An improved method for RNA isolation from plants using commercial extraction kits

• Autorzy: Kalinowska E. , Chodorska M. , Paduch-Cichal E. , Mroczkowska K.
• Języki publikacji: EN

Abstrakty

EN

Isolation of RNA from plants rich in secondary metabolites using commercial kits often results in contaminated preparations which are not suitable for downstream applications. Although many specific protocols appropriate for plants with a high content of phenolics, anthocyanins and polysaccharides have been developed, these are often expensive, time consuming and not applicable to different types of tissues. This study presents a simple and efficient modification of RNA extraction from different types of tissues using two commercial reagent kits. By simple improvement, we routinely obtained high-quality RNA of the following plants: the blackcurrant bush, black chokeberry bush, pear tree, apricot tree, apple tree, hardy kiwi, tangerine tree, highbush blueberry and cranberry plant.

Słowa kluczowe

EN

isolation method; RNA isolation; plant; commercial extraction kit; secondary metabolite; total RNA; phenolics; anthocyanin; polysaccharide; high quality RNA; black currant bush; black chokeberry bush; pear tree; apricot tree; apple tree; tangerine tree; cranberry; Northern highbush blueberry; hardy kiwifruit

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2012
• Tom: 59
• Numer: 3
• Opis fizyczny: p.391-393,fig.,ref.

Twórcy

autor

Kalinowska E.

• Department of Plant Pathology, Faculty of Horticulture and Landscape Architecture, Warsaw University of Life Sciences - SGGW, Warsaw, Poland

autor

Chodorska M.

autor

Paduch-Cichal E.

autor

Mroczkowska K.

Bibliografia

• Camacho-Villasana YM, Ochoa-Alejo N, Walling L, Bray EA (2002) An improved method for isolating RNA from dehydrated and nondehydrated Chili pepper (Capsicum annuum L.) plant tissues. Plant Mol Biol Rep 20: 407-414.
• Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol chloroform extraction. Anal Biochem 162: 156-159. 
• Ding LW, Sun QY, Wang ZY, Sun YB, Xu ZF (2008) Using silica particles to isolate total RNA from plant tissues recalcitrant to extraction in guanidine thiocyanate. Anal Biochem 374: 426-428. 
• Ghawana S, Paul A, Kumar H, Kumar A, Singh H, Bhardwaj PK (2011) An RNA isolation system for plant tissues rich in secondary metabolites. BMC Res Notes 4: 85. 
• Liao Z, Chen M, Guo L, Gong Y, Tang F, Sun X, Tang K (2004) Rapid isolation of high-quality total RNA from Taxus and Ginkgo. Prep Biochem Biotechnol 34: 209-214. 
• Loomis MD (1974) Overcoming problems of phenolics and quinones in the isolation of plant enzymes and organelles. Methods Enzymol 31: 528-544. 
• MacKenzie DJ, McLean MA, Mukerji S, Green M (1997) Improved RNA extraction from woody plants for the detection of viral pathogens by reverse transcription-polymerase chain reaction. Plant Dis 81: 222-226.
• Sanchez-Navarro JA, Aparicio F, Herranz MC, Minafra A, Myrta A, Pallas V (2005) Simultaneous detection and identification of eight stone fruit viruses by one-step RT-PCR. Eur J Plant Pathol 111: 77-84.
• Tong Z, Qu S, Zhang J, Wang F, Tao J, Gao Z, Zhang Z (2012) A Modified Protocol for RNA extraction from different peach tissues suitable for gene isolation and Real-Time PCR analysis. Mol Biotechnol 50: 229-236. 
• Verwoerd TC, Dekker BM, Hoekema A (1989) A small-scale procedure for the rapid isolation of plant RNAs. Nucleic Acids Res 17: 2362