Proteomic analysis of plasma profiles in children with recurrent bone fractures

• Autorzy: Rusinska A. , Swiatkowska M. , Koziolkiewicz W. , Skurzynski S. , Golec J. , Chlebna-Sokol D.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study is proteomic analysis of the plasma profile in children with recurrent bone fractures. The study involved 16 children: 6 patients with recurrent low-energy fractures and normal bone mass and 10 with osteogenesis imperfecta. In the analysis of the protein profile, the two-dimensional protein electrophoresis was used (Ettan DALT II, Amersham Bioscience). The images of protein gels were compared with controls. The protein spots with changed expression were cut from the gel and the amino acid sequence was analyzed with the mass spectrometry method (Q-Tof PremierTM API MASS SPECTROMETR, Waters) for protein identification. The most prevalent protein with changed expression, with respect to controls, was haptoglobin observed in 6 patients with a severe form of osteogenesis imperfecta. Increased haptoglobin concentration in these patients was confirmed by the ELISA method. Peptides corresponding to alpha-1 acid glycoprotein and serum amyloid P-component, apolipoprotein A-I, and transthyretin were detected in one, two and three children, respectively. Conclusions: 1) The results show increased haptoglobin which may be suggestive of an inflammatory component taking part in the course of osteogenesis imperfecta. 2) Further studies to explain the possible relationship of this protein with increased bone fragility are necessary.

Słowa kluczowe

EN

proteomic analysis; plasma profile; child; bone fracture; osteogenesis imperfecta; osteogenesis imperfecta type I; osteogenesis imperfecta type III; pediatrics; amino acid sequence; protein profile; two-dimensional electrophoresis; protein gel; protein identification

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2011
• Tom: 58
• Numer: 4
• Opis fizyczny: p.553-561,fig.,ref.

Twórcy

autor

Rusinska A.

• Department of Paediatric Propedeutics and Bone Metabolic Diseases, Medical University of Lodz, Lodz, Poland

autor

Swiatkowska M.

autor

Koziolkiewicz W.

autor

Skurzynski S.

autor

Golec J.

autor

Chlebna-Sokol D.

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