The effect of luteolin on spermatological parameters, apoptosis, oxidative stress rate in freezing rabbit semen

• Autorzy: Akarsu S.A. , Acısu T.C. , Gungor I.H. , Cakır Cihangiroolu A. , Koca R.H. , Turk G. , Sonmez M. , Gur S. , Fırat F. , Esmer Duruel H.E.
• Języki publikacji: EN

Abstrakty

EN

The aim of the present study was to determine the effects of Luteolin (LUT) on semen quality, oxidative stress, apoptosis, acrosomal integrity, mitochondrial membrane potential and dead sperm ratio in rabbits. Ejaculates from six New Zealand rabbits were collected, evaluated and pooled. The pooling was divided into five groups as control (no additive) LUT 25 μM, LUT 50 μM, LUT 100 μM and LUT 200 μM and LUT added. It was then filled into a falcon tube with Tris-based extender at a final concentration of approximately 35 x 106 spermatozoa. Diluated rabbit semen samples were drawn into frozen and thawed. Frozen semen straws were thawed at 37°C in 30 seconds. According to our findings, no statistical difference was found between all doses of luteolin and the control group in the CASA (computer assisted sperm analysis) analysis performed at 4°C. However, total motility, progressive motility and rapid sperm percentage were found to be higher in the frozen and thawed rabbit semen at a dose of LUT 50 μM compared to the other groups (p<0.05). While amplitude of lateral head displacement (ALH) and beat cross-frequency (BCF) values were found at the lowest dose of LUT 200 μM, a statistically significant difference was observed between the other groups. When the flow cytometry results were examined, no statistical difference was found between the rate of dead sperm, acrosomal integrity, mitochondrial membrane potential and apoptosis rate. Morever, the H2O2 percentage was found to be lower in all experimental groups compared to the control group (p<0.001). In conclusion, the addition of LUT in long-term storage of rabbit semen provided a protective effect for spermatozoa with its antioxidative properties against damage caused by cryopreservation.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2023
• Tom: 26
• Numer: 1
• Opis fizyczny: p.91-98,ref.

Twórcy

autor

Akarsu S.A.

• Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Atatürk University, 25240 Yakutiye /Erzurum, Turkey

autor

Acısu T.C.

• Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Fırat University, 23200, Elazığ, Turkey

autor

Gungor I.H.

• Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Fırat University, 23200, Elazığ, Turkey

autor

Cakır Cihangiroolu A.

• Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Fırat University, 23200, Elazığ, Turkey

autor

Koca R.H.

• Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bingöl University, Selahaddin-i Eyyübi Neighbourhood, University Street No: 1, 12000, Bingöl, Turkey

autor

Turk G.

• Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Fırat University, 23200, Elazığ, Turkey

autor

Sonmez M.

• Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Fırat University, 23200, Elazığ, Turkey

autor

Gur S.

• Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Fırat University, 23200, Elazığ, Turkey

autor

Fırat F.

• Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Fırat University, 23200, Elazığ, Turkey

autor

Esmer Duruel H.E.

• Elbistan Vocational School, Kahramanmaraş İstiklal University, Şehit Astsubay Ömer Halisdemir Street No:5, Doğan Neighbourhooh, 46340, Elbistan, Kahramanmaraş, Turkey

Identyfikatory

DOI

10.24425/pjvs.2023.145010