The effect of carbon source in culture medium on micropropagation of common ninebark (Physocarpus opulifolius (L.) Maxim.) ‘Diable D’or'

• Autorzy: Ilczuk A. , Jagiello-Kubiec K. , Jacygrad E.

Warianty tytułu

PL

Wpływ rodzaju cukru w pożywce na mikrorozmnażanie pęcherznicy kalinolistnej (Physocarpus opulifolius (L.) Maxim.) ‘Diable D’or’

• Języki publikacji: EN

Abstrakty

EN

Efficient plant micropropagation depends upon a number of factors one of which is the type and concentration of exogenously supplied carbon sources in the medium. This study tested several different sugars as carbon source on the efficiency of shoot proliferation and in vitro rooting of common ninebark (Physocarpus opulifolius (L.) Maxim.). Fructose, glucose, maltose and sucrose were tested at concentration ranging from 0–50 g dm-3. The dry matter content, reducing sugars and sucrose in shoots were related to sugar concentrations in the medium and so was the rate of adventitious root formation. Sucrose did not stimulate shoot proliferation and glucose was completely ineffective in rooting induction. The highest number of shoots was produced on the fructose-containing medium. The concentration of 30 g dm-3 appeared to be optimal; the rate of proliferation at 30 and 40 g dm-3 were in fact similar, but the former produced a more favorable shoot length. The number of adventitious roots produced per shoot increased with increasing fructose concentration up to 30 g dm-3. Fructose can be therefore recommended as the best C-source for the in vitro shoot proliferation and rooting in common ninebark.

PL

Wydajność mikro rozmnażania roślin uzależniona jest od wielu czynników, m.in. od koncentracji i rodzaju węglowodanów dodawanych do pożywki. Celem pracy było określenie wpływu różnych źródeł węgla na poziom proliferacji (współczynnik rozmnażania) i stopień ukorzenienia mikro sadzonek pęcherznicy kalinolistnej (Physocarpus opulifolius (L.) Maxim.) w warunkach in vitro. Do pożywek dodawano fruktozę, glukozę, maltozę i sacharozę w stężeniach 0–50 g dm-3. Zawartość suchej masy, cukrów redukujących i sacharozy w pędach związana była ze stężeniem węglowodanów w pożywce i wpływała na proces rizogenezy u mikro sadzonek. Sacharoza nie wpływała stymulująco na proliferację nowych pędów, natomiast glukoza okazała się być całkowicie nieefektywna w ukorzenianiu mikro sadzonek. Najwyższy współczynnik rozmnażania zaobserwowano na pożywce wzbogaconej we fruktozę. Pomimo że współczynnik rozmnażania na pożywce zawierającej 30 i 40 g dm-3 był zbliżony, to długość wytworzonych pędów była istotnie większa na pożywce zawierającej 30 g dm-3, dlatego stężenie to uznano za optymalne. Najwyższy stopień ukorzenienia mikro sadzonek zaobserwowano na pożywce z dodatkiem 30 g dm-3 fruktozy. Fruktoza może być więc zalecana jako najlepsze źródło węgla do namnażania i ukorzeniania pęcherznicy kalinolistnej w warunkach in vitro.

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2013
• Tom: 12
• Numer: 3
• Opis fizyczny: p.23-33,fig.,ref.

Twórcy

autor

Ilczuk A.

• Department of Ornamental Plants, Warsaw University of Life Sciences – SGGW, ul.Nowoursynowska 159, 02-776 Warsaw, Poland

autor

Jagiello-Kubiec K.

• Department of Ornamental Plants, Warsaw University of Life Sciences – SGGW, ul.Nowoursynowska 159, 02-776 Warsaw, Poland

autor

Jacygrad E.

• Department of Ornamental Plants, Warsaw University of Life Sciences – SGGW, ul.Nowoursynowska 159, 02-776 Warsaw, Poland

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