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2018 | 77 | 2 |

Tytuł artykułu

Immunohistochemical study on roe deer haemal nodes

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Background: The aim of this study was to investigate the immunohistochemical characteristics of the haemal nodes located in the abdominal and thoracic cavities in roe deer (Capreolus capreolus). Materials and methods: In this study, 2 adult male and 2 adult female roe deers in addition to 2 roe deer foetuses at the late foetal stages were used. The avidin-biotin-peroxidase technique was applied to anti-CD3, anti-CD79αcy, anti-macrophage, anti-S100 primary antibodies. Results: Positive results were gained for all the antibodies used. Many CD3 positive T-lymphocytes were seen in the lymphoid tissue areas of the foetal haemal nodes, in the germinal centre of the lymph follicles of the adult haemal nodes and in their lymphatic cords. CD79αcy positive cells were less in number while S-100 protein was positive in both lymph follicles and in sinuses of the adult haemal nodes. In foetal haemal nodes, cells positive for S-100 protein were more prevalent in the sinuses. For the MAC387 primary antibody, there were positive macrophages seen in the capsule and trabeculae of the haemal nodes, located denser in males. Macrophage cells in the lymphoid structures and granulocytes in the sinuses of the haemal nodes of both adults and foetuses were positive for MAC387. Conclusions: This study is the first one to define the detailed structural features immunohistochemically in the haemal nodes of the roe deer. Even it’s concluded that the results were similar with the other ruminants, it’s also pointed out that there are certain differences among the foetal, adult male and female haemal nodes of the roe deer. (Folia Morphol 2018; 77, 2: 266–271)

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Czasopismo

Rocznik

Tom

77

Numer

2

Opis fizyczny

p.266–271,fig.,ref.

Twórcy

autor
  • Department of Histology and Embryology, Faculty of Veterinary Medicine, Mustafa Kemal University, Hatay, Turkey
  • Department of Histology and Embryology, Faculty of Veterinary Medicine, Kafkas University, Kars, Turkey
autor
  • Department of Anatomy, Faculty of Veterinary Medicine, Ondokuz Mayıs University, Samsun, Turkey

Bibliografia

  • 1. Akaydin Bozkurt Y, Kabak M. Morphology of haemal nodes in the roe deer (Capreolus capreolus). Anat Histol Embryol. 2010; 39(5): 456–461, doi: 10.1111/j.1439-0264.2010.01016.x, indexed in Pubmed: 20624158.
  • 2. Bacha WJ, Bacha LM. Color Atlas of Veterinary Histology. Third ed. Wiley-Blackwell, A John Wiley&Sons, Inc, Publication, UK. 2012.
  • 3. Casteleyn CR, Breugelmans S, Simoens P, et al. Morphological and immunological characteristics of the bovine temporal lymph node and hemal node. Vet Immunol Immunopathol. 2008; 126(3-4): 339–350, doi: 10.1016/j.vetimm.2008.09.010, indexed in Pubmed: 18952301.
  • 4. Ceccarelli P, Gargiulo AM, Fagioli O, et al. Cytochemical identification of lymphocytes and other mononuclear cells in ovine and bovine hemal nodes. Comp Immunol Microbiol Infect Dis. 1986; 9(4): 297–302, indexed in Pubmed: 3026721.
  • 5. Cerutti P, Marcaccini A, Guerrero F. A Scanning and Immunohistochemical Study in Bovine Haemal Node. Anat Histol Embryol. 1998; 27(6): 387–392, doi: 10.1111/j.1439-0264.1998.tb00212.x.
  • 6. Cocchia D, Tiberio G, Santarelli R, et al. S-100 protein in “follicular dendritic” cells or rat lymphoid organs. An immunochemical and immunocytochemical study. Cell Tissue Res. 1983; 230(1): 95–103, indexed in Pubmed: 6342797.
  • 7. Dassanayake RP, Truscott TC, Özyiğit MÖ, et al. Accumulation profiles of PrP(Sc) in hemal nodes of naturally and experimentally scrapie-infected sheep. BMC Vet Res. 2013; 9: 82, doi: 10.1186/1746-6148-9-82, indexed in Pubmed: 23601183.
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  • 11. Guerrero F, Cerutti P, Marcaccini A, et al. Histological and immunohistochemical study on Iberian red deer (Cervus elaphus hispanicus) haemal nodes. Microsc Microanal. 2012; 18(Suppl 5): 1–2, doi: 10.1017/S1431927612012664.
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  • 13. Romero-Palomo F, Risalde MA, Molina V, et al. Immunohistochemical detection of dendritic cell markers in cattle. Vet Pathol. 2013; 50(6): 1099–1108, doi: 10.1177/0300985813482951, indexed in Pubmed: 23528943.
  • 14. Sugimura M, Shirogane D, Atoji Y, et al. A comparative study on S-100 protein-immunoreactive cells in lymph nodes. Nihon Juigaku Zasshi. 1990; 52(5): 1015–1021, indexed in Pubmed: 2280486.
  • 15. Sur E, Aydın MF, Çelik İA. Light microscopic investigation on the histology and Alpha-Naphthyl Acetate Esterase (ANAE)-positive lymphocyte localization in the hemal nodes of Akkaraman sheep. Eurasian J Vet Sci. 2005; 21(1-2): 101–108.
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Bibliografia

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