Survey of single strand conformation polymorphism of kappa-casein gene in Alpine and Saanen goats in Iran

• Autorzy: Nejhad R.K. , Yousefi S. , Azari M. A.
• Języki publikacji: EN

Abstrakty

EN

To study the genetic polymorphism of κ-casein (CSN3) gene as candidate gene for marker-assisted selection in different domestic livestock species, blood samples were randomly withdrawn from 34 Alpine and 42 Saanen goats. DNA was extracted from blood samples and a 416 bp fragment from exon 4 of the CSN3 gene was amplified by polymerase chain reaction (PCR). PCR products were analysed using single strand conformation polymorphism (SSCP) method. Results indicated that there were two (K1 and K2) conformational patterns with frequencies 0.91 and 0.09 for Alpine and 0.76 and 0.24 for Saanen goats, respectively. A Chi-square test confirmed Hardy-Weinberg equilibrium for the gene in both breeds.

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2013
• Tom: 31
• Numer: 2
• Opis fizyczny: p.173-177,fig.,ref.

Twórcy

autor

Nejhad R.K.

• Department of Animal Sciences, Faculty of Agriculture, Gonbad University, P.O.Box 4971799151, Golestan, Gonbad, Iran

autor

Yousefi S.

• Department of Animal Sciences, Gorgan University of Agricultural Sciences and Natural Resources, P.O.Box 49174, Golestan, Iran

autor

Azari M. A.

• Department of Animal Sciences, Gorgan University of Agricultural Sciences and Natural Resources, P.O.Box 49174, Golestan, Iran

Bibliografia

• Ahani Azari M., Yousefi S., Dehnavi E., 2011 – Evaluation of κ-casein and growth hormone genes polymorphism in native Dalagh sheep. Slovak Journal of Animal Science 44(4), 129-133.
• American Dairy Goat Association., 2012 – PO Box 865, Spindale, NC 28160. Phone: [phone] (http://www.adga.org/).
• Barracosa H., 1996 – Estudo de polimorfismos genéticos eda sua associaçăo com capacidades de pro duçăo leiteira em caprinos de raça Algarvia e ovinos de raça Serra da Estrela. Dissertaçăo de Mestrado, Univ. do Algarve, Faro, Portugal.
• Caravaca F., Carrizosa J., Urrutia B., Baena F., Jordana J., Amills M.,Badaoui B., Sanche z A., Angiolillo A., Serradilla J.M.,2009 – Effect of alphaS1-casein (CSN1S1) and kappa-casein (CSN3) genotypes on milk composition in Murciano-Granadina goats. Journal of Dairy Science 92, 2960-2964.
• Caroli A., Jann O., Budelli E., Bolla P., Jager S., Erhardt G., 2001 – Genetic polymorphism of goat κ-casein (CSN3) in different breeds and characterization at DNA level. Animal Genetics 32, 226-230.
• Ceriotti G., Chessa S., Bolla P., Budelli E., Bianchi L., Duranti E., Caroli A., 2004 – Single nucleotide polymorphisms in the ovine casein genes detected by polymerase chain reaction-single strand conformation polymorphism. Journal of Dairy Science 87, 2606-2613.
• Di Luccia A., Mauriello R., Chianese L., Moio L., Addeo F., 1990 – κ-Casein polymorphism in caprine milk. Scienza e Tecnica Lattiero-Casearia 41, 305-314.
• Feligini M., Cubric Curik V., Parma P., Curik I., Greppi G.F.,Enne G., 2002 – Polymorphism of k-Casein in Italian Goat Breeds: A New ACRS-PCR Designed DNA Test for Discrimination of A and B Alleles. Food Technology and Biotechnology 40 (4), 293-298.
• Giambra I.J., Joger S., Erhardt G., 2010 – Isoelectric focusing reveals additional casein variants in German sheep breeds. Small Ruminant Research 90, 11–17.
• Jann O.C., Prinzenberg F.M., Luikart G., Caroli A., Erhardt G., 2004 – High polymorphism in the Kappa-casein (CSN3) gene from wild and domestic caprine species revealed by DNA sequencing. Journal of Dairy Research 71,188-195.
• Kiplagat S.K., Agaba M., Kosgey I.S., Okeyo M., Indetie D., Hanotte O.,Limo M.K.,2010 – Genetic polymorphism of kappa-casein gene in indigenous Eastern Africa goat populations. International Journal of Genetic and Molecular Biology 2,1-5.
• Kumar A., Rout P.K., Mandal A., Roy R., 2009 – Kappaa-casein gene polymorphism in Indian goats. Indian journal of Biotechnology 8,214-217.
• Mandal A., Kumar D., Rout P.K., Yadav N.K., Roy D., 2008 – Genetic polymorphism of milk protein and its effects on milk composition treaits in Muzaffarnagari sheep.i ndian journal of Animal Science 78 (10),81-109.
• Miller S.A, Dykes D.D., Polesky H.F., 1988 – A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research 16(3), 12-15.
• Patel S.B., Pande A.M.,Rank D.N.,Arya J.S.,Jacob N., 2011 – Kappa casein gene polymorphism in Zalawadi goats. Indian journal of Biotechnology 10, 235-237.
• Prinzenberg E.M., Gutscher K., Chessa S., Caroli A., Erhardt G., 2005 – Caprine kappa-Casein (CSN3) Polymorphism: New Developments in Molecular Knowledge. Journal of Dairy Science 88, 1490-1498.
• Rijnkels M., Kooiman P.M., de Boer H.A., Pieper F.R., 1997 – Organization of the bovine casein gene locus. Mammalin Genome 8, 148-152.
• Staiger E.A., Thonney M.L.,Buchanan J.W.Rogers E.R., Oltenac u P.A.,Mateesc u R.G., 2010 – Effect of prolactin, β-lactoglobulin, and κ-casein genotype on milk yield in East Friesian sheep. Journal of Dairy Science 93, 1736- 1742.
• Sztankóová Z., Mátlová V., Kyseľová J., Jandurová O.M., Říha J., Senese C., 2009 – Polymorphism of casein cluster genes in Czech local goat breeds. Journal of Dairy Science 92, 6197–6201.
• Yahyaoui M.H.,Angiolillo A., Pilla F., Sanche z A., Folch J,M., 2003 –Characterization and genotyping of the caprine kappa casein variants. Journal of Dairy Science 86,2715–2720.
• Yahyaoui M.H.,Coll A., Sanchez A., Folch J.M., 2001 – Genetic polymorphism of the caprine kappa casein gene. Journal of Dairy Research 68, 209-216.
• Yeh F.C., Yang R.C., Timothy B.J., Ye Z., Judy M., 1997 – Pop Gene, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Center. Univ. Alberta.