Molecular detection of bovine leukemia virus in peripheral blood of Iranian cattle, camel and sheep

• Autorzy: Nekoei S. , Taktaz Hafshejani T. , Doosti A. , Khamesipour F.
• Języki publikacji: EN

Abstrakty

EN

Bovine leukemia virus (BLV) is a deltaretrovirus which infects and induces proliferation of B-lymphocytes in the peripheral blood circulation and in lymphoid organs primarily of cattle, leading to leukemia/lymphoma. This study was carried out to investigate the presence of BLV in cattle, sheep and camels from the Chaharmahal va Bakhtiary and Isfahan provinces in Iran. A total of 874 blood samples collected from cattle, sheep and camels were used in this study to detect BLV using a nested- PCR. The results from this study indicated that 17.2% (n=874) of all blood samples collected were positive for BLV. The percentages of blood samples positive for BLV from cattle, sheep and camels were 22.1 (n=657), 5.3 (n=95) and 0 (n=122) respectively. The results from this study showed that BLV infected cattle and sheep. Camels seemed to be resistant to BLV infection. This study contributes to the nationwide effort to obtain baseline information on the prevalence of BLV, which will assist in planning the control strategy for the disease in Iran.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2015
• Tom: 18
• Numer: 4
• Opis fizyczny: p.703-707,fig.,ref.

Twórcy

autor

Nekoei S.

• Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

autor

Taktaz Hafshejani T.

• Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

autor

Doosti A.

• Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

autor

Khamesipour F.

• Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Bibliografia

• Del Fava C, Bastlio ML, de Donato TM, Ribeiro CP, Okuda LH, de Stefano E, Pituco EM (2010) Occurrence of seropositive sheep (Ovis aries) to Bovine Leukemia Virus in Brazil. Braz J Vet Res Anim 47: 483-487.
• Fenner FJ, White DO (2011) Members of the Sub-family Orthoretrovirinae, Genus Detlaretrovirus. In: 4th ed., NJ MacLachlan and Dubovi JE (eds) Fenner’s Veterinary Virology, Amsterdam: Elsevier Inc., pp 264-266.
• Giangaspero M, Orusa R, Savini G, Gennaro AD, Osawa T, Harasawa R (2013) Serological Survey to Determine the Occurrence of Blue Tongue Virus, Bovine Leukemia Virus and Herpesvirus Infections in the Japanese Small Ruminant Population from Northern Districts. Clin Microbiol 2: 104.
• Gonzalez GC, Johnston JB, Nickel DD, Jacobs RM, Olson M, Power C (2001) Very low prevalence of bovine immunodeficiency virus infection in western Canadian cattle. Can J Vet Res 65: 73-76.
• Haghparast A, Tabatabaiezadeh E, Mohammadi G, Kord N (2012) Prevalence of Bovine Leukemia Virus (BLV) Antibodies in Bulk Tank Milk of Dairy Cattle Herds of Mashhad Area, North-East of Iran. JAVA: 11: 276-280.
• Heenemann K, Lapp S, Teifke JP, Fichtner D, Mettenleiter TC, Vahlenkamp TW (2012) Development of a Bovine leukemia virus polymerase gene-based real-time polymerase chain reaction and comparison with an envelope gene-based assay. J Vet Diagn Invest 24: 649 -655.
• Joezy-Shekalgorabi S, Shadparvar A (2009) Overview of Holstein sperm importation in Iran. http://www.eaap.org/Previous–Annual–Meetings/2010Crete/Papers/38–Joezy.pdf. Accessed on 30/07/2014.
• Kazemimanesh M, Madadgar O, Mahzoonieh MR, Zahraei-Salehi T, Steinbach F (2012) A Serological Study on Bovine Leukemia Virus Infection in Ten Provinces of Iran between 2010 and 2012. Iranian J Virol 6: 1-7.
• Khamesipour F, Doosti A, Shahraki AK, GoodarziM (2013) Molecular detection of Bovine Leukemia Virus (BLV) in the frozen semen samples of bulls used for artificial insemination in Iran. ROAVS 3: 412-416.
• Mohammadabadi MR, Soflaei M, Mostafavi H, Honarmand M (2011) Using PCR for early diagnosis of bovine leukemia virus infection in some native cattle. Genet Mol Res 10: 2658-2663.
• Mohammadi V, Atyabi N, Nikbakht Brujen Gh, Lotfollahzadeh S, Mostafavi E (2011) Seroprevalence of Bovine Leukemia Virus in Some Dairy Farms in Iran. Glob Vet 7: 305-309.
• Momtaz H (2010) Cloning and phylogenetic analysis of bovine leukemia virus gag gene in Iranian isolate. Afr J Microbiol Res 4: 218-221.
• Momtaz H, Hemmatzadeh F, Keyvanfar H (2008) Expression of bovine leukemia virus p24 protein in bacterial cell. Pak J Biol Sci 11: 2433-2437.
• Moratorio G, Fischer S, Bianchi S, Tome L, Rama G, Obal G, Carrión F, Pritsch O, Cristina J (2013) A detailed molecular analysis of complete bovine leukemia virus genomes isolated from B-cell lymphosarcomas. Vet Res 44: 19.
• Morovati H, Shirvani E, Noaman V, Lotfi M, Kamalzadeh M, Hatami A, Bahreyari M, Shahramyar Z, Morovati MH, Azimi M, Sakhaei D (2012) Seroprevalence of bovine leukemia virus (BLV) infection in dairy cattle in Isfahan Province, Iran. Trop Anim Health Prod 44: 1127-1129.
• Mousavi S, Haghparast A, Mohammadi G, Tabatabaeizadeh SE (2014) Prevalence of bovine leukemia virus (BLV) infection in the northeast of Iran. Vet Res Forum 5: 135-139.
• Office International Des Epizooties (OIE) (2012) Enzootic bovine leukosis. In OIE Terrestrial Manual, pp 1-11.
• Ott SL, Johnson R, Wells SJ (2003) Association between bovine-leukosis virus seroprevalence and herd-level productivity on US dairy farms. Prev Vet Med 61: 249-262.
• Otto M, Radostits C, Kenneth W, Constable P (2007) Veterinary Medicine: A textbook of the diseases of cattle, horses, sheep, pigs and goats, 10th ed., Edinburgh, London: ELSEVER., pp 1209-1221.
• Rola-Łuszczak M, Finnegan C, Olech M, Choudhury B, Kuźmak J (2013) Development of an improved real time PCR for the detection of bovine leukaemia provirus nucleic acid and its use in the clarification of inconclusive serological test results. J Virol Methods 189: 258-264.
• Sadeghi-Sefidmazgi A, Moradi-Shahrbabak M, Nejati-Javaremi A, Miraei-Ashtiani SR, Amer PR (2012) Breeding objectives for Holstein dairy cattle in Iran. J Dairy Sci 95: 3406-3418.
• Sambrook J, Russell DW (2001) Molecular cloning. Laboratory Manual. New York: Cold Spring Harbor Laboratory Press, Cold Spring Harbor, pp 58-152.
• Wang H, Norris KM, Mansky LM (2002) Analysis of bovine leukemia virus gag membrane targeting and late domain function. J Virol 76: 8485- 8493.

Identyfikatory

DOI

10.1515/pjvs-2015-0091