Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2014 | 36 | 01 |

Tytuł artykułu

The physiological mechanism of enhanced oxidizing capacity of rice (Oryza sativa L.) roots induced by phosphorus deficiency


Warianty tytułu

Języki publikacji



Plants show various responses to phosphorus (P) deficiency. Root oxidizing capacity enhancement is one of adaptive mechanisms for rice (Oryza sativa L.) to P deficiency. However, it remains unclear how P deficiency enhances the root oxidizing capacity. In this study, rice seedlings were treated in P-deficient nutrient solution for different periods. Variations of reactive oxygen species (ROS), antioxidant enzyme activity, root lignin content, root porosity, root oxygen release, total oxidative substances and root structural changes in rice roots in response to P-sufficient and P-deficient treatments were investigated. Results indicated that P deficiency induced the production of H₂O₂ and O₂ - in roots significantly, which reached their maximum after 1- to 2-day P-deficient treatment. Interestingly, the endogenous total oxidative substances kept stable in rice roots. P deficiency increased the activities of peroxidase and superoxide dismutase by 89.5 and 51.8 % after 4-day P-deficient treatment, respectively. Moreover, one-day P deficiency elevated lignin accumulation. Root porosity of rice seedling under 2-day P-deficient treatment was 19.8 % higher than that under P-sufficient treatment. P deficiency also enhanced the release of both O₂ and total oxidative substances after 1- to 4-day P deficiency. In addition, results from electronic microscopy indicated that the thickness of root cell wall tended to increase after 2-day P-deficient treatment. Taken together, our results suggested that P-deficiency-induced enhancement of root oxidizing capacity in rice roots was probably associated with ROS production, antioxidant enzyme activity increment in root tissues, and the release of O₂ and oxidative substances from root inside to rhizosphere.

Słowa kluczowe








Opis fizyczny



  • Laboratory of Rool Layer Control, College of Natural Resources and Environment, South China Agricultural University, No. 483, Wushan Roat, Guangzhou 510642, China
  • Laboratory of Rool Layer Control, College of Natural Resources and Environment, South China Agricultural University, No. 483, Wushan Roat, Guangzhou 510642, China
  • Laboratory of Rool Layer Control, College of Natural Resources and Environment, South China Agricultural University, No. 483, Wushan Roat, Guangzhou 510642, China


  • Almagro L, Gómez Ros LV, Belchi-Navarro S, Bru R, Ros Barceló A, Pedreño MA (2009) Class III peroxidases in plant defence reactions. J Exp Bot 60:377–390.
  • Amako K, Chen G, Asada K (1994) Separate assays specific for ascorbate peroxidase and guaiacol peroxidase and for the chloroplastic and cytosolic isozymes of ascorbate peroxidase in plants. Plant Cell Physiol 35:497–504.
  • Ando T, Yoshida S, Nishiyama I (1983) Nature of oxidizing power of rice roots. Plant Soil 72:57–71.
  • Armstrong W (1979) Aeration in higher plants. Adv Bot Res 7:225–332.
  • Asada K, Takahashi M (1987) Production and scavenging of active oxygen in photosynthesis. In: Kyle DJ, Osmond CB, Arntzen CJ (eds) Photoinhibition (topics in photosynthesis), vol 19. Elsevier, Amsterdam, pp 227–287.
  • Bargaz A, Faghire M, Farissi M, Drevon JJ, Ghoulam C (2013) Oxidative stress in the root nodules of phaseolus vulgaris is induced under conditions of phosphorus deficiency. Acta Physiol Plant 35(5):1633–1644.
  • Bouranis DL, Chorianopoulou SN, Kollias C, Maniou P, Protonotarios VE, Siyiannis VF, Hawkesford MJ (2006) Dynamics of aerenchyma distribution in the cortex of sulfate-deprived adventitious roots of maize. Ann Bot 97:695–704.
  • Brisson LF, Tenhaken R, Lamb C (1994) Function of oxidative cross-linking of cell walls structural proteins in plant disease resistance. Plant Cell 6:1703–1712.
  • Chen XP, Kong WD, He JZ, Liu WJ, Smith SE, Smith FA, Zhu YG (2008) Do water regimes affect iron-plaque formation and microbial communities in the rhizosphere of paddy rice? J Plant Nutri Soil Sci 171:193–199.
  • Clark LH, Harris WH (1981) Observations on the root anatomy of rice (Oryza sativa L.). Am J Bot 68:154–161.
  • Colmer TD (2003a) Long-distance transport of gases in plants: a perspective on internal aeration and radial oxygen loss from roots. Plant, Cell Environ 26:17–36.
  • Colmer TD (2003b) Aerenchyma and an inducible barrier to radial oxygen loss facilitate root aeration in upland, paddy and deepwater rice (Oryza sativa L.). Ann Bot 91(S):301–309.
  • Crowder A, Maacfis SM (1986) Seasonal deposition of ferric hydroxide plaque on roots of wetland plant. Can J Bot 64:2120–2124.
  • Dat J, Vandenabeele S, Vranová E, Van Montagu M, Inzé D, Van Breusegem F (2000) Dual action of the active oxygen species during plant stress responses. Cell Mol Life Sci 57:779–795.
  • Deng H, Ye ZH, Wong MH (2009) Lead, zinc and iron (Fe²⁺) tolerances in wetland plants and relation to root anatomy and spatial pattern of ROL. Environ Exp Bot 65:353–362.
  • Deng D, Wu SC, Wu FY, Deng H, Wong MH (2010) Effects of root anatomy and Fe plaque on arsenic uptake by rice seedlings grown in solution culture. Environ Pollut 158:2589–2595.
  • Dias MA, Costa MM (1983) Effect of low salt concentrations on nitrate reductase and peroxidase of sugar beet leaves. J Exp Bot 34:537–543.
  • Drew MC, He CJ, Morgan PW (1989) Decreased ethylene biosynthesis, and induction of aerenchyma, by nitrogen- or phosphatestarvation in adventitious roots of Zea mays L. Plant Physiol 91:266–271.
  • Enstone DE, Peterson CA (2005) Suberin lamella development in maize seedling roots grown in aerated and stagnant conditions. Plant, Cell Environ 28:444–455.
  • Fan M, Zhu J, Richards C, Brown KM, Lynch JP (2003) Physiological roles for aerenchyma in phosphorus-stressed roots. Func Plant Biol 30:493–506.
  • Fang WC, Wang JW, Lin CC, Kao CH (2001) Iron induction of lipid peroxidation and effects on antioxidative enzyme activities in rice leaves. Plant Growth Regul 35:75–80.
  • Foyer CH, Noctor G (2005) Oxidant and antioxidant signaling in plants: a re-evaluation of the concept of oxidative stress in a physiological context. Plant, Cell Environ 28:1056–1071.
  • Fu Y, Yu Z, Cai K, Shen H (2010) Mechanisms of iron plaque formation on root surface of rice plants and their ecological and environmental effects: a review (in Chinese). Plant Nutri Fert Sci 16:1527–1534.
  • Guo Z, Tan H, Zhu Z, Lu S, Zhou B (2005) Effect of intermediates on ascorbic acid and oxalate biosynthesis of rice and in relation to its stress resistance. Plant Physiol Biochem 43:955–962.
  • Insalud N, Bell RW, Colmer TD, Rerkasem B (2006) Morphological and physiological responses of rice (Oryza sativa) to limited phosphorus supply in aerated and stagnant solution culture. Ann Bot 98:995–1004.
  • Jabs T (1999) Reactive oxygen intermediates as mediators of programmed cell death in plants and animals. Biochem Pharmacol 57:231–245.
  • Jackson MB, Armstrong W (1999) Formation of aerenchyma and the processes of plant ventilation in relation to soil flooding and submergence. Plant Biol 1:274–287.
  • Kato M, Shimizu S (1987) Chlorophyll metabolism in higher plants. VII. Chlorophyll degradation in senescing tobacco leaves; phenolic-dependent peroxidative degradation. Can J Bot 65:729–735.
  • Kirk JGD (2004) Rice root properties for internal aeration and efficient nutrient acquisition in submerged soil. New Phytol 159:185–194.
  • Kirk JGD, Du LV (1997) Changes in rice root architecture, porosity, and oxygen and proton release under phosphorus deficiency. New Phytol 135:191–200.
  • Kludze HK, De Laune RD, Patrick WH Jr (1993) Aerenchyma formation and methane and oxygen exchange in rice. Soil Sci Soc Am J 57:386–391.
  • Kotula L, Ranathunge K, Schreiber L, Steudle E (2009) Functional and chemical comparison of apoplastic barriers to radial oxygen loss in roots of rice (Oryza sativa L.) grown in aerated or deoxygenated solution. J Exp Bot 60:2155–2167.
  • Li C, Qi X, Li M, Zhao G, Hu X (2009) Phosphate facilitates Fe(II) oxidative deposition in pea seed (Pisum sativum) ferritin. Biochimie 91:1475–1481.
  • Liu W, Hu Y, Zhu Y, Gao R, Zhao Q (2008) The mechanisms of iron plaque formation on the surface of rice roots induced by phosphorus starvation (in Chinese). Plant Nutri Fert Sci 14:22–27.
  • Longstreth DJ, Borkhsenious ON (2000) Root cell ultrastructure in developing aerenchyma tissue of three wetland species. Ann Bot 86:641–646.
  • Lynch JP, Brown K (1998) Regulation of root architecture by phosphorous availability. In: Lynch J, Deikman J (eds) Phosphorous in plant biology: regulatory roles ecosystem, organismic, cellular and molecular processes. ASPP, Rockville, pp 148–156.
  • Lynch JP, Brown KM (2008) Root strategies for phosphorus acquisition. In: White PJ, Hammond JP (eds) The ecophysiology of plant-phosphorus interactions. Springer, Dordrecht, pp 83–116.
  • Mendelssohn IA, Kleiss BA, Wakeley JS (1995) Factors controlling the formation of oxidized root channels: a review. Wetlands 15:37–46.
  • Mühlenbock P, Plaszczyca M, Plaszczyca M, Mellerowicz E, Karpinski S (2007) Lysigenous aerenchyma formation in arabidopsis is cont rolled by lesion simulating disease. Plant Cell 19:3819–3830.
  • O’Brien JA, Daudi A, Butt VS, Bolwell GP (2012) Reactive oxygen species and their role in plant defence and cell wall metabolism. Planta 236:765–779.
  • Passardi F, Cosio C, Penel C, Dunand C (2005) Peroxidases have more functions than a Swiss army knife. Plant Cell Rep 24:255–265.
  • Pi N, Tam NFY, Wu Y, Wong MH (2009) Root anatomy and spatial pattern of radial oxygen loss of eight true mangrove species. Aquat Bot 90:222–230.
  • Pochinok, Jing JH, Ding ZR (translated) (1981) Plant biochemistry analysis methods. (in Chinese) Science Press, Beijing.
  • Postma JA, Lynch JP (2011a) Theoretical evidence for the functional benefit of root cortical aerenchyma in soils with low phosphorus availability. Ann Bot 107:829–841.
  • Postma JA, Lynch JP (2011b) Root cortical aerenchyma enhances the growth of maize on soils with suboptimal availability of nitrogen, phosphorus, and potassium. Plant Physiol 156: 1190–1201.
  • Quan LJ, Zhang B, Shi WW, Li HY (2008) Hydrogen peroxide in plants: a versatile molecule of the reactive oxygen species network. J Integr Plant Biol 50:2–18.
  • Sagi M, Fluhr R (2001) Superoxide production by plant homologues of the gp91phox NADPH oxidase: modulation of activity by calcium and by tobacco mosaic virus infection. Plant Physiol 126:1281–1290.
  • Shiono K, Ogawa S, Yamazaki S, Isoda H, Fujimura T, Nakazono M, Colmer TD (2011) Contrasting dynamics of radial O₂-loss barrier induction and aerenchyma formation in rice roots of two lengths. Ann Bot 107:89–99.
  • Siqueira-Silva AI, da Silva LC, Azedevo AA, Oliva MA (2012) Iron plaque formation and morphoanatomy of roots from species of restinga subjected to excess iron. Ecotox Environ Safe 78:265–275.
  • Siyiannis VF, Protonotarios VE, Zechmann B, Chorianopoulou SN, Müller M, Hawkesford MJ, Bouranis DL (2012) Comparative spatiotemporal analysis of root aerenchyma formation processes in maize due to sulphate, nitrate or phosphate deprivation. Protoplasma 249:671–686.
  • Smolers AJP, Roelofs JGM (1996) The roles of internal iron hydroxide precipitation, sulphide toxicity and oxidizing ability in the survival of Stratiotes aloides roots at different iron concentrations in sediment pore water. New Phytol 133:253–260.
  • Srivastava S, Dubey RS (2011) Manganese-excess induces oxidative stress, lowers the pool of antioxidants and elevates activities of key antioxidative enzymes in rice seedlings. Plant Growth Regul 64:1–16.
  • Thordal-Christensen H, Zhang Z, Wei Y, Collinge DB (1997) Subcellular localization of H₂O₂ in plants. H₂O₂ accumulation in papillae and hypersensitive response during the barley-powdery mildew interaction. Plant J 11:1187–1194.
  • Thounaojam TC, Pandaa P, Mazumdar P, Kumar D, Sharma GD, Sahoo L, Panda SK (2012) Excess copper induced oxidative stress and response of antioxidants in rice. Plant Physiol Biochem 53:33–39.
  • van Breusegem F, Vranová E, Dat JF, Inzé D (2001) The role of active oxygen species in plant signal transduction. Plant Sci 161:405–414.
  • Velikova V, Yordanov I, Edreva A (2000) Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Protective role of exogenous polyamines. Plant Sci 151:59–66.
  • Wang AG, Luo GH (1990) Quantitative relation between the reaction of hydroxylaminne and superoxide anion radicals in plants (in Chinese). Plant Physiol Commun 26(6):55–57.
  • Wenlinder KG (1992) Superfamily of plant, fungal and bacterial peroxidases. Curr Opin Struc Biol 2:388–393.
  • Willekens H, Chamnongpol S, Davey M, Schraudner M, Langebartels C, Montagu MV, Inzé D, Camp WV (1997) Catalase is a sink for H₂O₂ and is indispensable for stress defence in C₃ plants. EMBO J 16:4806–4816.
  • Wu C, Ye Z, Shu W, Zhu Y, Wong M (2011) Arsenic accumulation and speciation in rice are affected by root aeration and variation of genotypes. J Exp Bot 62:2889–2898.
  • Xu SC, Ding HD, Sang JR (2007) Reactive oxygen species, metabolism, and signal transduction in plant cells. Acta Bot Yunn 29:355–365.
  • Yamada H, Takeda C, Mizushima A, Yoshino K, Yonebayashi K (2005) Effect of oxidizing power of roots on iodine uptake by rice plants. Soil Sci Plant Nutrit 61:141–145.
  • Yoshida S, Douglas A, Forno JC (1976) Laboratory manual for physiological studies of rice. IRRI, Los Banos.
  • Yu F, Xu D, Lei R, Li N, Li KA (2008) Free-radical scavenging capacity using the Fenton reaction with Rhodamine B as the spectrophotometric indicator. J Agri Food Chem 56:730–735.
  • Zhang Y, Zheng GH, Liu P, Song JM, Xu GD, Cai MZ (2011) Morphological and physiological responses of root tip cells to Fe²⁺ toxicity in rice. Acta Physiol Plant 33:683–689.
  • Zhao F, Wang DY, Xu CM, Zhang WJ, Li FB, Mao HJ, Zhang XF (2010) Response of morphological, physiological and yield characteristics of rice (Oryza sativa L.) to different oxygenincreasing patterns in rhizosphere (in Chinese with English abstract). Acta Agron Sin 36:303–312.

Typ dokumentu



Identyfikator YADDA

JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.