Elucidating the function of CPEB proteins in microglia

• Autorzy: Kaczmarczyk L. , Turimella S. , Vangoor V. , Seifert G. , Dublin P. , Neumann H. , Steinhauser C. , Theis M.
• Języki publikacji: EN

Abstrakty

EN

Cytoplasmic Polyadenylation Element Binding proteins (CPEBs) modulate local protein synthesis in neurons crucial for LTP maintenance, memory, and learning. Our recent results indicate they are involved in the progression of epilepsy in a mouse model of temporal lobe epilepsy (TLE). Neuronal proteins regulated by CPEBs include tissue plasminogen activator (tPA), but the primary source of tPA in brain is microglia. CPEB function in glial cells has hardly been investigated. Since microglial tPA is a crucial neurodegenerative factor in TLE, we explore the attractive hypothesis of microglial CPEB involvement in tPA release and, thereby, neurodegeneration following seizures. By single cell RT-PCR we observed CPEB1-4 mRNAs in mouse microglia. This corroborates RT-PCR and immunoblot results, showing abundant CPEB transcript and protein levels in the BV-2 microglial cell line and ES-cells-derived microglia. We use CX3CR1 EGFP/+ knock-in transgenic mice to isolate microglial cells by FACS. In such primary microglia cultures, we will compare CPEB and tPA expression, polyadenylation state of tPA mRNA, and phosphorylation status of CPEBs before and after microglial activation. Finally, we will look at CPEB-mediated control of tPA expression with a luciferase reporter assay. We hope the abovementioned studies to shed a new light on the function of microglial CPEBs and their potential role in neurodegeneration associated with TLE.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 3
• Opis fizyczny: p.339

Twórcy

autor

Kaczmarczyk L.

• Institute of Cellular Neurosciences, University Bonn LIFE and BRAIN Center, Bonn, Germany

autor

Turimella S.

• Institute of Cellular Neurosciences, University Bonn LIFE and BRAIN Center, Bonn, Germany

autor

Vangoor V.

• Institute of Cellular Neurosciences, University Bonn LIFE and BRAIN Center, Bonn, Germany

autor

Seifert G.

• Institute of Cellular Neurosciences, University Bonn LIFE and BRAIN Center, Bonn, Germany

autor

Dublin P.

• Institute of Cellular Neurosciences, University Bonn LIFE and BRAIN Center, Bonn, Germany

autor

Neumann H.

• Institute of Reconstructive Neurobiology, University Bonn LIFE and BRAIN Center, Bonn, Germany

autor

Steinhauser C.

• Institute of Cellular Neurosciences, University Bonn LIFE and BRAIN Center, Bonn, Germany

autor

Theis M.

• Institute of Cellular Neurosciences, University Bonn LIFE and BRAIN Center, Bonn, Germany