Characterization of olive seed storage proteins

• Autorzy: Wang W. , de Dios Alche J. , Rodriguez-Garcia M. I.
• Języki publikacji: EN

Abstrakty

EN

At present little is known about olive seed storage proteins (SSPs). A better understanding of olive SSPs will be important for future biotechnology efforts. In the present study, we first developed a protocol relied on chloroform for preparing protein samples free of lipids from lipid-rich olive seeds. Then, we characterized olive SSPs by SDS-PAGE, N-terminal sequencing and immunoblot. Two smaller subunits (20 and 21.5 kD) of SSPs were purified to homogeneity and used for antibody production or N-terminal sequencing. N-terminal sequencing confirmed that major olive SSPs are 11S globulins. Moreover, the components and size distribution of SSPs are identical among several olive cultivars examined, suggesting that their synthesis is highly conserved in this species. Olive SSPs are soluble in aqueous alcohol, with limited solubility in water and dilute salt. Thus, despite their homology with globulins, olive SSPs are similar in solubility to prolamins and different from globulins in other dicot plants. Finally, the accumulation of olive SSPs during fruit maturation was examined. Our results revealed that the accumulation of SSPs is time-dependent and tissue-specific, and only 105 days after pollination (DAP), did individual components of SSPs synthesize substantially, and accumulate rapidly in large quantities over a short period of time. Our results suggest that a 36 kD protein is the precursor of olive SSPs, and 90–105 DAP seems to be a crucial transition period (from a precursor to mature subunits) for the accumulation of SSPs.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2007
• Tom: 29
• Numer: 5
• Opis fizyczny: p.439-444,fig.,ref.

Twórcy

autor

Wang W.

• College of Life Science, Henan Agricultural University, Zhengzhou 450002, China

autor

de Dios Alche J.

• Departamento de Bioquimica, Biologia Celuler y Molecular de Plantas, Estacion Experimental del Zaidin, CSIC, 18008 Granada, Spain

autor

Rodriguez-Garcia M. I.

• Departamento de Bioquimica, Biologia Celuler y Molecular de Plantas, Estacion Experimental del Zaidin, CSIC, 18008 Granada, Spain

Bibliografia

• Barranco D (2004) Variedades y patrones. In: Barranco D, Fernández-Escobar R, Mundi-Prensa (eds) El Cultivo del Olivo. Madrid, pp 63–91
• Beardmore T, Wetzel S, Burgess D, Charest PJ (1996) Characterization of seed storage proteins in populus and their homology with populus vegetative storage proteins. Tree Physiol 16:833–840
• Brand JA, Tsang VCW, Zhou W, Shukla SB (1990) Comparison of particulate 3,3',5,5'-tetramethylbenzidine and 3,3'-diaminobenzidine as chromogenic substrates for immunoblot. Biotechniques 8:58–60
• Dickinson CD, Hussein EHA, Nielsen NC (1989) Role of post-translational cleavage in glycinin assembly. Plant Cell 1:459–469
• Edman P (1950) Method for determination of amino acid sequence in peptides. Acta Chem Scand 4:83–293
• FAO (2004) Faostat statistical databases. http://www.apps.fao.org/page/collections [accessed 30 June 2006]
• Gifford DJ, Greenwood JS, Bewley JD (1982) Deposition of matrix and crystalloid storage proteins during protein body development in the endosperm of Ricinus communis L. cv. Hale seeds. Plant Physiol 69:1471–1478
• Hayashi M, Mori H, Nishimura M, Akazawa T, Hara-Nishimura I (1988) Nucleotide sequence of cloned cDNA coding for pumpkin 11-S globulin beta-subunit. Eur J Biochem 172:627–632
• Herman EM, Larkins B (1999) A. Protein storage bodies and vacuoles. Plant Cell 11:601–614
• Higgins TJV (1984) Synthesis and regulation of major proteins in seeds. Ann Rev Plant Physiol 35:191–221
• Hu B, Esen A (1981) Homogeneity of soybean seed proteins: one-dimensional electrophoresis studies of six different solubility fractions. J Agric Food Chem 29:497–501
• Kiritsakis AK (1990) History of the olive tree. In: Kiristsakis AK (ed) Olive oil. American Oil Chemists’ Society, Champaign, pp 1–8
• Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 222:680–685
• Lalonde L, Fountain DW, Kermode A, Ouellette FB, Scott K, Bewley JD, Gifford DJ (1984) A comparative study of the insoluble storage proteins and the lectins of seeds of the Euphobiaceae. Can J Bot 62:671–1677
• Osborne TB (1924) The vegetable proteins, 2nd edn. Longmans, Green
• Pearson WR (2000) Flexible sequence similarity searching with the FASTA3 program package. Methods Mol Biol 132:185–219
• Ross JHE, Sanchez J, Millan F, Murphy DJ (1993) Differential presence of oleosins in oleogenic seed and mesocarp tissue in olive (Olea europaea) and avocado (Persea americana). Plant Sci 93:203–210
• Salas JJ, Sanchez J (1998) Alcohol dehydrogenases from olive (olea europaea) fruit. Phytochem 48:35–40
• Sciancalepore V, Longone V (1984) Polyphenol oxidase activity and browning in green olives. J Agric Food Chem 32:320–321
• Shewry PR, Napier JA, Tatham AS (1995) Seed storage proteins: structures and biosynthesis. Plant Cell 7:945–956
• Shutuck-Edidens DM, Beachy RN (1985) Degradation of β-conglycinin in early stage of soybean embryogenesis. Plant Physiol 78:895–898
• Suda CNK, Giorgini JF (2000) Seed reserve composition and mobilization during germination and initial seedling development of Euphorbia heterophylla. R Bras Fisiol Veg 12:226–245
• Tai HH, Bush RS (1997) Analysis of lupin seed protein digestibility using gel electrophoresis and immunoblots. J Anim Sci 75:1934–1940
• Thomas TL (1993) Gene expression during embryogenesis and germination: an overview. Plant Cell 5:1401–1410
• Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354
• Wang W, Alché JD, Castro AJ, Rodríuez-García MI (2001) Characterization of seed storage proteins and their synthesis during seed development in Olea europaea. Int J Dev Biol 45(s1):63–64
• Zamora R, Alaiz M, Hidalgo FJ (2001) Influence of cultivar and fruit ripening on olive (Olea europaea) fruit protein content, composition, and antioxidant activity. J Agric Food Chem 49:4267–4270