Novel method of detection of phenylpropanoids of Rhodiola roots species

• Autorzy: Gryszczynska A. , Opala B. , Krajewska-Patan A. , Lowicki Z. , Buchwald W. , Mielcarek S. , Bogacz A. , Karasiewicz M. , Boron D. , Czerny B. , Mrozikiewicz P. M.

Warianty tytułu

PL

Nowa metoda oznanczania zawartości fenylopropanoidów w korzeniach z gatunku Rhodiola

• Języki publikacji: EN

Abstrakty

EN

The aim of the study was the identification and quantitative analysis of phenylpropanoid compounds in the roots of Rhodiola species. Rosavin, rosarin and rosin were determined in the roots of R. kirilowii and R. rosea from the field cultivation, Institute of Natural Fibres and Medicinal Plants. For the quantitative analysis, the ultra performance liquid chromatography - tandem mass spectrometry (UPLC-ESI MS/MS, Waters) was used. The results showed differences in the quantitative and qualitative assessments of these two species. In the root of R. kirilowii the presence of phenylpropanoids was not confirmed. In R. rosea the most common phenylpropanoid was rosavin (0.022%). The UPLC-MS/MS studies allowed to use this analytical method for determination of phenylpropanoids in the accordance with the requirements of ICH.

PL

Celem przeprowadzonych badań w ramach projektu badawczego było opracowanie metody analitycznej pozwalającej na oznaczenie trzech związków fenylopropanoidów w dwóch gatunkach różeńca. Do detekcji rozawiny, rozyny i rozaryny wykorzystano wysokosprawny chromatograf cieczowy sprzężony z tandemowym spektrometrem mas (UPLC-MS/MS). Obydwa gatunki różeńca Rhodiola kirilowii oraz Rhodiola rosea zostały zebrane z upraw prowadzonych w Instytucie Włókien Naturalnych i Roślin Zielarskich w Poznaniu w 2009 r. Dodatkowo z tych surowców przygotowano po dwa wyciągi: wyciąg wodny oraz wodnoalkoholowy (50% etanol), które następnie przebadano pod względem zawartości fenylopropanoidów. Wszystkie przeprowadzone analizy potwierdziły możliwość wykorzystania tej metody do oznaczenia zawartości fenylopropanoidów w rodzaju Rhodiola.

Słowa kluczowe

EN

new method; detection method; phenylpropanoid; quantitative analysis; identification; Rhodiola kirilowii; Rhodiola rosea; Crassulaceae; root; ultra performance liquid chromatography-tandem mass spectrometric method

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2013
• Tom: 59
• Numer: 1
• Opis fizyczny: p.17-28,fig.,ref.

Twórcy

autor

Gryszczynska A.

• Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland

autor

Opala B.

• Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland

autor

Krajewska-Patan A.

• Department of Pharmacology and Experimental Biology, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland

autor

Lowicki Z.

• Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland

autor

Buchwald W.

• Team of Botany and Agriculture of Medicinal Plants, Department of Botany, Breeding and Agricultural Technology, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2, 62-064 Plewiska/Poznan, Poland

autor

Mielcarek S.

• Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland

autor

Bogacz A.

• Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland
• Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, Poznan University of Medical Sciences, Sw. Marii Magdaleny 14, 61-861 Poznan, Poland

autor

Karasiewicz M.

• Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland

autor

Boron D.

• Depertment of Histology and Embriology, Medical University of Silesia, Jordana 19, 41-808 Zabrze, Poland

autor

Czerny B.

• Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland
• Department of General Pharmacology and Pharmacoeconomics, Pomeranian Medical University, Zolnierska 48, 70-204 Szczecin, Poland

autor

Mrozikiewicz P. M.

• Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland
• Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, Poznan University of Medical Sciences, Sw. Marii Magdaleny 14, 61-861 Poznan, Poland

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