Activation of phenylpropanoid pathway in legume plants exposed to heavy metals. Part I. Effects of cadmium and lead on phenylalanine ammonia-lyase gene expression, enzyme activity and lignin content

• Autorzy: Pawlak-Sprada S. , Arasimowicz-Jelonek M. , Podgorska M. , Deckert J.
• Języki publikacji: EN

Abstrakty

EN

Species-specific changes in expression of phenylalanine ammonia-lyase (PAL) and lignin content were detected in roots of soybean (Glycine max L.) and lupine (Lupinus luteus L.) seedlings treated with different concentrations of cadmium (Cd2+, 0-25 mg/l) or lead (Pb2+, 0-350 mg/l). The stimulatory effect of both metals was observed in mRNA coding for PAL in soybean. In the case of lupine, changes of PAL mRNA level were dependent on the metal used: Cd2+ caused a decrease, whereas Pb2+ an increase of PAL transcript level. The activity of PAL was enhanced in both plant species at higher metal concentrations (15-25 mg/l of Cd2+ or 150-350 mg/l of Pb2+); however it was not directly correlated with PAL mRNA. This suggests a transcriptional and posttranscriptional control of PAL expression under heavy metals stress. In soybean, Cd2+ or Pb2+ treatment increased lignin content, while in lupine the effect was opposite. The decreased lignin accumulation in lupine roots in response to heavy metals, despite an increased PAL activity, suggests that the activated phenylpropanoid pathway was involved in the synthesis of secondary metabolites other than lignin.

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2011
• Tom: 58
• Numer: 2
• Opis fizyczny: p.211-216,fig.,ref.

Twórcy

autor

Pawlak-Sprada S.

• Department of Plant Ecophysiology, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University, Poznan, Poland

autor

Arasimowicz-Jelonek M.

autor

Podgorska M.

autor

Deckert J.

Bibliografia

• Booker FL, Miller JE (1998) Phenylpropanoid metabolism and phenolic composition of soybean [Glycine max (L.) Merr] leaves following exposure to ozone. J Exp Bot 49: 1191-1202.
• Cabané M, Pireaux JC, Léger E, Weber E, Dizengremel P, Pollet B, Lapierre C (2004) Condensed lignins are synthesized in poplar leaves exposed to ozone. Plant Physiol 134: 586-594. 
• Cahill DM, McComb JA (1992) A comparison of changes in phenylalanine ammonia-lyase activity, lignin and phenolic synthesis in roots of Eucalyptus calophylla (field resistant) and E. marginata (susceptible) when infected with Phytophtora cinnamoni. Physiol Mol Plant Pathol 40: 315-332.
• Clemens S (2001) Molecular mechanisms of plant metal tolerance and homeostasis. Planta 212: 475-486. 
• Cobbett C, Goldsbrough P (2002) Phytochelatins and metallothioneins: roles in heavy metal detoxification and homeostasis. Annu Rev Plant Biol 53: 159-182. 
• Das P, Samantaray S, Rout GR (1997) Studies on cadmium toxicity in plants: A review. Environ Pollut 98: 29-36. 
• Deckert J (2005) Cadmium toxicity in plants: Is there any analogy to its carcinogenic effect in mammalian cells? BioMetals 18: 475-481. 
• Deckert J, Gwóźdź EA (1999) The effect of lead on cyclin expression in lupine roots. Acta Physiol Plant 21: 249-256.
• Diaz J, Bernal A, Pomar F, Merino F (2001) Induction of shikimate dehydrogenase and peroxidase in pepper (Capsicum annuum L.) seedling in response to copper stress and its relation to lignification. Plant Sci 161: 179-188.
• Dixon RA, Paiva NL (1995) Stress-induced phenylpropanoid metabolism. Plant Cell 7: 1085-1097. 
• Dixon RA, Achnine L, Kota P, Liu Ch-J, Reddy MSS, Wang L (2002) The phenylpropanoid pathway and plant defence - a genomics perspective. Mol Plant Pathol 3: 371-390. 
• Doster MA, Bostock PM (1988) Quantification of lignin formation in almond bark in response to wounding and infection by Phytophtotra species. Phytopathol 78: 473-477.
• Fusco N, Micheletto L, Dal Corso G, Borgato L, Furini A (2005) Identification of cadmium-regulated genes by cDNA-AFLP in the heavy metal accumulator Brassica juncea L. J Exp Bot 56: 3017-3027. 
• Gagnon H, Ibrahim RK (1997) Effects of various elicitors on the accumulation and secretion of isoflavonoids in white lupin. Phytochemistry 44: 1463-1467.
• Hahlbrock H, Scheel D (1989) Physiology and molecular biology of phenylpropanoid metabolism. Annu Rev Plant Physiol 62: 31-35.
• Janas KM, Cvikrova M, Pałagiewicz A, Szafrańska K, Posmyk MM (2002) Constitutive elevated accumulation of phenylpropanoids in soybean roots at low temperature. Plant Sci 163: 369-373.
• Jbir N, Chaib W, Amma S, Jemmali A, Ayadi A (2001) Root growth and lignification of two wheat species differing in their sensitivy to NaCl, in response to salt stress. C R Acad Sci III 324: 863-868. 
• Jouili H, Ferjani EE (2003) Changes in antioxidant and lignifying enzyme activities in sunflower roots (Heliantthus annus L.) stressed with copper excess. C R Biologies 326: 639-644. 
• Kovacik J, Klejdus B (2008) Dynamics of phenolic acids and lignin accumulation in metal-treated Matricardia chamomilla roots. Plant Cell Rep 27: 605-615. 
• Lee B-R, Kim K-Y, Jung W-J, Avice J-C, Ourry A, Kim T-H (2007) Peroxidases and lignification in relation to the intensity of water-deficit stress in white clover (Trifolium repens L.) J Exp Bot 58: 1271-1279.  
• Lin Ch-Ch, Chen L-M, Liu Z-H (2005) Rapid effect of copper on lignin biosynthesis in soybean roots. Plant Sci 168: 855-861.
• Mandre M (2002) Relationships between lignin and nutrients in Picea abies L. under alkaline air pollution. Water Air Soil Poll 133: 361-377.
• Mithöfer A, Schulze B, Boland W (2004) Biotic and heavy metal stress response in plants: evidence for common signals. FEBS Lett 566: 1-5. 
• Patra M, Bhowmik N, Bandopadhyay B, Sharma A (2004) Comparison of mercury, lead and arsenic with respect to genotoxic effects on plant systems and the development of genetic tolerance. Environ Exp Bot 52: 199-223.
• Pawlak S, Firych A, Rymer K, Deckert J (2009) Cu,Zn-superoxide dismutase is differently regulated by cadmium and lead in roots of soybean seedlings. Acta Physiol Plant 31: 741-747.
• Pawlak-Sprada S, Stobiecki M, Deckert J (2011) Activation of phenylpropanoid pathway in legume plants expose to heavy metals: Part II. Profiling of isoflavonoids and their glycoconjugates induced in roots of lupine (Lupinus luteus) seedlings treated with cadmim and lead. Acta Biochim Pol 58: 217-223. 
• Przymusiński R, Gwóźdź EA (1999) Heavy metal-induced polypeptides in lupin roots are similar to pathogenesis-related proteins. J Plant Physiol 154: 703-708.
• Roth U, von Roepenack-Lahaye E, Clemens S (2006) Proteome changes in Arabidopsis thaliana roots upon exposure to Cd2+. J Exp Bot 57: 4003-4013. 
• Rucińska R, Waplak S, Gwóźdź EA (1999) Free radical formation and activity of antioxidant enzymes in lupine roots exposed to lead. Plant Physiol Biochem 37: 187-194.
• Rucińska R, Sobkowiak R, Gwóźdź EA (2004) Genotoxicity of lead in lupin root cells as evaluated by the comet assay. Cell Mol Biol Lett 9: 519-528. 
• Salman A, Goupil P, Filgueiras H (2008) Controlled atmosphere and heat shock affect PAL1 and HSP90 mRNA accumulation in fresh-cut endive (Cichorium intybus L.). Eur Food Res Technology 227: 721-726.
• Sandalio LM, Dalurzo HC, Gomez M, Romero-Puertas MC, del Rio LA (2001) Cadmium-induced changes in the growth and oxidative metabolism of pea plant. J Exp Bot 52: 2115-2126. 
• Sarma AD, Sharma R (1999) Purification and characterization of UV-B-induced phenylalanine ammonia-lyase from rice seedlings. Phytochemistry 50: 729-737.
• Seregin IV, Ivanov VB (2001) Physiological aspects of cadmium and lead toxic effects on higher plants. Russ J Plant Physiol 48: 523-544.
• Sharma P, Dubey RS (2005) Lead toxicity in plants. Braz J Plant Physiol 17: 35-52.
• Shingu Y, Kudo T, Ohsato S, Kimura M, Ono Y, Yamaguchi I, Hamamoto H (2005) Characterization of genes encoding metal tolerance proteins isolated from Nicotiana glauca and Nicotiana tabacum. Biochem Biophys Res Comm 331: 675-680. 
• Singh K, Kumar S, Rani A, Gulati A, Ahuja PS (2009) Phenylalanine ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H) and catechins (flavan-3-ols) accumulation in tea. Funct Integr Genomics 9: 125-134. 
• Sobkowiak R, Deckert J (2003) Cadmium-induced changes in growth and cell cycle gene expression in suspension-culture cells of soybean. Plant Physiol Biochem 41: 767-772.
• Sobkowiak R, Deckert J (2004) The effect of cadmium on cell cycle control in suspension culture cells of soybean. Acta Physiol Plant 26: 335-344.
• Sobkowiak R, Deckert J (2006) Proteins induced by cadmium in soybean cells. J Plant Physiol 163: 1203-1206. 
• Sobkowiak R, Rymer K, Rucińska R, Deckert J (2004) Cadmium-induced changes in antioxidant in suspension culture of soybean cells. Acta Biochim Pol 51: 219-222. 
• Srivastava AK, Venkatachalam P, Raghothama KG, Sahi SV (2007) Identification of lead-regulated genes by suppression subtractive hybridization in the heavy metal accumulator Sesbania drummondii. Planta 225: 1353-1365. 
• Taiz L, Zeiger E (2006) Plant Physiology. 4th edn, pp 315-341. Sinauer Associates, Inc. Publisher, Sunderland, Massachusetts, USA.
• Winkel-Shirley B (2002) Biosynthesis of flavonoids and effects of stress. Curr Opin Plant Biol 5: 218-223. 
• Yang Y-J, Cheng L-M, Liu Z-H (2007) Rapid effect of cadmium on lignin biosynthesis in soybean roots. Plant Sci 172: 632-637.

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