Expression and self-assembly of virus-like particles from porcine parvovirus and its application in antibody detection

• Autorzy: Li Y. , Wang Q. , Yue W. , Li X. , Chen Y. , Gao Y.
• Języki publikacji: EN

Abstrakty

EN

Porcine parvovirus (PPV) is a major causative agent in reproductive pig disease. The swine industry faces a significant economic and epizootic threat; thus, finding a reliable, quick, and practical way to detect it is essential. In this investigation, recombinant PPV VP2 protein was expressed in the Escherichia coli (E. coli) expression systems. As shown by electron microscopy (TEM), Western blot, and hemagglutination (HA) assays, the recombinant VP2 protein was successfully assembled into virus-like particles (VLPs) after being expressed and purified. These VLPs had a structure that was similar to that of real PPV viruses and also exhibited HA activity. These VLPs induced high levels of PPV-specific antibody titers in mice after immunization, indicating that the VLPs may be beneficial as potential candidate antigens. VLPs were used as the coating antigens for the VLP ELISA, and the PPV VLPs-based ELISA displayed a high sensitivity (99%), specificity (93.0%) and agreement rate (98.3%) compared to HI assay, and the agreement rate of this ELISA was 97.5% compared to a commercial ELISA kit. Within a plate, the coefficient of variation (CV) was 10%, and between ELISA plates, the CV was 15%. According to a cross-reactivity assay, the technique was PPV-specific in contrast to other viral illness sera. The PPV VLP indirect-ELISA test for PPV detection in pigs with an inactivated vaccine showed that the PPV-positive rate varied among different sample sources from 88.2 to 89.6%. Our results indicate that this ELISA technique was quick, accurate, and repeatable and may be used for extensive serological research on PPV antibodies in pigs.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2023
• Tom: 26
• Numer: 4
• Opis fizyczny: p.611-620,fig.,ref.

Twórcy

autor

Li Y.

• Beijing Biomedicine Technology Center of JoFunHwa Biotechnology (Nanjing Co. Ltd.); No.25 Xiangrui Street Daxing District, Beijing 102600 China

autor

Wang Q.

• Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China

autor

Yue W.

• Beijing Biomedicine Technology Center of JoFunHwa Biotechnology (Nanjing Co. Ltd.); No.25 Xiangrui Street Daxing District, Beijing 102600 China

autor

Li X.

• Beijing Biomedicine Technology Center of JoFunHwa Biotechnology (Nanjing Co. Ltd.); No.25 Xiangrui Street Daxing District, Beijing 102600 China

autor

Chen Y.

• Beijing Biomedicine Technology Center of JoFunHwa Biotechnology (Nanjing Co. Ltd.); No.25 Xiangrui Street Daxing District, Beijing 102600 China

autor

Gao Y.

• Beijing Biomedicine Technology Center of JoFunHwa Biotechnology (Nanjing Co. Ltd.); No.25 Xiangrui Street Daxing District, Beijing 102600 China

Identyfikatory

DOI

10.24425/pjvs.2023.148280