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2015 | 18 | 3 |

Tytuł artykułu

Dynamic expression profile of DNA methyltransferases in rat testis development

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
DNA methyltransferases (Dnmts) are unique and perform specific functions during male germ cell development. To further characterize the significance of Dnmts in the events leading to production of spermatozoa, we investigated whether the expression patterns in Dnmt1, Dnmt3a, Dnmt3b and Dnmt3l were apparent in rat testes at different time points during development. The qRT-PCR results showed that expression levels of Dnmt3a and Dnmt3l were abundant before birth and were present at the highest levels in testes tissue at 18.5 days postcoitus (dpc), and gradually decreased from day 0 postpartum (dpp) to 90 dpp. Expression of Dnmt1 and Dnmt3b reached a peak after birth (P <0.01), and then gradually reduced until adulthood. Western blotting and immunolocalization analysis of Dnmt3a and Dnmt3b further confirmed the differential expression and localization of the two proteins during rat testis development. The dynamic expression profile of Dnmts implies specific and potentially nonredundant roles for each of these enzymes in the developing rat testis.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

18

Numer

3

Opis fizyczny

p.549-556,fig.,ref.

Twórcy

autor
  • College of Animal Science and Technology, Northwest A and F University, 22 Xinong Road, Yangling, Shaanxi 712100, P. R. China
  • Institute of Genetics, College of Life Sciences, Xinyang Normal University, 237 Nanhu Road, Xinyang, Henan, 464000, P. R. China
autor
  • College of Animal Science and Technology, Northwest A and F University, 22 Xinong Road, Yangling, Shaanxi 712100, P. R. China
autor
  • College of Animal Science and Technology, Northwest A and F University, 22 Xinong Road, Yangling, Shaanxi 712100, P. R. China
autor
  • College of Animal Science and Technology, Northwest A and F University, 22 Xinong Road, Yangling, Shaanxi 712100, P. R. China

Bibliografia

  • Bourc’his D, Bestor TH (2004) Meiotic catastrophe and retrotransposon reactivation in male germ cells lacking Dnmt3L. Nature 431: 96-99.
  • Bourc’his D, Xu GL, Lin CS, Bollman B, Bestor TH (2001) Dnmt3L and the establishment of maternal genomic imprints. Science 294: 2536-2539.
  • Cedar H, Bergman Y (2012) Programming of DNA methylation patterns. Annu Rev Biochem 81: 97-117.
  • Chedin F, Lieber MR, Hsieh CL (2002) The DNA methyltransferase-like protein DNMT3L stimulates de novo methylation by Dnmt3a. Proc Natl Acad Sci USA 99: 16916-16921.
  • Chen T, Ueda Y, Dodge JE, Wang Z, Li E (2003) Establishment and maintenance of genomic methylation patterns in mouse embryonic stem cells by Dnmt3a and Dnmt3b. Mol Cell Biol 23: 5594-5605.
  • Costa DS, Faria FJ, Fernandes CA, Silva JC, Auharek SA (2013) Testis morphometry and kinetics of spermatogenesis in the feral pig (Sus scrofa). Anim Reprod Sci 142: 63-70.
  • Culty M (2013) Gonocytes, from the fifties to the present: is there a reason to change the name? Biol Reprod 89: 46.
  • Goossens E, Bilgec T, Van Saen D, Tournaye H (2011) Mouse germ cells go through typical epigenetic modifications after intratesticular tissue grafting. Hum Reprod 26: 3388-3400.
  • Hata K, Okano M, Lei H, Li E (2002) Dnmt3L cooperates with the Dnmt3 family of de novo DNA methyltransferases to establish maternal imprints in mice. Development 129: 1983-1993.
  • Jia D, Jurkowska RZ, Zhang X, Jeltsch A, Cheng X (2007) Structure of Dnmt3a bound to Dnmt3L suggests a model for de novo DNA methylation. Nature 449: 248-251.
  • Jue K, Benoit G, Alcivar-Warren AA, Trasler JM (1995) Developmental and hormonal regulation of DNA methyltransferase in the rat testis. Biol Reprod 52: 1364-1371.
  • Jurkowska RZ, Jurkowski TP, Jeltsch A (2011) Structure and function of mammalian DNA methyltransferases. Chembiochem 12: 206-222.
  • Jurkowska RZ, Rajavelu A, Anspach N, Urbanke C, Jankevicius G, Ragozin S, Nellen W, Jeltsch A (2011) Oligomerization and binding of the Dnmt3a DNA methyltransferase to parallel DNA molecules: heterochromatic localization and role of Dnmt3L. J Biol Chem 286: 24200-24207.
  • Kaneda M, Okano M, Hata K, Sado T, Tsujimoto N, Li E, Sasaki H (2004) Essential role for de novo DNA methyltransferase Dnmt3a in paternal and maternal imprinting. Nature 429: 900-903.
  • Kotini AG, Mpakali A, Agalioti T (2011) Dnmt3a1 upregulates transcription of distinct genes and targets chromosomal gene clusters for epigenetic silencing in mouse embryonic stem cells. Mol Cell Biol 31: 1577-1592.
  • La Salle S, Mertineit C, Taketo T, Moens PB, Bestor TH, Trasler JM (2004) Windows for sex- specific methylation marked by DNA methyltransferase expression profiles in mouse germ cells. Dev Biol 268: 403-415.
  • La Salle S, Trasler JM (2006) Dynamic expression of DNMT3a and DNMT3b isoforms during male germ cell development in the mouse. Dev Biol 296: 71-82.
  • Lagos-Cabre R, Moreno RD (2008) Mitotic, but not meiotic, oriented cell divisions in rat spermatogenesis. Reproduction 135: 471-478.
  • Leblond CP, Clermont Y (1952) Definition of the stages of the cycle of the seminiferous epithelium in the rat. Ann N Y Acad Sci 55: 548-573.
  • Lees-Murdock DJ, De Felici M, Walsh CP (2003) Methylation dynamics of repetitive DNA elements in the mouse germ cell lineage. Genomics 82: 230-237.
  • Li E (2002) Chromatin modification and epigenetic reprogramming in mammalian development. Nat Rev Genet 3: 662-673.
  • Malkov M, Fisher Y, Don J (1998) Developmental schedule of the postnatal rat testis determined by flow cytometry. Biol Reprod 59: 84-92.
  • Neri F, Krepelova A, Incarnato D, Maldotti M, Parlato C, Galvagni F, Matarese F, Stunnenberg HG, Oliviero S (2013) Dnmt3L antagonizes DNA methylation at bivalent promoters and favors DNA methylation at gene bodies in ESCs. Cell 155: 121-134.
  • Oakes CC, La Salle S, Smiraglia DJ, Robaire B, Trasler JM (2007) A unique configuration of genome-wide DNA methylation patterns in the testis. Proc Natl Acad Sci U S A 104: 228-233.
  • Okano M, Bell DW, Haber DA, Li E (1999) DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell 99: 247-257.
  • Okano M, Xie S, Li E (1998) Cloning and characterization of a family of novel mammalian DNA (cytosine-5) methyltransferases. Nat Genet 19: 219-220.
  • Pontier DB, Gribnau J (2011) Xist regulation and function explored. Hum Genet 130: 223-236.
  • Reik W, Dean W (2001) DNA methylation and mammalian epigenetics. Electrophoresis 22: 2838-2843.
  • Sakai Y, Suetake I, Itoh K, Mizugaki M, Tajima S, Yamashina S (2001) Expression of DNA methyltransferase (Dnmt1) in testicular germ cells during development of mouse embryo. Cell Struct Funct 26: 685-691.
  • Sakai Y, Suetake I, Shinozaki F, Yamashina S, Tajima S (2004) Co-expression of de novo DNA methyltransferases Dnmt3a2 and Dnmt3L in gonocytes of mouse embryos. Gene Expr Patterns 5: 231-237.
  • Schaefer CB, Ooi SK, Bestor TH, Bourc’his D (2007) Epigenetic decisions in mammalian germ cells. Science 316: 398-399.
  • Shovlin TC, Bourc’his D, La Salle S, O’Doherty A, Trasler JM, Bestor TH, Walsh CP (2007) Sex-specific promoters regulate Dnmt3L expression in mouse germ cells. Hum Reprod 22: 457-467.
  • Smith ZD, Meissner A (2013) DNA methylation: roles in mammalian development. Nat Rev Genet 14: 204-220.
  • Urriola-Munoz P, Lizama C, Lagos-Cabre R, Reyes JG, Moreno RD (2014) Differential expression and localization of ADAM10 and ADAM17 during rat spermatogenesis suggest a role in germ cell differentiation. Biol Res 47: 31.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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