Anandamide in the anterior hypothalamus diminishes defensive responses elicited in mice threatened by Epicrates cenchria constrictor serpents

• Autorzy: Anjos-Garcia T. , Coimbra N.C.
• Języki publikacji: EN

Abstrakty

EN

The purpose of this study was to investigate whether the panicolytic‑like effect of different doses of anandamide microinjected into the anterior hypothalamus (AH) follows the same pattern of a bell‑shaped dose‑response curve observed with the same dose treatment in dorsomedial and ventromedial hypothalamus. We investigated this assumption by administering the cannabinoid and vanilloid receptor agonist anandamide into the anterior hypothalamus of mice and exposing them to the real threatening situation by using our experimental model based on confrontations between rodents and wild snakes. Our findings showed a gradual decay of response, with a significant attenuation of the panic attack‑like responses with anandamide at the highest dose but no effect was found after anandamide at the lowest or intermediate doses. An immunohistochemical procedure showed a lower degree of TRPV1 receptor and moderate to higher degree of Cb1 receptors in anterior hypothalamus. In conclusion, the pattern of dose‑response curve of anandamide microinjected in the AH does not seem to be the same classical pattern compared with other hypothalamic nuclei.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2020
• Tom: 80
• Numer: 2
• Opis fizyczny: p.179-191,fig.,ref.

Twórcy

autor

Anjos-Garcia T.

• Laboratory of Neuroanatomy and Neuropsychobiology, Department of Pharmacology, Ribeirao Preto Medical School, University of Sao Paulo (FMRP‑USP), Sao Paulo, Brazil
• NAP‑USP‑Neurobiology of Emotions Research Centre (NuPNE), Ribeirao Preto Medical School, University of Sao Paulo (FMRP‑USP), Sao Paulo, Brazil
• Ophidiarium LNN‑FMRP‑USP/INeC, Ribeirao Preto School of Medicine, University of Sao Paulo (FMRP‑USP), Sao Paulo, Brazil

autor

Coimbra N.C.

• Laboratory of Neuroanatomy and Neuropsychobiology, Department of Pharmacology, Ribeirao Preto Medical School, University of Sao Paulo (FMRP‑USP), Sao Paulo, Brazil
• NAP‑USP‑Neurobiology of Emotions Research Centre (NuPNE), Ribeirao Preto Medical School, University of Sao Paulo (FMRP‑USP), Sao Paulo, Brazil
• Behavioural Neuroscience Institute (INeC), Sao Paulo, Brazil
• Ophidiarium LNN‑FMRP‑USP/INeC, Ribeirao Preto School of Medicine, University of Sao Paulo (FMRP‑USP), Sao Paulo, Brazil

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Identyfikatory

DOI

10.21307/ane-2020-017