Determination of Cryptosporidium spp. in Van cats by nested PCR

• Autorzy: Orunc Kilinc O. , Yilmaz A.B. , Goz Y. , Ozkan C. , Denizhan V.
• Języki publikacji: EN

Abstrakty

EN

The present study was conducted to investigate the presence of Cryptosporidium spp. agents in cats from the Turkish Van Cat Shelter at YüzüncüYıl University by a modified Ziehl-Neelsen staining method and nested PCR. Individual stool samples were obtained from 30 adult females, 30 adult males and 40 kittens – a total of 100 Van cats were analyzed in the study. A simplified formol-ether concentration method was applied to all samples. The samples were then examined microscopically by a modified Ziehl-Neelsen staining method. As a result of the staining, Cryptosporidium spp. oocysts were identified in stool samples of 3 kittens in the microscopic examination. After that, PCR and nested-PCR were conducted with suitable primers. Nested PCR identified 5 kittens (5%) as positive. As a result, it was concluded that nested PCR was a superior diagnostic method for Cryptosporidium diagnosis compared with staining methods and that infected cats could be a health hazard for other cats and individuals, since Cryptosporidium spp. agents infect via the faecal-oral route. Therefore, we believe it is necessary to raise the awareness of people in contact with cats.

Słowa kluczowe

EN

Cryptosporidium; parasite; determination; Van cat zob.Turkish Van cat; Turkish Van cat; nested polymerase chain reaction

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2018
• Tom: 74
• Numer: 07
• Opis fizyczny: p.456-459,fig.,ref.

Twórcy

autor

Orunc Kilinc O.

• Ozalp Vocational School, Van Yuzuncu Yil University, 65100, Van, Turkey

autor

Yilmaz A.B.

• School of Health, Van Yuzuncu Yil University, 65100,Van, Turkey

autor

Goz Y.

• School of Health, Van Yuzuncu Yil University, 65100,Van, Turkey

autor

Ozkan C.

• Department of Internal Diseases, Faculty of Veterinary Medicine, Van Yuzuncu Yil University, 65100, Van, Turkey

autor

Denizhan V.

• Ozalp Vocational School, Van Yuzuncu Yil University, 65100, Van, Turkey

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Identyfikatory

DOI

10.21521/mw.6008