adenocarcinoma of the apocrine glands of the anal sac in dogs, a review of 32 cases. J Am Anim Hosp Assoc 1991, 27, 349-355.
  • 26. Russell D.M., Shike M., Anderson G.H.: Amino acid metabolism in small cell lung cancer. J Parenter Enteral Nutr 1981, 6, 259-263.
  • 27. Tatara M.R., Brodzki A., Pyz-Łukasik R., Pasternak K., Szpetnar M.: Sex-related differences in skeletal muscle amino acid concentrations in 20 week old turkeys. J Poult Sci 2012, 49, 219-223.
  • 28. Van Eijk H.M.H., Rooyakkers D.N., Van Acker B.A.C., Soeters P.B., Deutz N.E.P.: Automated isolation of high- purity plasma albumin for isotope ratio measurements. J Chromatography B 1999, 731, 199-205.
  • 29. Von Elling E., Bader K.: Free amino acids in normal and carcinomatous ovarian tissue in correlation with serum level - tumor diagnostic possibilities. Zentralbl Gynakol 1987, 109, 1013-1022.
  • 30. Von Elling E., Bader K., Schicke B.: Free serum amino acids in patients with tumors of different sites - tumor- induced imbalances. Zentralbl Gynakol 1987, 109, 1023- 1032.
  • 31. Watanabe A., Higashi T., Sakata T., Nagashima H.: Serum amino acid levels in patients with hepatocellular carcinoma. Cancer 1984, 54, 1875-1882.
  • 32. Welbourne T., Routh R., Yudkoff M., Nissim I.: The glutamine/glutamate couplet and cellular function. News Physiol Sci 2001, 16, 157-160.
  • 33. Withrow S.J.: Perianal tumours. In: Small Animal Clinical Oncology, edited by S.J. Withrow, E.G. MacEwen, W.B. Saunders Co., Philadelphia, USA, 1997, pp. 261-267.
  • 34. Xi P., Jiang Z., Zheng C., Lin Y., Wu G.: Regulation of protein metabolism by glutamine, implications for nutrition and health. Front Biosci 2011, 16. 578-597.
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.