On the mode of integration of the thylakoid membrane protein cytochrome b6 into cytoplasmic membrane of Escherichia coli

• Autorzy: Kroliczewski J. , Gubernator B. , Rogner M. , Szczepaniak A.
• Języki publikacji: EN

Abstrakty

EN

 In the stroma compartment, several pathways are used for integration/translocation of chloroplast proteins into or across the thylakoid membrane. In this study we investigated the mode of incorporation of the chloroplast-encoded cytochrome b6 into the bacterial membrane. Cytochrome b6 naturally comprises of four transmembrane helices (A,B,C,D) and contains two b-type hemes. In the present study, mature cytochrome b6 or constructed deletion mutants of cytochrome were expressed in E. coli cells. The membrane insertion of cytochrome b6 in this bacterial model system requires an artificially added presequence that directs the protein to use an E. coli membrane-insertion pathway. This could be accomplished by fusion to maltose-binding protein (MBP) or to the bacterial Sec-dependent signal peptide (SSpelB). The integration of mature cytochrome b6 into the bacterial cytoplasmic membrane by the Sec pathway has been reported previously by our group (Kroliczewski et al., 2005, Biochemistry, 44: 7570). The results presented here show that cytochrome b6 devoid of the first helix A can be inserted into the membrane, as can the entire ABCD. On the other hand, the construct devoid of helices A and B is translocated through the membrane into the periplasm without any effective insertion. This suggests the importance of the membrane-anchoring sequences that are likely to be present in only the A and B part, and it is consistent with the results of computational prediction which did not identify any membrane-anchoring sequences for the C or D helices. We also show that the incorporation of hemes into the truncated form of cytochrome b6 is possible, as long as the B and D helices bearing axial ligands to heme are present.

Słowa kluczowe

EN

thylakoid membrane; membrane protein; cytochrome b6; cytoplasmic membrane; Escherichia coli; integration; translocation; signal sequence; chloroplast protein

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2011
• Tom: 58
• Numer: 3

Twórcy

autor

Kroliczewski J.

• Laboratory of Biophysics, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland

autor

Gubernator B.

autor

Rogner M.

autor

Szczepaniak A.

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