Screening of different extracts of marine macro green algae for larvicidal activity against dengue fever mosquito, Aedes aegypti (diptera: Culiadae)

• Autorzy: Adaikala Raj G. , Jayaraman M. , Krishnamoorthy S. , Chandrasekaran M. , Venkatesalu V.
• Języki publikacji: EN

Abstrakty

EN

The present study larvicidal activities of hexane, chloroform, ethyl acetate, acetone and methanol extracts of Halimeda macroloba, Decsne, Caulerpa racemosa (Frosk) Weber-Van-Bosses and Ulva lactuca Lin, (Chlorophyceae) against Aedes aegypti. The marine macro green algae extracts were tested against early 4 th instar larvae of A. aegypti using WHO protocol and concentrations of 200, 400, 600,800 and 1000 ppm. The observed mortality was made 24 and 48 h after treatment, data was subjected to probit analysis to determine the lethal concentration (LC₅₀ and LC₉₀) of the treated larvae of the tested species. Among the tested extracts the maximum efficacy was observed in the ethyl acetate extracts. The ethyl acetate extracts of the seaweeds showed the presence of terpenoids, tannins and phenolic compounds stronger than the other extracts. The results revealed that all the extracts showed varied levels of larvicidal activity against A. aegypti tested. However, the ethyl acetate extract of C. racemosa showed remarkable larvicidal activity against A. aegypti (LC₅₀=579.9 and LC₉₀=1255.4 ppm values at 24 h and LC₅₀=495.4 and LC₉₀=1073.9 ppm at 48 h) followed by U. lactuca (LC₅₀=588.1 and LC₉₀=1290.7 ppm values at 24 h, and LC₅₀= 530.8, and LC₉₀= 1160.0 ppm at 48 h), respectively. The lowest larval mortality was observed with hexane extract of H. macroloba against A. aegypti with values of LC₅₀=1116.8 and LC₉₀= 1824.5 ppm (after 24 h) and LC₅₀=1059.9 and LC₉₀=1768.3 ppm (after 24 h). The present studies indicate that the larvicidal activity and phytochemicals derived from the ethyl acetate extract of C. racemosa have the potential to be used as an ideal eco-friendly approach and effective mosquito vector control agent.

Słowa kluczowe

EN

marine alga; green alga; algal extract; larvicidal activity; Aedes aegypti; dengue virus; phytochemical analysis; screening

• Wydawca: -
• Czasopismo: International Letters of Natural Sciences
• Rocznik: 2017
• Tom: 62
• Opis fizyczny: p.44-51,ref.

Twórcy

autor

Adaikala Raj G.

• Department of Botany, Annamalai University, Annamalainagar - 608 002, Tamil Nadu, India

autor

Jayaraman M.

• Department of Botany, Annamalai University, Annamalainagar - 608 002, Tamil Nadu, India

autor

Krishnamoorthy S.

• Department of Botany, Annamalai University, Annamalainagar - 608 002, Tamil Nadu, India

autor

Chandrasekaran M.

• Department of Botany, Annamalai University, Annamalainagar - 608 002, Tamil Nadu, India

autor

Venkatesalu V.

• Department of Botany, Annamalai University, Annamalainagar - 608 002, Tamil Nadu, India

Bibliografia

• [1] P. Bhadury, C.P. Wright, Exploitation of marine algae: biogenic compounds for potential antifouling application, In Planta. 219 (2004) 561-578.
• [2] V. Dhargalkar, K. Neelam Pereira, Seaweed: Promising plant of the Millennium. Sci. Cul. 71 (2005) 60-66.
• [3] E. Sandsdalen et al., The antibacterial effect of a polyhydroxylated fucophlorethol from the marine brown alga, Fucus vesiculosus, World. J. Microbiol. Biotechnol. 19 (2003) 777-782.
• [4] World Health Organization, A global brief on vector-borne diseases. WHO/DCO/WHD/2014; 1.
• [5] L. Roberts, Mosquitoes and disease, Sci. 298 (2002) 82–83.
• [6] World Health Organization, Dengue and dengue haemorrhagic fever. Fact sheet 2002; 117.
• [7] K. Samidurai et al., Larvicidal, ovicidal and repellent activities of Pemphis acidula forst (Lythraceae) against filarial and dengue vector mosquitoes, Acada. J. Entomol. 2(2) (2009) 62-66.
• [8] A. Manilal et al., Biopotentials of seaweeds collected from southwest coast of India, J. Mar. Sci. Technol. 17 (2009) 67–73.
• [9] I.B. Harborne, Phytochemical methods: A guide to modern techniques of plant analysis, 2nd ed., Chapman and Hall, New York, 1973, pp. 88-185.
• [10] World Health Organization. Instruction for determining the susceptibility or resistance of mosquito larvae to insecticides. WHO-VBC 81.807 (1981) 1–6.
• [11] M. Govindarajan, A. Jebanesan, T. Pushpanathan, Larvicidal and ovicidal activity of Cassia fistula Linn. leaf extract against filarial and malarial vector mosquitoes, Parasitol. Res. 102 (2008) 289-292.
• [12] M.P. Isman, Pesticides based on plant essential oils for management of plant pests and disease. In: International Symposium on Development of Natural Pesticides from Forest Resources. Seoul: Korea Forest Research Institute, 2001, pp. 1-9.
• [13] M.W. Service, Mosquitoes (Culicidae), in: R.P. Lane, R.W. Crosskey (Eds.), Medical insects and arachnids. Chapman and Hall, London, 1993, pp. 723-725.
• [14] M.Y.S. Ali, S. Ravikumar, J.M. Beula, Mosquito larvicidal activity of seaweeds extracts against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus, Asian Pac. J. Trop. Dis. 3 (2013) 196-201.
• [15] A. Manilal et al., Biological activity of the red alga Laurencia brandenii, Acta Bot. Croat. 70 (2011) 81-90.
• [16] T.S. Thangam, K. Kathiresan, Mosquito larvicidal effect of seaweed extracts, Bot. Mar. 34 (1991) 433–5.
• [17] S. Ravikumar et al., Antiplasmodial activity of two marine polyherbal preparations from Chaetomorpha antennina and Aegiceras corniculatum against Plasmodium falciparum, Parasitol. Res. 108 (2010) 107-13.
• [18] G. Adaikalaraj et al., Phytochemical profile and larvicidal properties of seed essential oil from Nigella sativa L. (Ranunculaceae), against Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus (Diptera: Culicidae), Parasitol Res. 114 (2015) 3385-3391.
• [19] S. Krishnamoorthy, G. Adaikala Raj, M. Chandrasekaran, Antibacterial and antifungal activity of leaves of Thespesia populnea, Int. J. Pharm. Pharmace. Sci. 6(8) (2014) 404-411.
• [20] C.E. Chariandy et al., Screenig of medicinal plants from Trinidad and Tobago for antimicrobial and insecticidal properties, J. Ethnopharmacol. 64 (1999) 265–270.

Identyfikatory

DOI

10.18052/www.scipress.com/ILNS.62.44