Impact of light-emitting diodes (LEDs) on the growth and morphogenesis of encapsulated shoot buds of Curculigo orchioides Gaertn., an endangered medicinal herb

• Autorzy: Gupta S.D. , Kumar A. , Agarwal A.
• Języki publikacji: EN

Abstrakty

EN

The present study describes the impact of light-emitting diodes (LEDs) on synseed germination and subsequent synseed-derived seedling growth of Curculigo orchioides, an endangered medicinal plant under in vitro conditions. Shoot buds regenerated on Murashige and Skoog’s medium (MS) containing 4 mg/l 6-benzylaminopurine (BAP), and 3% sucrose were utilized for encapsulation. Encapsulation was achieved by suspending the excised shoot buds into sodium–alginate gel matrix and dropping them into 100 mM CaCl₂ solution. Synseeds thus produced were incubated under different light conditions. Among the various light treatments, irradiation of synseeds with blue LEDs (BL) strongly influences the growth of the synseed-derived seedlings. A significant increase in length, fresh and dry biomass of the synseed-derived seedlings was observed under BL as compared to light treatments of conventional fluorescence lamps (FL) and a combination of blue and red LEDs with equal proportion (BRL, 1:1). The trifoliate leaf area and weighted density of leaves were measured from the digital images of synseed-derived seedlings using MATLAB® 2013a Image Processing Toolbox. Image analysis of the synseed-derived seedlings also revealed the improved growth in terms of increase in trifoliate leaf area and weighted density under BL. Exposure to red LEDs (RL) resulted in poor synseed germination and severely retarded the growth of the synseed-derived seedlings. The leaves of synseed-derived seedlings grown under BL had higher chlorophyll a, carotenoid, total phenols, and flavonoid contents than that of FL and BRL treatments. The synseed-derived seedlings developed under BL also displayed enhanced levels of antioxidant activity. Ex vitro performance of the synseed-derived seedlings raised under BL was found to be better with improved plant canopy as expressed in terms of increase in trifoliate leaf area and weighted density than other light treatments. The present study for the first time demonstrates the potential of BL irradiation on encapsulation technology with the ability of direct conversion of synseeds to synseed-converted seedlings leading to the vigorous growth of the plantlets which may make the encapsulation strategy ideal for supply of healthy plant propagules for transfer to field conditions and germplasm conservation.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2019
• Tom: 41
• Numer: 04
• Opis fizyczny: Article 50 [12p.], fig.,ref.

Twórcy

autor

Gupta S.D.

• Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, 721302 Kharagpur, India

autor

Kumar A.

• Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, 721302 Kharagpur, India

autor

Agarwal A.

• Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, 721302 Kharagpur, India

Bibliografia

• Agarwal A, Dutta Gupta S (2016) Impact of light-emitting diodes (LEDs) and its potential on plant growth and development in controlled-environment plant production system. Curr Biotechnol 5:28–43
• Agarwal A, Dutta Gupta S, Barman M, Mitra AP (2018) Photosynthetic apparatus plays a central role in photosensitive physiological acclimations affecting spinach (Spinacea oleracea L.) growth in response to blue and red photon flux ratios. Environ Exp Bot 156:170–182
• Ahmed MR, Anis M, Al-Etta HA (2015) Encapsulation technology for short-term storage and germplasm exchange of Vitex trifolia L. Rend Fis Acc Lincei 26:133
• Alvarenga ICA, Pacheco FV, Silva ST, Kelly S, Bertolucci V, Pinto JEBP (2015) In vitro culture of Achillea millefolium L.: quality and intensity of light on growth and production of volatiles. Plant Cell Tissue Organ Cult 122:299–308
• Bafna AR, Mishra SH (2006) Immunostimulatory effect of methanol extract of Curculigo orchioides on immunosuppressed mice. J Ethnopharmacol 104:1–4
• Batista DS, de Castro KM, da Silva AR, Teixeira ML, Sales TA, Soares LI, Cardoso MDG, Santos MDO, Viccini LF, Otoni WC (2016) Light quality affects in vitro growth and essential oil profile in Lippia alba (Verbenaceae). In Vitro Cell Dev Biol Plant 52:276–282
• Batista DS, Felipe SHS, Silva TD, de Castro KM, Mamedes-Rodrigues TC, Miranda NA, Rios-Rios AM, Faria DV, Fortini EA, Chagas A, Torres-Silva G, Xavier A, Arencibia AD, Otoni WC (2018) Light quality in plant tissue culture: does it matter? In Vitro Cell Dev Biol Plant 54:195–215
• Bhattacharyya P, Vijay Kumar V, Johannes Van Staden J (2018) In vitro encapsulation based short term storage and assessment of genetic homogeneity in regenerated Ansellia africana (Leopard orchid) using gene targeted molecular markers. Plant Cell Tissue Organ Cult 133:299–310
• Budiarto K (2010) Spectral quality affects morphogenesis on Anthurium plantlet during in vitro culture. Agrivita 32:234–240
• Bula RJ, Morrow RC, Tibbitts TW, Barta DJ, Ignatius RW, Martin TS (1991) Light-emitting diodes as a radiation source for plants. HortScience 26:203–205
• Chandel KPS, Shukla G, Sharma N (1996) Biodiversity in medicinal and aromatic plants in India. ICAR Publication, India
• Chen M, Chory J, Fankhauser C (2004) Light signal transduction in higher plants. Annu Rev Genet 38:87–117
• Choi CW, Kim SC, Hwang SS, Choi BK, Ahn HJ, Lee MY, Park SH, Kim SK (2002) Antioxidant activity and free radical scavenging capacity between Korean medicinal plants and flavonoids by assay-guided comparison. Plant Sci 163:1161–1168
• Devlin PF, Christie JM, Terry MJ (2007) Many hands make light work. J Exp Bot 58:3071–3077
• Dewir YH, Chakrabarty D, Hahn EJ, Paek KY (2006) The effects of paclobutrazol, light emitting diodes (LEDs) and sucrose on flowering of Euphorbia millii plantlets in vitro. Eur J Hortic Sci 71:240–244
• Dinis TC, Madeira VM, Almeida LM (1994) Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 315:161–169
• Dutta Gupta S, Agarwal A (2017a) Artificial lighting system for plant growth and development: chronological advancement, working principles, and comparative assessment. In: Dutta Gupta S (ed) Light emitting diodes for agriculture. Springer, Singapore, pp 1–25
• Dutta Gupta S, Agarwal A (2017b) Influence of LED lighting on in vitro plant regeneration and associated cellular redox balance. In: Dutta Gupta S (ed) Light emitting diodes for agriculture. Springer, Singapore, pp 273–303
• Dutta Gupta S, Jatothu B (2013) Fundamentals and applications of light-emitting diodes (LEDs) in in vitro plant growth and morphogenesis. Plant Biotechnol Rep 7:211–220
• Dutta Gupta S, Karmakar A (2017) Machine vision based evaluation of impact of light emitting diodes (LEDs) on shoot regeneration and the effect of spectral quality on phenolic content and antioxidant capacity in Swertia chirata. J Photochem Photobiol B: Biol 174:162–172
• Dutta Gupta S, Sahoo TK (2015) Light emitting diode (LED)-induced alteration of oxidative events during in vitro shoot organogenesis of Curculigo orchioides Gaertn. Acta Physiol Plant 37:233
• Fazal H, Abbasi BH, Ahmad N, Ali SS, Akbar F, Kanwal F (2016) Correlation of different spectral lights with biomass accumulation and production of antioxidant secondary metabolites in callus cultures of medicinally important Prunella vulgaris L. J Photochem Photobiol B: Biol 159:1–7
• Ferreira LT, de Araújo Silva MM, Ulisses C, Camara TR, Willadino L (2017) Using LED lighting in somatic embryogenesis and micropropagation of an elite sugarcane variety and its effect on redox metabolism during acclimatization. Plant Cell Tissue Organ Cult 128:211–221
• Ghimire BK, Lee JG, Yoo JH, Kim JK, Yu CY (2017) The influence of light-emitting diodes (LEDs) on the growth, antioxidant activities, and metabolites in adventitious root of Panax ginseng CA Meyer. In: Dutta Gupta S (ed) Light emitting diodes for agriculture. Springer, Singapore, pp 259–272
• Hahn EJ, Kozai T, Paek KY (2000) Blue and red light-emitting diodes with or without sucrose and ventilation affect in vitro growth of Rehmannia glutinosa plantlets. J Plant Biol 43:247–250
• Heo J, Lee C, Chakrabarty D, Paek K (2002) Growth responses of marigold and salvia bedding plants as affected by monochromic or mixture radiation provided by a light-emitting diode (LED). Plant Growth Regul 38:225–230
• Hung CD, Trueman SJ (2012) Alginate encapsulation of shoot tips and nodal segments for short-term storage and distribution of the eucalypt Corymbia torelliana × C. citriodora. Acta Physiol Plant 34:117–128
• Hung CD, Hong CH, Jung HB, Kim SK, Van Ket N, Nam MW, Choi DH, Lee HI (2015) Growth and morphogenesis of encapsulated strawberry shoot tips under mixed LEDs. Sci Hortic 194:194–200
• Jung ES, Lee S, Lim SH, Ha SH, Liu KH, Lee CH (2013) Metabolite profiling of the short-term responses of rice leaves (Oryza sativa cv. Ilmi) cultivated under different LED lights and its correlations with antioxidant activities. Plant Sci 210:61–69
• Kamiya A, Ikegami I, Hase E (1983) Effects of light on chlorophyll formation in cultured tobacco cells II. Blue light effect on 5-aminolevulinic acid formation. Plant Cell Physiol 24:799–809
• Karataş M, Aasim M, Dazkirli M (2016) Influence of light-emitting diodes and benzylaminopurin on adventitious shoot regeneration of water hyssop (Bacopa monnieri (L.) Pennell) in vitro. Arch Biol Sci 68:501–508
• Kim SJ, Hahn EJ, Heo JW, Paek KY (2004) Effects of LEDs on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro. Sci Hortic 101:143–151
• Kurilčik A, Miklušytė-Čanova R, Dapkūnienė S, Žilinskaitė S, Kurilčik G, Tamulaitis G, Duchovskis P, Žukauskas A (2008) In vitro culture of Chrysanthemum plantlets using light-emitting diodes. Cent Eur J Biol 3:161–167
• Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol 148:350–382
• Lin JY, Tang CY (2007) Determination of total phenolic and flavonoid contents in selected fruits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation. Food Chem 101:140–147
• Lin Y, Li J, Li B, He T, Chun Z (2011) Effects of light quality on growth and development of protocorm-like bodies of Dendrobium officinale in vitro. Plant Cell Tissue Organ Cult 105:329–335
• Liu YJ, Tong YP, Zhu YG, Ding H, Smith FA (2006) Leaf chlorophyll readings as an indicator for spinach yield and nutritional quality with different nitrogen fertilizer applications. J Plant Nutr 29:1207–1217
• Ma X, Wang Y, Liu M, Xu J, Xu Z (2015) Effects of green and red lights on the growth and morphogenesis of potato (Solanum tuberosum L.) plantlets in vitro. Sci Hortic 190:104–109
• Manivannan A, Soundararajan P, Halimah N, Ko CH, Jeong BR (2015) Blue LED light enhances growth, phytochemical contents, and antioxidant enzyme activities of Rehmannia glutinosa cultured in vitro. Hortic Environ Biotechnol 56:105–113
• Mengxi L, Zhigang X, Yang Y, Yijie F (2011) Effects of different spectral lights on Oncidium PLBs induction, proliferation, and plant regeneration. Plant Cell Tissue Org Cult 106:1–10
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497
• Nagesh KS, Shanthamma C, Bhagyalakshmi N (2009) Role of polarity in de novo shoot bud initiation from stem disc explants of Curculigo orchioides Gaertn. and its encapsulation and storability. Acta Physiol Plant 31:699–704
• Nhut DT, Huy NP, Tai NT, Nam NB, Luan VQ, Hien VT, Tung HT, Vinh BT, Luan TC (2015) Light-emitting diodes and their potential in callus growth, plantlet development and saponin accumulation during somatic embryogenesis of Panax vietnamensis Ha et. Grushv. Biotechnol Biotechnol Equip 29:299–308
• Nie Y, Dong X, He Y, Yuan T, Han T, Rahman K, Qin L, Zhang Q (2013) Medicinal plants of genus Curculigo: traditional uses and a phytochemical and ethnopharmacological review. J Ethnopharmacol 147:547–563
• Nishikimi M, Rao NA, Yagi K (1972) The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem Biophys Res Commun 46:849–854
• Pawłowska B, Żupnik M, Szewczyk-Taranek B, Cioć M (2018) Impact of LED light sources on morphogenesis and levels of photosynthetic pigments in Gerbera jamesonii grown in vitro. Hort Environ Biotechnol 59:115–123
• Poudel PR, Kataoka I, Mochioka R (2008) Effect of red- and blue-light-emitting diodes on growth and morphogenesis of grapes. Plant Cell Tissue Organ Cult 92:147–153
• Redenbaugh K, Fujii JA, Slade D (1991) Synthetic seed technology. In: Vasil IK (ed) Scale-up and automation in plant propagation. Academic Press, San Diego, pp 35–74
• Ren J, Guo S, Xu C, Yang C, Ai W, Tang Y, Qin L (2014) Effects of different carbon dioxide and LED lighting levels on the anti- oxidative capabilities of Gynura bicolor DC. Adv Space Res 53:353–366
• Rihan HZ, Kareem F, El-Mahrouk ME, Fuller MP (2017) Artificial seeds (principle, aspects and applications). Agron 7:71
• Samuolienė G, Brazaitytė A, Vaštakaitė V (2017) Light-emitting diodes (LEDs) for improved nutritional quality. In: Dutta Gupta S (ed) Light emitting diodes for agriculture. Springer, Singapore, pp 149–190
• Sharma S, Shahzad A, da Silva JAT (2013) Synseed technology - a complete synthesis. Biotechnol Adv 31:186–207
• Viršilė A, Olle M, Duchovskis P (2017) LED lighting in horticulture. In: Dutta Gupta S (ed) Light emitting diodes for agriculture. Springer, Singapore, pp 113–147
• Wang H, Gu M, Cui J, Shi K, Zhou Y, Yu J (2009) Effects of light quality on CO2 assimilation, chlorophyll-fluorescence quenching, expression of Calvin cycle genes and carbohydrate accumulation in Cucumis sativus. J Photochem Photobiol B: Biol 96:30–37
• Yeh N, Chung JP (2009) High-brightness LEDs—energy efficient lighting sources and their potential in indoor plant cultivation. Renew Sustain Energy Rev 13:2175–2180
• Zhishen J, Mengcheng T, Jianming W (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64:555–559

Identyfikatory

DOI

10.1007/s11738-019-2840-y