Ocena skuteczności drożdży Aureobasidium pullulans, Debaryomyces hansenii i Rhodotorula glutinis w ograniczeniu septoriozy paskowanej liści pszenicy (Zymoseptoria tritici)

• Autorzy: Wachowska U. , Duba A. , Goriewa K. , Wiwart M.

Warianty tytułu

EN

The effectiveness of Aureobasidium pullulans, Debaryomyces hansenii and Rhodotorula glutinis yeasts in inhibiting the development of Septoria leaf blotch (Zymoseptoria tritici) in wheat

• Języki publikacji: PL

Abstrakty

PL

Grzyb Zymoseptoria tritici, sprawca septoriozy paskowanej liści, jest jednym z najgroźniejszych patogenów pszenicy między innymi z powodu bardzo długiego okresu inkubacji w tkankach roślin (faza bezobjawowa trwa nawet 10 dni) oraz bardzo szybkiego tempa rozprzestrzeniania się na plantacji, a także trudności w jego zwalczaniu metodami chemicznymi. Jedną z naturalnych metod ograniczenia rozwoju tego patogenu jest uprawa odmian o mniejszej podatności na infekcję. Alternatywą jest także poszukiwanie skutecznych biologicznych metod ochrony. Doświadczenia polowe prowadzono w dwóch lokalizacjach w północno-wschodniej Polsce na dwóch odmianach Triticum aestivum (‘Tonacja’ i ‘Skagen’) i jednej T. durum (‘Komnata’). Celem badań była ocena skuteczności zabiegów biologicznych polegających na opryskiwaniu roślin zawiesiną drożdży Aureobasidium pullulans, Debaryomyces hansenii i Rhodotorula glutinis w ograniczeniu objawów septoriozy paskowanej liści pszenicy. Odmiana Komnata charakteryzowała się mniejszą podatnością na infekcje liści Z. tritici niż odmiany Skagen i Tonacja. Skuteczność zabiegów biologicznych w ograniczeniu objawów septoriozy paskowanej liści odmiany Tonacja oszacowano na poziomie 59,3% dla izolatu A. pullulans oraz 56,7% dla D. hansenii. Zabiegi biologiczne nie miały istotnego wpływu na plonowanie pszenicy.

EN

The causal agent of Septoria leaf blotch is one of the most dangerous fungal pathogens of wheat. It has a very long incubation period in plant tissues, spreads rapidly in crops and is highly resistant to chemical agents. Biological control methods could be used together with fungicides, but only between ten and twenty biocontrol agents have been approved for use in Europe to date. The aim of this study was to evaluate the effectiveness of foliar-applied cell suspensions of Aureobasidium pullulans, Debaryomyces hansenii and Rhodotorula glutinis yeasts in eliminating the symptoms of Septoria leaf blotch in winter wheat. A field experiment was carried out in two locations in north-eastern Poland on two cultivars of Triticum aestivum (‘Tonacja’ and ‘Skagen’) and one T. durum cultivar (‘Komnata’). The yeast isolates used in the experiment were obtained from the grain of winter wheat cv. Tonacja (A. pullulans, Rh. glutinis) and apples cv. Antonówka (D. hansenii). The ITS1-5,8SrRNA-ITS2 sequences of A. pullulans, D. hansenii and R. glutinis yeasts were deposited in the NCBI database under accession numbers KX444670, KX444669 and KX444653, respectively. Yeast suspensions with density of 0.4–2.5·105 cells per cm3 of sterile water were sprayed onto plants with the use of a backpack sprayer at the first node stage, at the heading stage and at the full flowering stage. The effectiveness of biological treatments in controlling the spread of Septoria leaf depended on the susceptibility of wheat cultivars to infections caused by Zymoseptoria tritici and the yeast strain used as a biocontrol agent. Durum wheat cv. Komnata was less susceptible to leaf infections caused by Z. tritici than common wheat cvs. Skagen and Tonacja. In all analyzed wheat cultivars, the severity of Septoria leaf blotch was considerably lower on flag leaves than on penultimate leaves. The effectiveness of biological treatments in controlling the spread of Septoria leaf blotch in common wheat cv. Tonacja was estimated at 59.3% for the suspension containing the Aureobasidium pullulans isolate and 56.7% for the suspension containing the Debaryomyces hansenii isolate. In the remaining wheat cultivars, the effectiveness of biological treatments did not exceed 54.5%, and it was not statistically significant, mostly due to the low density of the cell suspension of the Rhodotorula glutinis isolate, which was characterized by a low growth rate on agar. Biological treatments had no significant influence on wheat yield. Since yeasts used as biocontrol agents are not highly effective against Z. tritici, they should be combined with chemical methods.

• Wydawca: -
• Czasopismo: Zeszyty Problemowe Postępów Nauk Rolniczych
• Rocznik: 2018
• Tom: 592
• Opis fizyczny: s.97-106,tab.,bibliogr.

Twórcy

autor

Wachowska U.

• Wydział Kształtowania Środowiska i Rolnictwa, Uniwersytet Warmińsko-Mazurski w Olsztynie

autor

Duba A.

• Wydział Kształtowania Środowiska i Rolnictwa, Uniwersytet Warmińsko-Mazurski w Olsztynie

autor

Goriewa K.

• Wydział Kształtowania Środowiska i Rolnictwa, Uniwersytet Warmińsko-Mazurski w Olsztynie

autor

Wiwart M.

• Wydział Kształtowania Środowiska i Rolnictwa, Uniwersytet Warmińsko-Mazurski w Olsztynie

Bibliografia

• Bargabus R.L., Zidack N.K., Sherwood J.E., Jacobsen B.J., 2003. Oxidative burst elicited by Bacillus mycoides isolate Bac J, a biological control agent, occurs independently of hypersensitive cell death in sugar beet. Mol. Plant Microbe Interact. 16, 1145–1153.
• Castoria R., De Curtis F., Lima G., Caputo L., Pacifico S., De Cicco V., 2001. Aureobasidium pullulans (LS-30) an antagonist of postharvest pathogens of fruits: Study on its modes of action. Postharvest Biol. Technol. 22(1), 1.
• Curvers K., Pycke B., Kyndt T., Vanrompay D., Haesaert G., Gheysen G., 2015. A high resolution melt (HRM) assay to characterize CYP51 haplotypes of the wheat pathogen Mycosphaerella graminicola. Crop Prot. 71, 12–18.
• Droby S., Chalutz E., Wilson C. L., Wisniewski M., 1989. Characterization of the biocontrol activity of Debaryomyces hansenii in the control of Penicillium digitatum on grapefruit. Can. J. Microbiol. 35(8), 794–800.
• Eriksen L., Munk L., 2003. The occurrence of Mycosphaerella graminicola and its anamorph Septoria tritici in winter wheat during the growing season. Eur. J. Plant Pathol. 109, 253–259.
• Fraaije B.A., Lucas J.A., Clark W.S., Burnett F.J., 2003. QoI resistance development in populations of cereal pathogens in the UK. The BCPC International Congress-Crop Science & Technology, 689–694.
• Gerbore J., Benhamou N., Vallance J., Le Floch G., Grizard D., Regnault-Roger C., Rey P. 2014. Biological control of plant pathogens: advantages and limitations seen through the case study of Pythium oligandrum. Environ. Sci. Pollut. Res. 21, 4847–4860.
• https://bip.minrol.gov.pl/.../fung%20-%20choroby%20liści%20 zbóż [dostęp: 02.06.2016].
• Ippolito A., El Ghaouth A., Wilson CL., Wisniewski M., 2000. Control of postharvest decay of apple fruit by Aureobasidium pullulans and induction of defense responses. Postharvest Biol. Technol. 19, 265–272.
• Kildea S., Ransbotyn V., Khan M., Fagan B., Leonard G., Mullins E., Doohan F.M., 2008. Bacillus megaterium shows potential for the biocontrol of Septoria tritici blotch of wheat. Biol. Control. 47, 37–45.
• Kurtzman C., Fell J.W., Boekhout T., 2011. The yeasts: a taxonomic study. Elsevier, London.
• Leroux P., Albertini C., Gautier A., Gredt M., Walker A.S., 2007. Mutations in the cyp51 gene correlated with changes in sensitivity to sterol 14α-demethylation inhibitors in field isolates of Mycosphaerella graminicola. Pest. Manag. Sci. 63, 688–699.
• Lima G., De Curtis F., Castoria R., De Cicco V., 2003. Integrated control of apple postharvest pathogens and survival of biocontrol yeasts in semi-commercial conditions. Eur. J. Plant Pathol. 109, 341–349.
• Lovell D.J., Hunter T., Powers S.J., Parker S. R., Van den Bosch F., 2004. Effect of temperature on latent period of septoria leaf blotch on winter wheat under outdoor conditions. Plant Pathol. 53(2), 170–181.
• Mavroeidi V.I., Shaw M.W., 2005. Sensitivity distributions and cross-resistance patterns of Mycosphaerella graminicola to fluquiconazole, prochloraz and azoxystrobin over a period of 9 years. Crop Prot. 24, 259–299.
• Mirzwa-Mróz E., Tvaružek L., Zamorski C., Nowicki B., 2005. Research on the development of Mycosphaerella graminicola (Fuckel) Schroeter teleomorph on wheat leaves. Acta Agrobot. 58(1), 59–65.
• Perelló A.E., Moreno M.V., Mónaco C., Simón M.R., Cordo C., 2009. Biological control of Septoria tritici blotch on wheat by Trichoderma spp. under field conditions in Argentina. BioControl. 54, 113–122.
• Rodrigo S., Cuello-Hormigo B., Gomes C., Santamaria O., Costa R., Poblaciones M.J., 2015. Influence of fungicide treatments on disease severity caused by Zymoseptoria tritici, and on grain yield and quality parameters of bread-making wheat under Mediterranean conditions. Eur. J. Plant Pathol. 141(1), 99–109.
• Rudd J.J., 2015. Previous bottlenecks and future solutions to dissecting the Zymoseptoria tritici – wheat host-pathogen interaction. Fungal Genet. Biol. 79, 24–28.
• Ryu C.M., Fara M.A., Hu C.H., Reddy M.S., Kloepper J.W., Pare P.W., 2004. Bacterial volatiles induce systemic resistance in Arabidopsis. Plant Physiol. 134, 1017–1026.
• Slightom J.L., Metzger B.P., Luu H.T., Elhammer A.P., 2009. Cloning and molecular characterization of the gene encoding the aureobasidin A bosynthesis complex in Aureobasidium pullulans BP-1938. Gene 431 67–79.
• Torriani S.F.F., Brunner P.C., McDonal B.A., Sierotzki H., 2008. Qol resistance emerged independently at least 4 times in European populations of Mycosphaerella graminicola. Pest Manag. Sci. 65, 155–162.
• Wachowska U., Borowska J., 2014. Antagonistic yeasts competes for iron with winter wheat stem base pathogens. Gesunde Pflanz. 66, 141–148.
• Wang W., Chi Z., Liu G., Buzdar M.A., Chi Z., 2009. Chemical and biological characterization of siderophore produced by the marine-derived Aureobasidium pullulans HN6.2 and its antibacterial activity. BioMetals 22, 965–972.
• White T.J., Bruns T., Lee S., Taylor J.W., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, 315-322. In: PCR Protocols: A Guide to Methods and Applications, M.A. Innis, D.H. Gelfand, J.J. Sninsky, T.J. White (eds.). Academic Press, New York.
• Witzenberger A., Hack H., van den Boom T., 1989. Erläuterungen zum BBCH-Dezimal-Code für die Entwicklungsstadien des Getreides – mit Abbildungen. Gesunde Pflanz. 41, 384–388.
• Zalar P., Gostinčar C., de Hoog G.S., Uršič V., Sudhadham M., Gunde-Cimerman N., 2008. Redefinition of Aureobasidium pullulans and its varieties. Stud. Mycol. 61, 21–38.
• Żarowska B., 2012. Biosynteza i charakterystyka toksyn killerowych drożdży Debaryomyces hansenii. Wydaw. UP, Wrocław.

Identyfikatory

DOI

10.22630/ZPPNR.2018.592.9