Resveratrol and nitric oxide cholinergic neurotoxity

• Autorzy: Gul-Hinc S. , Bielarczyk H. , Jankowska-Kulawy A. , Szutowicz A.
• Języki publikacji: EN

Abstrakty

EN

Several scavengers of free radicals like resveratrol (RV) are claimed to be useful in complementary therapy of Alzheimer’s dementia (AD) and other cholinergic encephalopathies, through the protection of mitochondrial energy metabolism. NO excess is one of recognized pathogens in AD brains. Therefore, we investigated whether RV might overcome cytotoxic effects of NO excess on septal cholinergic neuroblastoma SN56 cells. Chronic, 16 h exposure of nondifferentiated SN56 cells (NC) to 0.2 mM sodium nitroprusside (SNP) caused 30 and 27% suppression of pyruvate dehydrogenase (PDH) activity and acetyl-CoA level and the increase of nonviable cells fraction to 30%. In cAMP/retinoic acid-differentiated cells (DC), similar 30% inhibition of PDH by SNP caused 50% suppression of acetyl-CoA content and 47% loss of cell viability. RV (0.005 mM) itself affected none of parameters, neither in NC nor in DC. It did not overcome SNP-evoked inhibition of PDH and suppression of acetyl-CoA content in NC and DC. Despite of that RV partially prevented SNP-induced increase of DC and NC mortality to 31 and 14%, respectively. These data demonstrate that greater cytotoxic effects of SNP in DC than in NC correlated with evoked acetyl-CoA defi cits that might be linked with higher level of acetylcholine metabolism in the former. Protective effects of RV in NO-challenged cholinergic neurons were not connected with improvement of their acetyl-CoA metabolism. Supported by MNiSW projects NN401 2333 33, P05A 11030.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 3
• Opis fizyczny: p.341

Twórcy

autor

Gul-Hinc S.

• Department of Laboratory Medicine, Medical University of Gdansk, Gdansk, Poland

autor

Bielarczyk H.

• Department of Laboratory Medicine, Medical University of Gdansk, Gdansk, Poland

autor

Jankowska-Kulawy A.

• Department of Laboratory Medicine, Medical University of Gdansk, Gdansk, Poland

autor

Szutowicz A.

• Department of Laboratory Medicine, Medical University of Gdansk, Gdansk, Poland