Biological and molecular characterization of the Polish Zucchini yellow mosaic virus isolates

• Autorzy: Hasiow-Jaroszewska B. , Rymelska N. , Borodynko N. , Pospieszny H.

Warianty tytułu

PL

Biologiczna i molekularna charakterystyka polskich izolatów wirusa mozaiki cukinii (Zuchinii yellow mosaic virus)

• Języki publikacji: EN

Abstrakty

EN

The diversity of Zucchini yellow mosaic virus (ZYMV) isolates from cucumber and zucchini plants growing in different regions of Poland was analyzed using biological tests and molecular biology techniques. The isolates differed in their host range and symptoms induced by them on a series of plant species. In addition, the analysis of the genetic diversity of the coat protein (CP) gene revealed high level of nucleotide variability among the isolates. Comparison of the CP gene sequences of 70 isolates from different geographical regions worldwide showed that the Polish isolates belong to different groups and they do not form a monophyletic cluster with European isolates. Interestingly, among the central European ZYMV isolates lower variability has been observed previously. The ratio of nonsynonymous to synonymous polymorphic sites showed a dominant negative selection however codons which might undergo positive selection were also identified. Moreover, the evidences for recombination in analyzed sequences of the CP gene of the analyzed ZYMV isolates were provided.

PL

Wirus żółtej mozaiki cukinii (Zucchini yellow mosaic virus, ZYMV) charakteryzuje się wysokim stopniem zróżnicowania zarówno biologicznego, jak i genetycznego. W pracy analizowano zakres roślin gospodarzy i symptomy wywoływane przez polskie izolaty ZYMV. Ponadto przeprowadzono analizę filogenetyczną i przebiegu rekombinacji z wykorzystaniem sekwencji nukleotydów genu kodującego białko płaszcza polskich i 67 innych izolatów ZYMV, których sekwencje zdeponowano w Banku Genów. Badania wykazały, że polskie izolaty nie tylko różniły się od siebie w teście biologicznym, ale należą do różnych grup filogenetycznych, nie tworząc tym samym jednej grupy z innymi izolatami europejskimi. Analiza presji selekcyjnej działającej na populację izolatów ZYMV wykazać dominację presji negatywnej, aczkolwiek zidentyfikowano również kodony, które mogą podlegać wpływowi pozytywnej presji selekcyjnej.

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2013
• Tom: 12
• Numer: 2
• Opis fizyczny: p.75-85,fig.,ref.

Twórcy

autor

Hasiow-Jaroszewska B.

• Department of Virology and Bacteriology, Institute of Plant Protection - National Research Institute, Wl.Wegorka 20, 60-318 Poznan, Poland

autor

Rymelska N.

• Department of Virology and Bacteriology, Institute of Plant Protection - National Research Institute, Wl.Wegorka 20, 60-318 Poznan, Poland

autor

Borodynko N.

• Department of Virology and Bacteriology, Institute of Plant Protection - National Research Institute, Wl.Wegorka 20, 60-318 Poznan, Poland

autor

Pospieszny H.

• Department of Virology and Bacteriology, Institute of Plant Protection - National Research Institute, Wl.Wegorka 20, 60-318 Poznan, Poland

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