Evaluation of bone marrow in female fallow deer kept in captivity

• Autorzy: Snarska A. , Pomianowski A. , Sobiech P. , Gonkowski S. , Lew M. , Drazek M. , Zarczynska K. , Wysocka D. , Rytel L. , Stopyra A.
• Języki publikacji: EN

Abstrakty

EN

Proper processes of hematopoiesis have a major impact on the results of blood morphological analyses in healthy animals. The aim of this study was to evaluate bone marrow smears of female fallow deer kept in captivity and comparing them to morphological analysis of peripheral blood. To achieve the aim of this study bone marrow was collected from 10 nonpregnant, 2-year-old female fallow deer. Smears stained by the May-Grünwald Giemsa method were analyzed. Bone marrow samples were collected using a biopsy needle from the 3rd-4th rib in the sternal region into test tubes without coagulant. Whole blood for hematological analysis was sampled from the external jugular vein into 2 ml test tubes containing K2 EDTA. The results of the research indicate significant morphological differences of white blood cells line and red blood cells line of fallow deer in comparison to other ruminants. Proerythroblasts of fallow deer appear to have a higher number of nuclei that stain more clearly in the MGG method. In red the blood cells line a high percentage of polychromatic erythroblasts (17.2%) and basophilic erythroblasts (8.19%) was noted. In granulocyte line only 0.97% of myeloblasts was observed in bone marrow smears. The percentage of segmented and band granulocytes was determined at 6.86% and 2.27% consequently. In the lymphoblastic line of fallow deer a low percentage of plazmocytes was observed (1.16%). In the platelets line the percentage of megakaryoblasts (5.85%) was lower than megakaryocytes (7.9%). This report is the first study concerning hematopoiesis in female fallow deer.

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2017
• Tom: 73
• Numer: 09
• Opis fizyczny: p.544-548,fig.,ref.

Twórcy

autor

Snarska A.

• Department of Internal Disease, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego St. 14, 10-717 Olsztyn, Poland

autor

Pomianowski A.

• Department of Internal Disease, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego St. 14, 10-717 Olsztyn, Poland

autor

Sobiech P.

• Department of Internal Disease, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego St. 14, 10-717 Olsztyn, Poland

autor

Gonkowski S.

• Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego St. 14, 10-717 Olsztyn, Poland

autor

Lew M.

• Department of Surgery and Rentgenology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego St. 14, 10-717 Olsztyn, Poland

autor

Drazek M.

• Department of Surgery and Rentgenology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego St. 14, 10-717 Olsztyn, Poland

autor

Zarczynska K.

• Department of Internal Disease, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego St. 14, 10-717 Olsztyn, Poland

autor

Wysocka D.

• Department of Internal Disease, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego St. 14, 10-717 Olsztyn, Poland

autor

Rytel L.

• Department of Internal Disease, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego St. 14, 10-717 Olsztyn, Poland

autor

Stopyra A.

• Department of Internal Disease, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego St. 14, 10-717 Olsztyn, Poland

Bibliografia

• Ban A., Ogata Y., Kato T.: Erythrocyte morphology and frequency of spherocytes in hereditary erythrocyte membrane protein disorder in Japanese Black cattle. Bull. Nippon Vet. Anim. Sci. Univ. 1995, 44, 21-27.
• Barić R. R., Tonćić R. J., Vicković I., Sostarić B.: Haematological and biochemical values of farmed red deer. Vet. Arhiv 2011, 81, 513-523.
• Burvenich C., Paape M. J., Hill A. W., Guidry A. J., Miller R. H., Heyneman R., Kremer W. D. J.: Role of neutrofil leucocyte in the local and systemic reactions during experimentally induced E. coli mastitis in cows immediately after calving. Vet. Quart. 1994, 16, 45-50.
• English A. W., Lepherd E. E.: The haemathology and serum biochemistry of wild fallow deer (Dama dama) in New South Wales. J. Wildlife Dis. 1991, 17, 289-395.
• Heynemann R., Burvenich C., Vecauteren R.: Interaction between the respiratory burst activity of neutrophil leukocytes and experimentally induced Escherichia coli mastitis in cows. J. Dairy Sci. 1990, 73, 985-994.
• Hoedemaker M., Lund L. A., Wagner W. C.: Influence of arachidonic-acid metabolites and steroids on function of bovine polymorphonuclear neutrophils. Vet. Res. 1992, 53, 1534-1539.
• Janicki Z., Forsek J., Slavica A., Kolić E., Dezdek D., Manojlović L.: Haematological values of fallow deer (Dama dama) population in continental and island habitats of Croatia. Vet. Arhiv 2000, 70 (Suppl.), 201-206.
• Keller S. L., Jefferson B. J., Jacobs R. M., Wood D.: Effect of noncytopathic type 2 Bovine viral diarrhea virus on the proliferation of bone marrow progenitor cells. Can. J. Vet. Res. 2000, 70, 20-27.
• Lin J., Linzer D. I.: Induction of megakaryocyte differentiation by a novel pregnancy-specific hormone. J. Biol. Chem. 1999, 274, 21485-21489.
• Marco I., Lavin S.: Effect of the method of capture on the haemathology and chemistry of red deer (Cervus elaphus). Res. Vet. Sci. 1999, 66, 81-84.
• Maryuama S., Minagawa M., Shimizu T., Oya H., Yamamoto S., Musha N., Abo W., Weerasinghe A., Hatakeyama K., Abo T.: Administration of glucocorticosteroids markedly increases the numbers of granulocytes and extrathymic T cells in the bone marrow. Cell. Imunol. 1999, 194, 28-35.
• McCrae K. R.: Thrombocytopenia in pregnancy: differential diagnosis, pathogenesis, and management. Blood Rev. 2003, 17, 7-14.
• Merris V., Meyer E., Duchateau L., Burvenich C.: Differential effects of steroid and retinoid on bovine myelopoiesis in vitro. J. Dairy Sci. 2004, 87, 1188-1195.
• Merris V., Van Meyer E., Dosogne H., Burvenich C.: Separation of bovine bone marrow into maturation-related cell fractions. Vet. Immunol. Immunopathol. 2001, 83, 11-17.
• Rosef O., Nystoyl H., Solenes T., Arnemo J. M.: Haematological and serum biochemical reference values in free ranging red deer (Cervus elaphus atlanticus). Rangifer 2004, 24, 79-85.
• Scruggs D. W., Fleming S. A., Maslin W. R., Groce A. W.: Osteopetrosis, anemia, thrombocytopenia, and marrow necrosis in beef-calves naturally infected with Bovine Virus Diarrhea Virus. J. Vet. Diagn. Invest. 1995, 7, 555-559.
• Snarska A., Krystkiewicz W., Rękawek W.: Assesment of leukocytes in the bone marrow of healthy Holstein- Friesian cows. Med. Weter. 2013, 69, 674-677.
• Snarska A., Krystkiewicz W., Sobiech P., Żarczyńska K., Rękawek W., Stopyra A.: Assesment of erythroblastic cells in the bone marrow of healthy Holstein- Friesian cows. Med. Weter. 2013, 69, 540-542.
• Snarska A., Krystkiewicz W., Żarczyńska K., Rękawek W., Sobiech P., Radwińska J.: Evaluation of bone marrow in Holstein-Fresian cattle infected with the BVDV virus. Med. Weter. 2013, 69, 187-190.
• Snarska A., Sobiech P.: Evaluation of bone marrow with particular consideration of the megakaryocyte lineage and coagulation profile in pregnant fallow deer (Dama dama). Pol. J. Vet. Sci. 2016, 19:359-364. DOI: 10.1515/pjvs-2016-0044
• Steffen D. J., Elliott G., Leipold H. W., Smith J. E.: Congenital dyserythtropoesis and progressive alopecia in Polled Hereford calves: hematologic, biochemical, bone marrow cytologic, electrophoretic, and flow cytometric findings. J. Vet. Diagn. Invest. 1992, 4, 31-37.
• Wardlaw A. J., Kay A. B.: Eosinophils: production, biochemistry and function. Williams Hematology, New York 1995, 798-805.

Identyfikatory

DOI

10.21521/mw.5773