Binocular deprivation reveals early plasticity-dependent region- and layer- specific zif268 expression profiles in cat primary visual cortex (area 17)

• Autorzy: Laskowska-Macios K. , Zapasnik M. , Hu T.T. , Kossut M. , Arckens L. , Burnat K.
• Języki publikacji: EN

Abstrakty

EN

Binocular pattern deprivation (BD) alters visual cortex circuitry development, here for the first time studied via analysis of regionand layer-specific expression of the activity reporter gene zif268. We describe its profile in cat area 17 after BD in comparison to age-matched controls. Three BD conditions were applied; the first four (4BD) or six (6BD) months from eye-opening, and a late onset BD in the 3rd and 4th month of age, preceded by 2 months of normal vision (2N2BD). Cats were exposed to overnight darkness prior to 1-hour light stimulation and then sacrificed; kittens at the end of the BD period, adults after 2 years of visual training upon BD. Radioactive in situ hybridization was applied to analyze zif268 mRNA levels. Juvenile and adult BD cats showed elevated zif268 mRNA levels in peripheral area 17 compared to central area 17, while in controls the signal was similar throughout area 17. 2N2BD kittens had such a BD pattern only in layer IV. All juvenile control and BD kittens, except 4BD, showed a similar inter-laminar zif268 expression profile for central area 17. The least active was layer IV and the most active layer V/VI. Adult control and BD cats displayed such a pattern throughout area 17. Furthermore in BD kittens, layers IV and II/III had a higher level of zif268 mRNA than in control area 17. 4BD resulted in a distinctive elevation of the zif268 mRNA level: in adults in all peripheral area 17 layers as compared to controls; in juveniles in layer IV throughout area 17 and in central layer II/III compared to controls and other BD kittens. We conclude that the quality of visual input during the initial 4 months of life plays a crucial role in establishing the inter-laminar circuitry within primary visual cortex in cat. We suggest that early BD arrests the developmental processes in central and peripheral representations leading to a continued differential zif268 gene expression ratio in central versus peripheral area 17 into adulthood. Project co-financed by the European Union from the European Regional Development Fund within the frame of International PhD Projects Programme (MPD4-504).

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2011
• Tom: 71
• Numer: S
• Opis fizyczny: p.38-39

Twórcy

autor

Laskowska-Macios K.

• Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology PAS, Warsaw, Poland

autor

Zapasnik M.

• Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology PAS, Warsaw, Poland

autor

Hu T.T.

• Neuroplasticity and Neuroproteomics Unit, K.U. Leuven, Leuven, Belgium

autor

Kossut M.

• Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology PAS, Warsaw, Poland

autor

Arckens L.

• Neuroplasticity and Neuroproteomics Unit, K.U. Leuven, Leuven, Belgium

autor

Burnat K.

• Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology PAS, Warsaw, Poland

Uwagi

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