Burkholderia sp. strain TNAU-1 for biological control of root rot in mung bean (Vigna radiata L.) caused by Macrophomina phaseolina

• Autorzy: Satya V.K. , Vijayasamundeeswari A. , Paranidharan V. , Velazhahan R.

Warianty tytułu

PL

Szczep TNAU-1 bakterii Burkholderia sp. jako czynnik biologicznego zwalczania zgnilizny korzeni fasoli złotej (Vigna radiata L.) powodowanej przez Macrophomina phaseolina

• Języki publikacji: EN

Abstrakty

EN

The potential of Burkholderia sp. strain TNAU-1 for the management of mung bean (Vigna radiata L.) root rot caused by Macrophomina phaseolina was evaluated under greenhouse conditions. Burkholderia sp. strain TNAU-1 inhibited the mycelial growth of M. phaseolina in vitro and produced an inhibition zone of 18.8 mm. Mung bean seeds when treated with the bacterial suspension, showed significant increase in root length, shoot length and seedling vigour. A talc-based powder formulation of Burkholderia sp. strain TNAU-1 was developed and evaluated for its efficacy in the management of mung bean root rot under greenhouse conditions. Seed treatment or soil application of the powder formulation of Burkholderia sp. strain TNAU-1 significantly reduced the incidence of root rot and increased the germination percentage and plant height. Seed treatment with the powder formulation of Burkholderia sp. strain TNAU-1 alone was effective in controlling root rot disease; but the combined seed treatment and soil application of Burkholderia sp. strain TNAU-1, increased the efficacy. Seed treatment and soil application with Burkholderia sp. reduced the root rot incidence from 52.6 per cent (with non-bacterized seeds) to 16.7 per cent. Control of root rot with the application of Burkholderia sp. by seed treatment and soil application was not statistically different from that obtained with seed treatment with carbendazim. The endophytic movement of Burkholderia sp. in the stem, roots and leaves of mung bean was confirmed through PCR using Burkholderia sp. specific primers which resulted in the amplification of a 417 bp product.

PL

Oceniano możliwość wykorzystania szczepu TNAU-1 bakterii Burkholderia sp. jako czynnika biologicznego zwalczania zgnilizny korzeni fasoli złotej (Vigna radiata L.) powodowanej przez grzyb Macrophomina phaseolina w warunkach szklarniowych. Szczep TNAU-1 bakterii Burkholderia sp. hamował in vitro wzrost grzybni M. phaseolina i wytwarzał strefę inhibicji równą 18,8 mm. Siewki roślin fasoli wyrastające z nasion potraktowanych zawiesiną bakterii wykazały istotny wzrost długości korzeni i łodyg, a także zwiększony wigor. Opracowano preparat zawierający szczep TNAU-1 bakterii Burkholderia sp. w formie proszku, na bazie talku, do zwalczania zgnilizny korzeni w warunkach szklarniowych. Preparat zastosowany bezpośrednio na nasiona lub doglebowo istotnie ograniczał nasilenie występowania choroby i wpłynął na procent kiełkujących nasion i wysokość roślin. Samo zaprawianie nasion przy pomocy preparatu w formie proszku było skuteczne przeciwko zgniliźnie korzeni, jednak zaprawianie nasion połączone z doglebowym stosowaniem preparatu zwiększało jego skuteczność. Zaprawianie nasion łącznie z zastosowaniem bakterii Burkholderia sp. spowodowało ograniczenie występowania zgnilizny korzeni z 52,6% (nasiona nie traktowane bakteriami) do 16,7%. Skuteczność zwalczania zgnilizny korzeni przy wykorzystaniu bakterii Burkholderia sp. nie różniła się istotnie od działania preparatu karbendazym, zastosowanego do zaprawiania nasion. Przemieszczanie się endofitycznych bakterii Burkholderia sp. w korzeniach, łodydze i liściach potwierdzono przy pomocy metody PCR, wykorzystując specyficzne startery dla bakterii Burkholderia sp., dające amplifikację produktu wielkości 417 pz.

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2011
• Tom: 51
• Numer: 3
• Opis fizyczny: p.273-278,fig.,ref.

Twórcy

autor

Satya V.K.

• Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University Coimbatore-641 003, Tamil Nadu, India

autor

Vijayasamundeeswari A.

autor

Paranidharan V.

autor

Velazhahan R.

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