3D-analysis of plant microstructures: advantages and limitations of synchrotron X-ray microtomography

• Autorzy: Matsushima U. , Graf W. , Zabler S. , Manke I. , Dawson M. , Choinka G. , Hilger A. , Herppich W. B.
• Języki publikacji: EN

Abstrakty

EN

Synchrotron X-ray computer microtomography was used to analyze the microstructure of rose peduncles. Samples from three rose cultivars, differing in anatomy, were scanned to study the relation between tissue structure and peduncles mechanical strength. Additionally, chlorophyll fluorescence imaging and conventional light microscopy was applied to quantify possible irradiation-induced damage to plant physiology and tissue structure. The spatial resolution of synchrotron X-ray computer microtomography was sufficiently high to investigate the complex tissues of intact rose peduncles without the necessity of any preparation. However, synchrotron X-radiation induces two different types of damage on irradiated tissues. First, within a few hours after first X-ray exposure, there is a direct physical destruction of cell walls. In addition, a slow and delayed destruction of chlorophyll and, consequently, of photosynthetic activity occurred within hours/ days after the exposure. The results indicate that synchrotron X-ray computer microtomography is well suited for three-dimensional visualization of the microstructure of rose peduncles. However, in its current technique, synchrotron X-ray computer microtomography is not really non-destructive but induce tissue damage. Hence, this technique needs further optimization before it can be applied for time-series investigations of living plant materials.

• Wydawca: -
• Czasopismo: International Agrophysics
• Rocznik: 2013
• Tom: 27
• Numer: 1
• Opis fizyczny: p.23-30,fig.,ref.

Twórcy

autor

Matsushima U.

• Faculty of Agriculture, Iwate University, 3-18-8, Ueda, Morioka, Iwate, 020-8550, Japan

autor

Graf W.

• Department of Horticultural Engineering, Leibniz Institute for Agricultural Engineering Potsdam-Bornim, Max-Eyth-Allee 100, 14469 Potsdam, Germany
• Kuratorium fur Technik und Bauwesen in der Landwirtschaft, Bartningstrasse 49, 64289 Darmstadt, Germany

autor

Zabler S.

• Institute for Physics and Astronomy – Chair for X-ray microscopy, Julius-Maximillians Universitaty of Wurzburg, Campus Hubland Nord, Josef-Martin-Weg 63, 97074 Wurzburg, Germany

autor

Manke I.

• SF3, Helmholtz Center Berlin for Materials and Energy, Hahn-Meitner-Platz 1, 14109 Berlin, Germany

autor

Dawson M.

• University of Salford, Newton Building 164, Salford, Greater Manchester, M5 4WT, United Kingdom

autor

Choinka G.

• SF3, Helmholtz Center Berlin for Materials and Energy, Hahn-Meitner-Platz 1, 14109 Berlin, Germany

autor

Hilger A.

• SF3, Helmholtz Center Berlin for Materials and Energy, Hahn-Meitner-Platz 1, 14109 Berlin, Germany

autor

Herppich W. B.

• Department of Horticultural Engineering, Leibniz Institute for Agricultural Engineering Potsdam-Bornim, Max-Eyth-Allee 100, 14469 Potsdam, Germany

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