Agrobacterium - mediated genetic transformation of Withania somnifera using nodal explants

• Autorzy: Udayakumar R. , Kasthurirengan S. , Mariashibu T.S. , Sahaya Rayan J.J. , Ganapathi A. , Kim S.C. , Kim J.J. , Choi C.W.
• Języki publikacji: EN

Abstrakty

EN

Withania somnifera is an important medicinal plant and used to cure many diseases. Direct regeneration method was standardized for the nodal explants of W. somnifera. In this method, the maximum of 42.4 ± 2.68 shoots produced per explant was achieved at 1.5 mg l⁻¹ BAP with 0.3 mg l⁻¹ IAA in the second subculture. Transformation was performed in the nodal explants of W. somnifera via direct regeneration using Agrobacterium tumefaciens strain EHA105 that harbored a binary vector pGA492, which carrying kanamycin resistant (nptII), phosphinothricin resistant (bar) and an intron containing β-glucuronidase (gus-intron) genes. The sensitivity of nodal explants to kanamycin (300 mg l⁻¹) was determined for the selection of transformed plants. Transformation was confirmed by histochemical β-glucuronidase (GUS) assay and amplification of the nptII gene by polymerase chain reaction (PCR). PCR and southern blot analyses confirmed the integration of nptII gene in the genome of W. somnifera and the transformation frequency of 3.16 % has been achieved. This is the first report on the genetic transformation of W. somnifera using nodal explants, which may aid in the transformation of any other character gene for improving therapeutic value.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 08
• Opis fizyczny: p.1969-1980,fig.,ref.

Twórcy

autor

Udayakumar R.

• Department of Biochemistry, Government Arts College (Autonomous), 612 001 Kumbakonam, Tamil Nadu, India
• Department of Biochemistry, Bharathidasan University, 620 024 Tiruchirappalli, Tamilnadu, India
• Department of Bioogy and Medicinal Science, Pai Chai University, 302-735 Daejeon, Korea

autor

Kasthurirengan S.

• Department of Biochemistry, Bharathidasan University, 620 024 Tiruchirappalli, Tamilnadu, India

autor

Mariashibu T.S.

• Department of Biochemistry, Bharathidasan University, 620 024 Tiruchirappalli, Tamilnadu, India

autor

Sahaya Rayan J.J.

• Department of Biochemistry, Bharathidasan University, 620 024 Tiruchirappalli, Tamilnadu, India

autor

Ganapathi A.

• Department of Biochemistry, Bharathidasan University, 620 024 Tiruchirappalli, Tamilnadu, India

autor

Kim S.C.

• Department of Bioogy and Medicinal Science, Pai Chai University, 302-735 Daejeon, Korea

autor

Kim J.J.

• Department of Bioogy and Medicinal Science, Pai Chai University, 302-735 Daejeon, Korea

autor

Choi C.W.

• Department of Bioogy and Medicinal Science, Pai Chai University, 302-735 Daejeon, Korea

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Identyfikatory

DOI

10.1007/s11738-014-1572-2