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2016 | 65 | 3 |
Tytuł artykułu

Basidiospore and protoplast regeneration from raised fruiting bodies of pathogenic Ganoderma boninense

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Języki publikacji
Ganoderma boninense, a phytopathogenic white rot fungus had sought minimal genetic characterizations despite huge biotechnological potentials. Thus, efficient collection of fruiting body, basidiospore and protoplast of G. boninense is described. Matured basidiocarp raised under the glasshouse conditions yielded a total of 8.3 × 10⁴ basidiospores/ml using the low speed centrifugation technique. Mycelium aged 3-day-old treated under an incubation period of 3 h in lysing enzyme from Trichoderma harzianum (10 mg/ml) suspended in osmotic stabilizer (0.6 M potassium chloride and 20 mM dipotassium phosphate buffer) yielded the highest number of viable protoplasts (8.9 × 10⁶ single colonies) among all possible combinations tested (regeneration media, age of mycelium, osmotic stabilizer, digestive enzyme and incubation period).
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Opis fizyczny
  • Institute of Tropical Agriculture, Universiti Putra Malaysia, Selangor D.E., Malaysia
  • Institute of Tropical Agriculture, Universiti Putra Malaysia, Selangor D.E., Malaysia
  • Department of Biochemistry and Biomolecular Sciences, Faculty of Biotechnology, Universiti Putra Malaysia, Selangor D.E., Malaysia
  • Malaysian Palm Oil Board (MPOB), Persiaran Institusi, Bandar Baru Bangi, Selangor D.E., Malaysia
  • Institute of Tropical Agriculture, Universiti Putra Malaysia, Selangor D.E., Malaysia
  • Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Selangor D.E., Malaysia
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